MOLECULAR IDENTIFICATION OF NOVEL PROBIOTIC BACTERIA ISOLATED FROM COW MILK AND ITS ANTIBACTERIAL APPLICATIONS
389 / 101
Authors
-
BABINA CHANU KHOMDRAM* and A.THIRUMANI DEVI
Food Science and Nutrition, School of Home science Avinashilingam Institute for Home Science and Higher Education for Women Coimbatore- 641 043
Keywords:
Antibacterial, Isolate, Limosilactobacillus fermentum, Lactobacillus sp, LAB, Probiotic.Abstract
The purpose of the study conducted in the year 2021 was to isolate and identify Lactobacillus species through biochemical characterization and to find antibacterial potential of the species. Researchers collected milk sample from local milk vendor, Eachanari in December, 2021. In an Enriching MRS broth the colonies were isolated and loop full of isolate were streaked on MRS agar plate. By morphological characteristics determined through gram staining method the isolated bacteria were identified as Lactobacillus species. Through different identification methods such
as casein digestion, acid and salt tolerance the isolate was characterized for probiotic properties. In a skim milk agar plate the isolated strain digested casein. This indicated production of protease enzyme which is a significant probiotic property. In MRS broth, an acid tolerance test was conducted at pH levels 3, 4, 5, 6, 7 and 8. The isolate was able to endure both an extremely acidic pH and alkaline media. NaCl tolerance test was done in MRS broth at concentrations of 3%, 4%, 5%, 6%, 7%, and 8% NaCl. The isolate survived well in solutions containing 3%, 4%, and 6% NaCl. After biochemical studies, molecular identification was carried out to identify the probiotic starin. The isolated probiotic was identified as Limosilactobacillus fermentum strain BTFSN. The isolated strain was then checked for antibacterial activity against three pathogens. Zone of inhibition were observed. The study indicated that Lactobacillus species from milk sample have potential probiotic properties and possess antimicrobial property.
References
Abd El Hack, M. E., El Saadony, M. T., Shafi,
M. E., Qattan, S. Y., Batiha, G. E.,
Khafaga, A. F and Alagawany, M. 2020.
Probiotics in poultry feed: A
comprehensive review. Journal of Animal
Physiology And Animal Nutrition. 104(6):
-1850.
Abd El-Lateef, H. M., Abdel-Rahman, E. S and
Mohran, H. S. 2015. Role of Ni content
in improvement of corrosion resistance
of Zn–Ni alloy in 3.5% NaCl solution.
Transactions of Nonferrous Metals
Society of China, 25(8): 2807-2816.
Ahmed, A. S. I., El Moghazy, G. M., Elsayed, T.
R., Goda, H. A. L and Khalafalla, G. M.
Molecular identification and in vitro
evaluation of probiotic functional
properties of some Egyptian lactic acid
bacteria and yeasts. Journal of Genetic
Engineering and Biotechnology. 19(1):
-16.
Atanasova, J., Moncheva, P and Ivanova, I.
Proteolytic and antimicrobial
activity of lactic acid bacteria grown in
goat milk. Biotechnology and
Biotechnological Equipment. 28(6):
-1078.
Bustos, A. Y., de Valdez, G. F., Fadda, S and
Taranto, M. P. 2018. New insights into
bacterial bile resistance mechanisms:
the role of bile salt hydrolase and its
impact on human health. Food Research
International. 112: 250-262.
Dj, B. 1988. Elie Metchnikoff’s bacillus of long
life. ASM news. 54: 661-665.
Figure 11. This graph reflects the inhibitory zone
BABINA CHANU and THIRUMANI DEVI
Fuller, R. 1989. Probiotics in man and animals.
The Journal of Applied Bacteriology.
(5): 365-378.
Hassan, A. A., Radwan, H. A., Abdelaal, S. A.,
Al-Radadi, N. S., Ahmed, M. K., Shoueir,
K. R and Hady, M. A. 2021.
Polycaprolactone based electrospun
matrices loaded with Ag/hydroxyapatite
as wound dressings: Morphology, cell
adhesion, and antibacterial activity.
International Journal Of Pharmaceutics.
: 120143.
Kumar, R., Dwivedi, V., Nayyar, N., Verma, H.,
Singh, A. K., Rani, P and Lal, R. 2015.
Parapedobacter indicus sp. nov.,
isolated from hexachlorocyclohexanecontaminated soil. International Journal
of Systematic and Evolutionary
Microbiology. 65: 129-134.
