ENHANCED LIPASE PRODUCTION FROM CELOSIA ARGENTEA SEED OIL USING MUTANT BACILLUS PUMILUS UVM6
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Authors
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P. SATHIYA
PG & Research Department of Botany Arignar Anna Govt. Arts College (Affiliated to Thiruvalluvar University Vellore), Cheyyar, Tamil Nadu 604 407, India
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K. DHANDAYUTHAPANI
PG & Research Department of Botany Arignar Anna Govt. Arts College (Affiliated to Thiruvalluvar University Vellore), Cheyyar, Tamil Nadu 604 407, India
Keywords:
Bacillus pumilus, Celosia argentea seed oil, lipase, mutation, submerged fermentationAbstract
A mutant strain was developed by random mutation using UV-C radiation from wild parent strain Bacillus pumilus KMAS7. Following mutagenesis, eleven bacterial isolates were used for maximum lipase production and found that the strain UVM6 produced high lipase of 36.46±0.05 UmL-1 from C. argentea seed oil. It was 1.38 times more abundant than the wild parent strain. Robust mutant strain UVM6 was selected and designated as B. pumilus UVM6. Then this mutant strain was cultivated in SmF for production of lipase using C. argentea seed oil under different nutritional sources. An overall 2.95-fold enhanced extracellular lipase production (62.42±0.03 UmL-1) was obtained from C. argentea seed oil by SmF using 4% v/v C. argentea seed oil, 0.5% w/v glucose, 1% w/v ammonium sulphate and 10 μML-1 Zn as inducer, carbon, nitrogen and trace element sources respectively. Seed oil of C. argentea has been used in traditional folk medicine, but this is the first time we’re reporting lipase production from C. argentea by the newly isolated mutant B. pumilus UVM6.
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