CHARACTERIZATION OF ARSENIC TOLERANT RHIZOBIUM Sp. ISOLATED FROM ROOT NODULES OF VIGNA RADIATA
348 / 53
Authors
-
P. VEERABRAMHACHARI
Department of Biotechnology, Krishna University, Machilipatnam-521 004- AP.
-
E. KARIALI
Department of Biotechnology, Krishna University, Machilipatnam-521 004- AP.
Keywords:
Rhizobium identificaton, Vignaradiata, Arsenic tolerance, cellulase, amylaseAbstract
The rationale of the study was to characterize arsenic tolerant Rhizobiumsp. from root nodules of Vigna radiate in the vicinity of the fertilizer production plant contaminated with arsenic. The current investigation involved the interactions of Rhizobium sp. in remediating arsenic has been studied by isolating root nodules of Mung Bean (Vigna radiata) and analyzed for strain characterization, CFU/mL counts, biomass yield, enzymes (cellulase and amylase), and proteins. The screened bacteria were shown to be both arsenic tolerant and sensitive. All isolates were determined to be Rhizobium sp. based on colony, morphology, biochemistry, and nodulation ability analysis. The arsenic sensitivity of the chosen isolates was confirmed by low CFU/mL counts and low biomass output in the medium. The results showed that strain MRR121 is sensitive to arsenic, while strain VBCK1062 showed significant tolerance levels based on the arsenic sensitivity tests. The results emphasized that the sensitivity and tolerance of Rhizobium sp. isolates to arsenate was determined, with reference to their growth and biomass yields, enzyme assays, and protein profiles. The isolated Rhizobium strains exhibited growth retardation when the As concentration was raised beyond 25 mg/mL, as confirmed by the CFU and biomass. The study has clearly evidenced that while increased amounts of arsenic are extremely harmful to the Rhizobium strain The As tolerant strain VBCK1062, outproduced the sensitive strain in terms of proteins and enzymes (cellulase and amylase). Based on the computed similarity coefficient, there is a 76% similarity between the tolerant strain and the commercial (MTCC 616) strains. This study gives better understanding of plant-metal-microbe interactions and hence can be used as a criterion for isolation of As (V) tolerant Rhizobium sp. from metal contaminated agro-geo-ecosystems.
References
1. Ahmad, I., Hayat, S., Ahmad, A., Inam, A and
2. Samiullah. 2001. Metal and antibiotic
3. resistance traits in Bradyrhizobium
4. sp.(cajanus) isolated from soil receiving
5. oil refinery wastewater. World Journal of
6. Microbiology and Biotechnology, 17:
7. -384.
8. Carbonell-Barrachina, A. A., Aarabi, M. A.,
9. DeLaune, R. D., Gambrell, R. P and
10. Patrick, W. H. 1998. The influence of
11. arsenic chemical form and concentration
12. on Spartina patens and Spartina
13. alterniflora growth and tissue arsenic
14. concentration. Plant and Soil, 198: 33-
15. Gauri Singh, A.K., Bhatt, R.P., Pant, S and Bedi,
16. M. 2010. Effect of Arsenic on nodulation
17. and growth parameters of Vigna radiata.
18. Crop Res. 40(1,2&3): 186-191.
19. Gianfreda, L., Rao, M.A.,Piotrowska, A.,
20. Palumbo, G and Colombo, C. 2005. Soil
21. enzymes activities as affected by
22. anthropogenic alterations: intensive
23. agricultural practices and organic
24. pollution. Science of Total Environ: 341:
25. -279.
26. Hunter, W.J., Kuykendall, L.D and Manter, D.K.
27. Rhizobium selenireducens sp. nov.
28. A Selenite-Reducing - Proteobacteria
29. Isolated From a Bioreactor. Curr.
30. Microbiol. 55: 455-460.
31. Kabata-Pendias, A. 1992. Trace Elements in
32. Soils and Plants, 3rd ed.; CRC Press:
33. Boca Raton, FL, USA.
34. Lowry, D.H., Rosebrough, N.J., Farr, A.L and
35. Randall, R.J. 1951. Protein determination
36. of the Lowry Assay. J. Biol. Chem. 193:
37. -275.
38. Mandal, S.M., Pati, B.R., Das, A.K and Ghosh,
39. A.K. 2008. Characterization of arsenic
40. tolerant and nodulation effective
41. Rhizobium isolate of V. mungo. J. Gen.
42. Appl. Microbiol. 54: 93–99.
43. Robinson, B., Russell, C., Hedley, M and
44. Clothier, B. 2001. Cadmium adsorption
45. by rhizobacteria: implications for New
46. Zealand pastureland. Agri. Eco. Environ.
47. : 315–321.
48. Sofia, Isabel., Almeida, Pereira., Ana, Isabel.,
49. Gusmão, Lima., Etelvina Maria, de
50. Almeida and Paula, Figueira. (2006).
51. Heavy metal toxicity in Rhizobium
52. leguminosarum biovarviciae Isolated from
53. soils subjected to different sources of
54. heavy-metal contamination: Effects on
55. protein expression. App. Soil. Ecol.
56. :286-293.
57. Valls, M and de Lorenzo, V. 2002. Exploiting
58. the genetic and biochemical capacities
59. of bacteria for the remediation of heavy
60. metal pollution. FEMS Microbiol. Rev. 26:
61. –338.
62. Wei, G.H., Tan, E.T., Zhu, M.E., Wang, E.T.,
63. Han,S.Z and Chen, W.X. 2003.
64. Characterization of rhizobia isolated from
65. legume species within the genera
66. Astragalus and Lespedeza grown in the
67. Loess Plateau of China and description
68. of Rhizobium loessense sp. Int. J. Evol.
69. Microbiol. 53:1575-1583.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The author owns the article's copyright until the article is accepted for publication. After acceptance, the author(s) assigns the article's copyright jointly to both the authors and the Publishers of the Journal of Research ANGRAU (ANGRAU) and licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License.
