Elucidation of rRNA secondary structure and phylogenetic analysis of plant growth promoting Streptomyces sp. based on 16 s RNA

Abstract

An increasing demand for low-input agriculture has resulted in a greater interest in soil microorganisms which are able to enhance plant nutrition and health, and to improve soil quality. Actinomycetes represent a high proportion of the soil microbial biomass and have the capacity to produce a wide variety of antibiotics and extracellular enzymes. Several strains of actinomycetes have also been found to be possessing plant growth promoting potential and also able to protect plants against various diseases. In the present study, Streptomyces sp. was isolated from the wheat-sugarcane cropping system. The genomic DNA of Streptomyces sp.  was extracted for 16sRNA gene sequencing.  Phylogenetic analysis was also carried out. The 16sRNA gene sequence obtained was used to deduce the secondary structure of RNA. In-vitro screening of isolates for their plant growth promoting activities was also done and result revealed that Streptomyces sp. have the potential for phosphate solubilisation, ammonia production, IAA production, siderophore production, HCN production and zinc solubilisation.