CULTURAL AND MORPHOLOGICAL CHARACTERIZATION OF ALTERNARIA BRASSICAE OF MUSTARD IN ALLAHABAD UTTAR PRADESH
332 / 291
Authors
-
SHAILENDRA KUMAR
Department of Botany, Kutir P.G. College, Chakkey, Jaunpur -222146
-
S. M. PRASAD
Department of Botany, Kutir P.G. College, Chakkey, Jaunpur -222146
-
H. K. KEHRI
Department of Botany, Kutir P.G. College, Chakkey, Jaunpur -222146
Keywords:
Alternaria brassicae, cultural growth, leaf spot diseases, morphological variations, mustard crop, Allahabad mustard cropAbstract
Alternaria brassicae isolates were collected from the black leaf spot of mustard during the winter season (2019-2021) in the various regions of the Allahabad district. In vitro, results show significant variability in growth pattern, sporulation, conidial length, width, and septa number in different culture media, temperature, pH, and relative humidity. The conidial length of the isolates II-2C20, III-5C20, V-2A20, V-7A20, and I-6A1y21 was the shortest, measuring 14 ìm. The isolate I-4B21 and II-7C21 had the longest conidial length, ranging from 72 ìm to 72.8 ìm, respectively. The average conidial length ranged from 30.1 ìm to 50.9 ìm, with mustard isolate I-6A1y21 having the lowest value and I-4B21 having the highest. The highest average width was 15.86 ìm. The best mediums for 31 isolates were Potato Dextrose Agar, Meal Agar, Corn Meal Agar, Czapex dox agar, Carrot meal agar, and V-8 juice Agar. The highest radial growth was shown in the OMA medium by I-6A1y21 isolates, while the lowest growth was shown in the CA medium by II-7C21 and II-8B21 isolates. Out of all of them, the isolate I-6B121 sporulated the most (41.75x105/ml) whereas the isolate I-4A21 sporulated the least (0.5x105/ml) in OMA and CA medium. The radial growth and sporulation are optimal at 24°C, moderate at 18°C and 30°C, and lowest at 6°C and 36°C. Isolate III-3B20 and II-5C20 showed the least average radial growth and sporulation at all pH levels, while isolate I-6A1y21 showed maximum growth. The pH 7 is more favourable for radial growth and sporulation. The study found that radial growth and sporulation increased in relative humidity between 80-100%, while decreased in 20-40%, with isolate I-6A1y21 showing the highest growth while II-UN120 and III-5C21 exhibited the least. Among the 31 isolates, 9 isolates II-UN120, IV-5C20 I-4A21, I-6B21, I-6A1y21, II-8B21, III-6C21, IV-4B21, and IV- 6AY21 were found to be high degree of infection as the spot produced by them were more than 10 mm in diameter. Statistical analyses were conducted using Microsoft 365 Excel and SPSS 28.0 version on Windows 10, using ANOVA and Duncan’s multiple range test of homogeneity at a P=0.05 level.
References
1. Blagojevic, J. D., Vukojevic, J. B., and Ivanovic,
2. Ž. S. (2020). Occurrence and
3. characterization of Alternaria species
4. associated with leaf spot disease in
5. rapeseed in Serbia. Plant pathology,
6. 69(5), 883-900.
7. Chattopadhyay, C. (2008). Management of
8. diseases of rapeseed mustard with
9. special reference to Indian conditions.
10. (in) Sustainable Production of Oilseeds:
11. Rapeseed-Mustard Technology.
12. Agrotech Publishing Academy, Udaipur.
13. pp. 364–388.