Lin, X., Zhang, T., Ju, L., Jiang, Y., Hou, Q.,
Hu, Z and Wang, Z. 2021. Effects of
supplemental feeding of probiotics
during pactation on rumen microflora of
calves after weaning. Advances in
Bioscience and Biotechnology. 12(7):
-228.
Lilly, D. M and Stillwell, R. H. 1965. Probiotics: DOI: https://doi.org/10.1002/j.2333-8504.1965.tb00518.x
growth-promoting factors produced by
microorganisms. Science. 147(3659):
-748.
Mannan, S. J., Rezwan, R., Rahman, M. S and
Begum, K. 2017. Isolation and
biochemical characterization of
Lactobacillus species from yogurt and
cheese samples in Dhaka metropolitan
area. Bangladesh Pharmaceutical
Journal. 20(1): 27-33.
Mokoena, M. P. 2017. Lactic acid bacteria and
their bacteriocins: classification,
biosynthesis and applications against
uropathogens: a mini-review. Molecules.
(8).1255.Doi.org/10.3390/
molecules22081255
Pundir, R. K., Kashyap, S. R. N., and Kaur, A.
Probiotic potential of lactic acid
bacteria isolated from food samples: an
in vitro study. Journal of Applied
Pharmaceutical Science. 3(3): 085-093.
Rao, X. J, Shahzad, T, Liu, S., Wu, P, He, Y. T,
Sun, W. J and Yu, X. Q. 2015. DOI: https://doi.org/10.1155/2015/853461
Identification of C-type lectin-domain
proteins (CTLDPs) in silkworm Bombyx
mori. Developmental & Comparative
Immunology. 53(2): 328-338.
Rodrigues, F. J., Cedran, M. F., Bicas, J. L and
Sato, H. H. 2020. Encapsulated probiotic
cells: Relevant techniques, natural
sources as encapsulating materials and
food applications– A narrative review.
Food Research International. 137:
Silva, D. R, Rosalen, P. L., Freires, I. A, Sardi,
J. D. C. O., Lima, R. F., Lazarini, J. G.
and Costa, E. M. M. D. B. 2019.
Anadenanthera Colubrina vell Brenan:
anti-Candida and antibiofilm activities,
toxicity and therapeutical action. Brazilian
Oral Research. 33. doi.org/10.1590/
-3107bor-2019.vol33.0023
Tachedjian, G., Aldunate, M., Bradshaw, C. S
and Cone, R. A. 2017. The role of lactic
acid production by probiotic Lactobacillus
species in vaginal health. Research in
Microbiology. 168(9-10): 782-792.
Tuncer, B. O and Tuncer, Y. 2014.
Exopolysaccharide producer
Streptococcus thermophilus ST8. 01
strain. A potential probiotic culture. GIDA.
(4): 195-202.
Ubeda, C and Pamer, E. G. 2012. Antibiotics,
microbiota, and immune defense. Trends
in Immunology. 33(9): 459-466.
MOLECULAR IDENTIFICATION OF NOVEL PROBIOTIC BACTERIA ISOLATED FROM COW MILK
Verlet, E, Bouhous, Houque, D, Messaoudi, L,
Houyengah, F and Lawniczak, A. J. 2010.
P13 Enquête «Diabète un jour» au sein
d’un Centre Hospitalier de 486 lits:
résultats d’une démarche Qualité.
Diabetes & Metabolism. 36, A43.
Xiang, H, Sun-Waterhouse, D, Waterhouse, G.
I, Cui, C, and Ruan, Z. 2019.
Fermentation-enabled wellness foods: A
fresh perspective. Food Science and
Human Wellness. 8(3): 203-243.
Yang, H, Sun, Y, Cai, R, Chen, Y, and Gu, B.
The impact of dietary fiber and
probiotics in infectious diseases.
Microbial Pathogenesis. 140: 103931.
Zhao, F., Ma, F., Shi, R., Zhang, J., Han, S and
Zhang, Y. 2015. Production of
rhamnolipids by Pseudomonas
aeruginosa is inhibited by H 2 S but
resumes in a co-culture with P. stutzeri:
applications for microbial enhanced oil
recovery. Biotechnology letters. 37:
-1808.
Downloads
Submitted
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The author owns the article's copyright until the article is accepted for publication. After acceptance, the author(s) assigns the article's copyright jointly to both the authors and the Publishers of the Journal of Research ANGRAU (ANGRAU) and licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License.
