Mitigation of Salt Stress in Chickpea (Cicer arietinum L.) Through Seed Priming
Mitigation of salt stress in chickpea
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Authors
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Basavarajeshwari Policepatil
Department of Seed Science and Technology, University of Agricultural Sciences, Raichur-584104, Karnataka, India
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SR Doddagoudar
Seed Unit, University of Agricultural Sciences, Raichur-584104, Karnataka, India
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Basave Gowda
Seed Unit, University of Agricultural Sciences, Raichur-584104, Karnataka, India
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IM Sangeeta
Department of Seed Science and Technology, University of Agricultural Sciences, Raichur-584104, Karnataka, India
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Lakshmikanth
Department of Soil Science and Agricultural chemistry, University of Agricultural Sciences, Raichur-584104, Karnataka, India
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Laxuman
Zonal Agricultural Research station (ZARS), Kalaburgi-585101, University of Agricultural Sciences, Raichur, Karnataka, India
Keywords:
Chickpea, Gibberellic acid, Salicylic acid, Salinity, Seed germination, Seed primingAbstract
A laboratory experiment to study the influence of seed priming on seed germination and seedling vigour of the saline sensitive chickpea genotype (JG 11) was carried out in a two factorial completely randomized design, with four replications. The seeds of salt sensitive chickpea genotype (JG 11) were primed with six different chemicals with two concentrations viz., hydro priming, gibberellic acid (GA3) (100 and 150 ppm), salicylic acid (50 and 100 µM), sodium nitroprusside (SNP) (50 and 100 μM), KNO3 (0.5 and 1 %), K2HPO4 (0.5 and 1%), thiourea (500 and 1000 ppm) for 8 hours with 1:2 seed to solution ratio (weight by volume, w/v). The primed seeds were subjected for germination test by following between paper method by soaking in distilled water (0 mM NaCl-control-S1) and 60 mM NaCl (S1). Among the different treatments, imposed seed priming with salicylic acid (100 µM) significantly increased the seed germination (96.5 %), peroxidase activity (2.41 µmol ml-1 min-1), catalase activity (0.48 µmol ml-1 min-1) and proline content (3.50 µmol g-1 FW) while GA3 (150 ppm) significantly increased the root length (16.3 cm), shoot length (17.5 cm) and seedling vigour index-I (3807) compared to all other priming treatments and control.
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