14. 44
15. Shailendra Kumar et al.,
16. DRMR, (2020-2021). 28th Annual AIRCP (R&M)
17. Group Meeting, ICAR-Directorate of
18. Rapeseed-Mustard Research,
19. Bharatpur, Rajasthan, Pp 3-4.
20. Fatima, U., Bhorali, P., Borah, S., and Senthil
21. Kumar, M. (2019). Perspectives on the
22. utilization of resistance mechanisms from
23. host and nonhost plants for durable
24. protection of Brassica crops against
25. Alternaria blight. PeerJ, 7, e7486.
26. Fontaine, K., Fourrier-Jeandel, C., Armitage,
27. A. D., Boutigny, A. L., Crépet, M., Caffier,
28. V., Gnide, D. C., Shiller, J., Le Cam, B.,
29. Giraud, M., Ioos, R., and Aguayo, J.
30. (2021). Identification and pathogenicity
31. of Alternaria species associated with leaf
32. blotch disease and premature defoliation
33. in French apple orchards. PeerJ, 9,
34. e12496.
35. Goyal, P., Chahar, M., Mathur, A.P., Kuma, r A.
36. and Chattopadhyay, C. (2011).
37. Morphological and cultural variation in
38. different oilseed Brassica isolates of
39. Alternaria brassicae from different
40. geographical regions of India. Indian J.
41. Agric. Sci. 81 (11): 1052–1058.
42. Kumar, P., and Sharma, R.C. (2017).
43. “Comparative study of culture media for
44. the growth and sporulation of Alternaria
45. brassicae.” Indian Phytopathology, 70(3),
46. 421-427.
47. Pandey, M. K., Ahmad, M. M., Siddiqui, S.,
48. Kumar, A., and Singh, S. K (2023) a.
49. Alternaria Blight–A Serious Affliction of
50. Rapeseed Mustard: A Review.
51. Patel, S. N., Kumar, P., Raghuvanshi, V. V.,
52. Patel, S. K., Siddharth, P., Singh, A., and
53. Milan, A. (2023). Morphological
54. Characterization and Pathogenicity Test
55. of Alternaria Leaf Spot of Chinese
56. Cabbage (Brassica chinensis
57. L.). International Journal of Plant & Soil
58. Science, 35(19), 1616-1625.
59. Prasad, S., Kumar, R., and Singh, D.P. (2019). “Influence
60. of culture media on the growth and sporulation of
61. Alternaria brassicae.” Journal of Plant Pathology,
62. 101(2), 297-302.
63. Punia, R., Kumari, P., Kumar, A., Rathi, A. S.,
64. and Avtar, R. (2021). Impact of weather
65. parameters on alternaria blight of Indian
66. mustard [(Brassica juncea (L.) Czern. &
67. Coss.)]. Bangladesh Journal of
68. Botany, 50(1), 15-19.
69. Saha, S., Garg, R., Venkataravanappa, V.,
70. Mishra, P. K., Rai, A. B., and Singh, R. P.
71. (2016). Molecular and Cultural
72. Characterization of Alternaria brassicae
73. Infecting Cauliflower in Uttar Pradesh,
74. India. Proceedings of the National
75. Academy of Sciences, India Section B:
76. Biological Sciences, 86, 485-495.
77. Singh, H. K., Kumar, P., and Singh, S. K. (2021).
78. Yield loss assessment due to Alternaria
79. blight disease in rapeseed and mustard:
80. Yield loss due to alternaria Blight in
81. rapeseed and mustard. Journal of
82. AgriSearch, 8(3), 241-248.
83. Singh, J., Kumhar, D. R., Kumari, S., and Yadav,
84. A. L. (2021). Effect of physical
85. parameters on the sporulation and
86. mycelial growth of Alternaria brassicae
87. causing Alternaria leaf spot of
88. mustard. Plant
89. Disease
90. Research, 36(2), 184-187.
91. Thakur, O. (2024). Variability and management
92. of alternaria species causing leaf blight
93. of cauliflower (brassica oleracea l. var.
94. botrytis) (doctoral dissertation, dr.
95. yashwant singh parmar university of
96. horticulture and forestry).
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The author owns the article's copyright until the article is accepted for publication. After acceptance, the author(s) assigns the article's copyright jointly to both the authors and the Publishers of the Journal of Research ANGRAU (ANGRAU) and licensed under a Creative Commons Attribution-Non Commercial-Share Alike 4.0 International License.
