EFFECT OF CULTURE MEDIA ON GROWTH AND SPORULATION OF Colletotrichum lindemuthianum (SACC AND MAGN) ISOLATES CAUSING ANTHRACNOSE OF FIELD BEAN


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Authors

  • S. NARASIMHA RAO* , S.L. BHATTIPROLU AND V. PRASANNA KUMARI Department of Plant Pathology, College of Horticulture, Dr. YSR Horticultural Univeristy, V.R. Gudem – 534 101

https://doi.org/10.58537/jorangrau.2023.51.4.08

Keywords:

Colletotrichum lindemuthianum, Culture media, Field bean, Radial growth, Sporulation

Abstract

Anthracnose (Colletotrichum lindemuthianum) is the most important fungal disease affecting field bean. Growth and sporulation of thirty six isolates of C. lindemuthianum collected from five agro-climatic zones of Andhra Pradesh was evaluated in the year 2017-18 on four different solid and liquid culture media. Results revealed that potato dextrose agar was the best for both radial growth (66.74 mm) and dry mycelial weight (264.74 mg), whereas, host leaf extract agar significantly supported more sporulation 7.3 x104. The least mycelia growth of 39.32 mm, dry mycelial weight of 234.70 mg and sporulation 2.9 x104 were observed on Sabourband’s agar. Among the different isolates, Cl-33 grew fast with mycelial growth and sporulation (78.86 mm and 8.4x104) and Cl-32 produced the highest dry mycelial weight 266.75 mg). The least radial growth (31.35 mm), the lowest sporulation (4.1 x104) and lowest mycelial dry weight (227.75 mg) was recorded in CI-28, CI-1 and CI 17, respectively. Mean conidial length of 36 isolates ranged from 11.87 to 13.03 µm with the maximum length (13.03 µm) recorded in host leaf extract agar, while minimum was recorded in oat meal agar. Width of conidia varied from 3.59 to 4.11 µm on different media and 3.53 to 4.23 µm in different isolates.The growth phase study revealed that increase in the dry mycelial weight of fungus upto 15 days of incubation and growth declined from 17th day onwards.

References

Asalkar, U.A., Hingole, D.G.,Khaire, P. Band

Mete, V.S. 2019. Effect of different

solid media on the growth and

sporulation of Colletotrichum

gloeosporioides Penz. and Sacc.

causing fruit rot of Aonla. International

Journal of Current Microbiology and

Applied Sciences. 8 (10): 610-616.

Baxter, A. P., Van der Westhuizen, G.C.A and

Eicker, A. 1983. Morphology and

taxonomy of South African isolates of

Colletotrichum. South African Journal of

Botany. 2: 259-289.

Chaudhary, K and Singh, R. 2016. Influence

of relative humidity and media on growth

Figure 1. Growth of isolate CI 4 on different solid media after 15 days of incubation

NARASIMHA RAO et al.

and sporulation of Colletotrichum capsici

f. sp. cyamopsicola causing anthracnose

of cluster bean. International Journal of

Science and Research. 7 (5): 11071-

Dhingra, O.D and Sinclair, J.B. 2012. Basic

Plant Pathology Methods. 2nd Edition.

Lewis Publishers.

Denobys, B and Baudry, A. 1995. Species

identification and pathogenicity strictly of

France Colletotrichum strains isolated

from strawberry using morphological and

cultural characteristics. Phytopathology.

: 53-57.

Ismunadji, Mand Arsyad, D.M. 1990. L ablab

bean: An unexploited potential food

legume. Paper presented to training/

workshop meeting held in Bogor,

Indonesia, on improvement of

unexploited and food potential legumes

in Asia, 27th October to 3rd November,

Krishna, K., Bhagati,S., Madhuri, K., Amaresan,

N and Srivastava, R.C. 2011. Variability

in Colletotrichum gloeosporioides from

Indian Bay Island Ecosystem.

IndianPhytopathology. 64 (2): 189-191.

Kumar, A., Singh, T.K., Prashant Kumar, Bose,

U.S and Tiwari, R.K. 2017. Pathological

and cultural variability in Colletotrichum

gloeosporioides (Penz. &Sacc.) inciting

anthracnose of mango. International

Journal of Current Microbiology and

Applied Sciences. 6 (10): 2543-2550.

