Effect of 2, 4-dichlorophenoxyacetic acid on callus induction in wheat (Triticum aestivum L.) genotypes

Abstract

Wheat is a member of family poaceae. Mature healthy seeds of four genotypes of wheat viz., PBW343, PBW373, RAJ 3765 and HD 2687 were inoculated on the MS media supplemented with 2, 4-D (2, 4-Dichlorophenoxy acetic acid). Four different combinations of 2, 4-D ranging from 5-8 mg L-1 was used for callus induction in four Indian varieties. Higher levels of 2,4-D were found to inhibit callus proliferation. Maximum callus induction was obtained in PBW343 (90.0%) at 6.0 mg L-1 2, 4-D followed by HD2687 (65.0%). Good embryoid production was apparent in third and fourth weeks of callus subculture. Average fresh weights of the calli for PBW 343 and HD2967 were 0.31 and 0.27 g per explant source, respectively.