Effect of Steam Sterilization on Soil Biological and Chemical Properties
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Authors
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Madhu Choudhary
ICAR-Central Soil Salinity Research Institute (CSSRI), Karnal, Haryana, India
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Savita Kajal
Department of Environmental Sciences, Kurukshetra University, Kurukshetra, Haryana, India
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HS Jat
ICAR-Central Soil Salinity Research Institute (CSSRI), Karnal, Haryana, India
Keywords:
Autoclaving, Sterilization, Micro-organisms, Soil biologyAbstract
Sterilization is commonly used in the studies related to soil biological experiments. Among different methods of soil sterilization steam sterilization is widely used method which is also called “autoclaving” and is most convenient, cheap and fast method. However, there is lack of information about how steam sterilization is effective in the elimination of microbes especially heat resistant. It is also not very much known how different soils behave after different types of steam sterilization. Therefore, we conducted an experiment with normal, saline and sodic soils and tested how effectiveness of steam sterilization can be improved by repeated autoclaving. Additionally, we also checked whether steam sterilization has any effect on soil properties like soil electric conductivity (EC), pH, microbial populations and nutrients and how these properties responded after multiple sterilizations. It was found that for all three types of soils EC was decreased while pH increase in normal and saline soils with autoclaving. Soil nutrients behave differently in different types of soils. Bacterial and fungal populations were found highest in normal soil. Slow growing fungi were found to more sensitive towards sterilization than fast growing fungi and bacteria. Single time autoclaving is not sufficient for the elimination of microbes so it is recommended to sterilize soil two or more times
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References
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Journal of Soil Salinity and Water Quality serves as an official organ of the Indian Society of Soil Salinity and Water Quality (ISSSWQ) for the publication of research papers, reviews, and short communications as per the constitution and by-laws of the society. Soft and hard copy of the journal are sent free to all its members. All disputes are subject to the exclusive jurisdiction of competent court and forums in Kamal only. The society does not assume any responsibility for opinion by the authors in the articles and no-material in any form can be reproduced without the prior permission of the society. The society is not responsible for any delay, whatsoever, in publication/ delivery of the periodicals to the subscribers due to unforeseen circumstances or postal delay. The society does not vouch for any claims made by the advertisers of products and services. The publisher and the editors shall not be held liable for any consequences in the event of such claim not being honoured by the advertisers.