Kumar, S., Singh, V and Garg, R. 2015.

Cultural and morphological variability in

Colletotrichum capsici causing

anthracnose disease. International

Journal of Current Microbiology and

Applied Science. 4 (2): 243-250.

Mendes-Costa, M.C.1996. Genetics of

Glomerella cingulata f.sp. phaseoli I.

Sexual compatibility. Revista Brasileira

de Genética, Ribeirão Preto.

Suplemento do Congresso Nacional de

Genética, Caxambu. pp. 42.

Mihailovic, V, Mikic, A., Vasic, M., Cupina, B,

Duric, B.,DucG.,Stoddard, F.L and

Hauptvogel, P. 2010. Neglected legume

crops of Serbia-Fababean (Vicia faba).

Ratarstvoipovrtarstvo. 47: 27-32.

Murphy,A.M and Colucci, P.E. 1999. A tropical

forage solution to poor quality

ruminant diets: a review of Lablab

purpureus. Livestock Research and

Rural Development. 11: 2-16.

Pereira, J.C.R., Batista, U.G., Gulmaraes, F.B

and Misubuti, E.S.B. 1998. Effect of

different culture media on the sporulation

and inoculum potential of Colletotrichum

Iindemuthianum .Summa

Phytopathologica. 24(2): 186-189.

Pria, M.D., Bergamin Filho, A and Amorin, L.

Evaluation of different culture

media for sporulation of Colletotrichum.

Summa Phytopathologica. 23(2): 181-

Rajesha, G and Mantur, S.G. 2014. Studies on

morphological and cultural characters of

Colletotrichum lindemuthianum inciting

anthracnose of Dolichos bean. Journal

of Mycopathological Research. 52 (1):

-124.

Sanjeev Leharwan, Malik, V.K., Rajender

Singh and Munish Leharwan. 2018.

Effect of culture media and temperature

on growth and sporulation

Colletotrichum gloeosporioides cause

of anthracnose disease of mango.

International Journal of Current

EFFECT OF CULTURE MEDIA ON GROWTH AND SPORULATION OF Colletotrichum lindemuthianum ISOLATES

Microbiology and Applied Sciences. 7

(12): 1835-1842.

Sardhara, M. J., Davara, D. K., Moradia, A. M

and Kapadiya, H. J. 2016. Effect of

culture media and temperature on

growth and sporulation of Colletotrichum

lindemuthianum of urd bean in vitro.

International Journal of Plant Protection.

(1): 47 – 51.

Sharma, P. N., Sharma, O. P. Padder, B. A and

Kapil, R. 2008. Yield loss assessment in

kindney bean due to bean anthracnose

(Colletotrichum lindemuthianum) under

sub-temperate conditions of north

western Himalayas. Indian

Phytopathology. 61(3): 323-330.

Siva Prasad, B.M.V., Bhattiprolu, S.L., Manoj

Kumar, V., Jayalalitha, K and Sai Ram

Kumar, D.V. 2021. Cultural and

morphological variability of Corynespora

cassiicola (berk. and curt.) wei. causing

target spot disease in cotton.The Journal

of Research ANGRAU. 49 (2): 35-42.

Sonali Bhagat, Sing, S.K., Sneha Choudhary,

Ranbir Singh, Singh, V.B., Singh A.K

and Amrish Vaid. 2022. Studies on the

effect of different media on the mycelial

growth of Colletotrichum

lindemuthianum. Biological Forum – An

International Journal. 14 (4): 143-145.

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Submitted

20-02-2024

Published

26-03-2024

How to Cite

S. NARASIMHA RAO* , S.L. BHATTIPROLU AND V. PRASANNA KUMARI. (2024). EFFECT OF CULTURE MEDIA ON GROWTH AND SPORULATION OF Colletotrichum lindemuthianum (SACC AND MAGN) ISOLATES CAUSING ANTHRACNOSE OF FIELD BEAN. The Journal of Research ANGRAU, 51(4), 63-74. https://doi.org/10.58537/jorangrau.2023.51.4.08

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