RAPID IN VITRO PROPAGATION OF ANANAS COMOSUS VAR. MICROSTACHYS USING CROWN EXPLANTS
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Authors
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SUCHETA GOLWALKAR
Department of Biotechnology, Ramniranjan Jhunjhunwala College of Arts, Science & Commerce (Empowered Autonomous), Ghatkopar (West), Mumbai-400086, Maharashtra, India
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ICHHA VIREN SHAH
Department of Biotechnology, Ramniranjan Jhunjhunwala College of Arts, Science & Commerce (Empowered Autonomous), Ghatkopar (West), Mumbai-400086, Maharashtra, India
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YASMIN KHAN
Department of Biotechnology, Ramniranjan Jhunjhunwala College of Arts, Science & Commerce (Empowered Autonomous), Ghatkopar (West), Mumbai-400086, Maharashtra, India
Keywords:
BAP, Crown, Dwarf pineapple (Ananas comosus var. microstachys), HardeningAbstract
The present study was conducted from December 2023 to August 2024 at the Department of Biotechnology, Ramniranjan Jhunjhunwala College, Mumbai, to develop an efficient in vitro propagation protocol for Ananas comosus var. microstachys. Crown explants were disinfected and cultured on Murashige and Skoog (MS) medium with varying concentrations of 6-benzylaminopurine (BAP) and kinetin (KN) (0.25 to 2.0 mg.L-¹) for shoot induction, and indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) (0.5 to 3.0 mg.L-¹) for rooting. The highest shoot proliferation was observed at 2.0 mg.L-¹ BAP, with 25 shoots/explant in the first subculture and 38 in the second. The maximum shoot length reached 5.5 cm. Rooting was optimal on full-strength MS medium supplemented with 1.0 mg.L-¹ IBA, yielding 10.67 roots/shoot with an average length of 7 cm. Primary hardening in a 1:1 cocopeat and vermiculite mixture resulted in 98% survival, while soil and sand (1:1) proved suitable for secondary hardening. The protocol ensures rapid, large-scale multiplication of dwarf pineapple for commercial floriculture applications.
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