IN-VITRO DETACHED LEAF ASSAY OF HOST-MEDIATED RNAi LINES CARRYING PHYTOPHTHORA INFESTANS AVR3A EFFECTOR GENE FOR LATE BLIGHT RESISTANCE

Authors

  • Suman Sanju
  • Aditi Thakur
  • Sundaresha Siddappa
  • Sanjeev Sharma
  • Pradeep Kumar Shukla
  • Nidhi Srivastava
  • Debasis Pattanayak
  • B P Singh

Keywords:

amiRNA, Avr3a, effector, gene silencing, Phytophthora infestans, potato, siRNA

Abstract

Phytophthora infestans, the causal organism of potato late blight adversely affects the quality as well as quantity of the potato crop. Late blight is the most dreaded biotic stress of potato till date. Although, a number of management strategies have been developed for the effective management of the disease but the fast evolving virulent strains of P. infestans still pose a challenge. The RXLR effector gene Avr3a from P. infestans has been silenced through small interfering RNA (siRNA) and artificial micro RNA (amiRNA) techniques, as Avr3a has been demonstrated to be essential factor for virulence in P. infestans. Phenotyping of Kufri Khyati and Kufri Pukhraj lines of potato developed through siRNA and amiRNA for late blight resistance was undertaken using detached leaf assay. The siRNA derived lines Kufri Khyati 1037 and Kufri Khyati 1129 and amiRNA derived line Kufri Khyati 2.1358 exhibited less lesion area as well as spore production and categorized as moderately resistant. Also, the reduced expression of Avr3a transcript in the siRNA derived lines Kufri Khyati 1037 and Kufri Khyati 1129 and amiRNA derived line Kufri Khyati 2.1358 coincided with the less disease severity in the leaves of transformed plants. Although both techniques of RNAi had remarkable effect on the pathogenicity of the gene Avr3a still siRNA mediated technique of silencing performed better than amiRNA mediated gene silencing when tested through detached leaf assay.

Downloads

Download data is not yet available.

Author Biographies

Suman Sanju

ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh, India

Aditi Thakur

ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh, India

Sundaresha Siddappa

ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh, India

Sanjeev Sharma

ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh, India

Pradeep Kumar Shukla

Sam Higginbottom Institute of Agriculture, School of Biological Sciences, Allahabad-211007, Uttar Pradesh, India

Nidhi Srivastava

Banasthali University, Rajasthan-304022, India

Debasis Pattanayak

ICAR-National Research Centre for Plant Biotechnology, IARI, New Delhi 110012, India

B P Singh

ICAR-Central Potato Research Institute, Shimla-171001, Himachal Pradesh, India

Downloads

How to Cite

Sanju, S., Thakur, A., Siddappa, S., Sharma, S., Shukla, P. K., Srivastava, N., Pattanayak, D., & Singh, B. P. (2016). IN-VITRO DETACHED LEAF ASSAY OF HOST-MEDIATED RNAi LINES CARRYING PHYTOPHTHORA INFESTANS AVR3A EFFECTOR GENE FOR LATE BLIGHT RESISTANCE. Potato Journal, 43(1). Retrieved from https://epubs.icar.org.in/index.php/PotatoJ/article/view/59702

Issue

Section

Articles