DETECTION OF CRY1AC AND CRY2AB PROTEINS WITH ELISA AND PCR TECHNIQUES IN TRANSGENIC COTTON (GOSSYPIUM HIRSUTUM L.)
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Authors
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RANI CHAPARA
Department of Genetics & Plant Breeding, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur – 522034
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N. CHAMUNDESWARI
Department of Genetics & Plant Breeding, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur – 522034
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L. RAJESH CHOWDHARY
Department of Genetics & Plant Breeding, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur – 522034
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CH. NAGAJYOTHI
Department of Genetics & Plant Breeding, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur – 522034.
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B. SREEKANTH
Department of Genetics & Plant Breeding, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur – 522034
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V. MANOJ KUMAR
Department of Genetics & Plant Breeding, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur – 522034
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S. RAJAMANI
Department of Genetics & Plant Breeding, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur – 522034.
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M. JAMES
Department of Genetics & Plant Breeding, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Lam, Guntur – 522034
Keywords:
Cotton (G.hirsutum L.), cry1Ac, cry2Ab, ELISA, PCR and PrimerAbstract
To develop BGII hybrids/varieties, the elite lines which were proven for high yield with jassid resistance were selected and converted under BGII background for further usage in BGII hybrid development programme. Hence, the study was aimed to detect the presence of Cry1Ac and Cry2Ab proteins/genes through qualitative ELISA, PCR techniques and to select the
homozygous population from the segregating generations consisting of 1663 samples from different segregating generations as well as from stabilized populations from OYT/PYT/AYT yield trials. The study revealed that out of 9125 samples, 8760 (96%) of samples showed the presence of cry 1Ac and cry 2Ab proteins in the transgenic leaf samples and also demonstrated that the technique of ELISA for identification of cry 1Ac and cry 2Ab proteins is quite handy and easily adoptable. Using PCR technique, the zygosity status of the samples were known for cry1Ac and cry2Ab genes; used for selection and advancement to next generations in the breeding programme.
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