Salt stress induced changes in protein profile of tolerant (Kh 65) and sensitive (HD 2009) wheat genotypes

Abstract

In addition to drought and extreme temperatures, soil salinity
represents a growing threat to wheat crop productivity. Therefore,
understanding molecular mechanism involved in salinity stress can
help in developing salt tolerant cultivars. In this investigation a
comparative proteome analysis using 2-D electrophoresis was carried
out between salt sensitive variety HD 2009 and salt tolerant variety
Kh 65 to identify differentially expressed protein subunits. Salt
treatment (12 ECe) was given to 2 week old seedlings under controlled
conditions in Growth Chamber. Chemical and physical parameters
were recorded after 72 hrs of salt treatment. 2D- electrophoresis of
proteins extracts from root tissues was conducted under control and
treated conditions. There was reduction in root biomass by 32 and 56
per cent in Kh 65 and HD 2009, respectively. A total of more than
200 spots were identified on the gel among them 60 spots showed
differential expression in both the cultivars in combination. Among
them, seventeen spots (3, 6, 8, 9, 11, 12, 14, 15, 21, 23, 25, 27, 30, 33,
30, 35 and 36) were up-regulated and while fifteen (2, 4, 5, 7, 13, 16,
17, 18, 20, 22, 26, 31, 37, 38 and 39) down-regulated in Kh65. Whereas
only five spots (4, 35, 36, 58, 59) were up-regulated in HD 2009 and
twenty one (1, 2, 3, 5, 9, 26, 37, 38, 40, 42, 43, 44, 45, 46, 47, 49, 52,
53, 54, 56, and 60)down-regulated. These differentially expressed
spots can be isolated and sequenced for identifying protein subunits
affected by salt stress.