GENOTYPIC VARIATION IN NORNICOTINE ACCUMULATION, A PRECURSOR TO HARMFUL CONSTITUTES OF TOBACCO
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Authors
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V KAVYA
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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Y AMARAVATHI
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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K PRABHAKARA RAO
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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P SUPRIYA
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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M SHESHUMADHAV
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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K SARALA
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, Indi
Keywords:
Nicotine, Nornicotine, TSNAsAbstract
The study aimed to estimate the content of nicotine and nornicotine levels across twenty
five tobacco genotypes,including FCV, Burley, HD-Burley, Bidi, Chewing, and Lanka types.
Chemical analysis showed significant differences for nicotine and nornicotine levels in 25 tobacco
genotypes.The highest nicotine content was recorded in FCR-15 (20.8 mg/g), and the lowest
nicotine content was observed in FCJ-11 (9.1 mg/g). Among 25 tobacco genotypes, nornicotine
content ranges from 7.74 mg/g (Bio Mut-55-1) to 0.0003 mg/g (LT Kanchan). Significant variation
was further observed in nicotine-to-nornicotine conversion rates.The Burley genotype Bio Mut
55- 1 exhibited the highest conversion rate (41.7%), followed by Banket A1 (8.453%). Importantly,
several genotypes showed negligible nornicotine accumulation. The genotypes with low
nornicotine accumulation provide valuable insight for breeding low-TSNA tobacco genotypes,
thereby improving the safety profile of tobacco products.
References
1. Elchamieh, S., Jaber, A., Ibrahim, G and
2. Cheble, E. 2024. Spectrophotometric
3. and Chromatographic Determination of
4. Alkaloids and Nicotine Contents in
5. Lebanese Tobacco Leaves. YuzuncuYýl
6. University Journal of Agricultural
7. Sciences, 34(2):335-345.
8. FAO STAT 2023-2024.
9. Hidalgo Martinez, D., Payyavula, R.S.,
10. Kudithipudi, C., Shen, Y., Xu, D., Warek,
11. U., Strickland, J.A and Melis, A. 2020.
12. Genetic attenuation of alkaloids and
13. nicotine content in tobacco (Nicotiana
14. tabacum). Planta, 251:1-14. https://
15. tobaccoboard.com 2022-2023 Tobacco
16. annual report.
17. Lewis, R.S., Jack, A.M., Morris, J.W., Robert,
18. V.J., Gavilano, L.B., Siminszky, B., Bush,
19. L.P., Hayes, A.J and Dewey, R.E. 2008.
20. RNA interference (RNAi) induced
21. suppression of nicotine demethylase
22. activity reduces levels of a key
23. carcinogen in cured tobacco leaves.
24. Plant Biotechnology Journal, 6(4):346
25. 354.
26. Moghbel, N., Ryu, B., Ratsch, A and Steadman,
27. K.J. 2017. Nicotine alkaloid levels, and
28. nicotine to nornicotine conversion, in
29. Australian Nicotiana species used as
30. chewing tobacco. Heliyon. 3(11).
31. Rodgman, A and Perfetti, T. A. 2009. Nicotine
32. and Tobacco Alkaloids with a Six-Member
33. N-containing ring and a second Five
34. Membered N-containing ring. The
35. chemical components of tobacco and
36. tobacco smoke, Rodgman A., Perfetti TA
37. (Eds), CRC press, Taylor and Francis,
38. Boca Raton Florida, 924-926.
39. 51
40. GENOTYPIC VARIATION IN NORNICOTINE ACCUMULATION IN TOBACCO
41. Shoji, T., Kajikawa, M and Hashimoto, T. 2010.
42. Clustered transcription factor genes
43. regulate nicotine biosynthesis in
44. tobacco. The Plant Cell, 22(10):3390
45. 3409.
46. Siminszky, B., Gavilano, L., Bowen, S.W. and
47. Dewey, R.E. 2005. Conversion of
48. nicotine to nornicotine in Nicotiana
49. tabacum is mediated by the CYP82E4,
50. a cytochrome P450 mono oxygenase.
51. Proceedings of the National Academy of
52. Sciences, 102(41) : 14919 - 14924.
53. Song, S., Wang, Y., Wang, J., Liu, Y., Zhang,
54. X., Yang, A and Li, F. 2024. Low
55. H3K27me3 deposition at CYP82E4
56. determines the nicotinic conversion rate
57. in Nicotiana tabacum. Plant Physiology
58. and Biochemistry, 206, p.108234.
59. Tassew, Z. and Chandravanshi, B.S. 2015.
60. Levels of nicotine in Ethiopian tobacco
61. leaves. SpringerPlus, 4:1-6.
62. Tayoub, G., Sulaiman, H and Alorfi, M. 2015.
63. Determination of nicotine levels in the
64. leaves of some Nicotiana tabacum
65. varieties cultivated in Syria.
66. HerbaPolonica, 61(4).
67. Wang, Y., Zhang, X., Zhang, F., Cheng, L.,
68. Jiang, C., Yang, A and Li, F. 2025. DNA
69. Methylation in CYP82E4 Regulates
70. Nicotine Conversion of Nicotiana
71. tabacum. Plant, Cell & Environment.
72. Wu, W., Ashley, D.L. and Watson, C.H., 2002.
73. Determination of nicotine and other
74. minor alkaloids in international cigarettes
75. by solid-phase micro-extraction and gas
76. chromatography / mass spectrometry.
77. Analytical Chemistry, 74(19) : 4878
78. 4884.
79. Xu, D., Shen, Y., Chappell, J., Cui, Mand
80. Nielsen, M.2007.Bio chemical and
81. molecular characterizations of nicotinede
82. methylase in tobacco. Physiologia
83. Plantarum, 129(2):307-319. Zenkner,
84. F.F., Margis-Pinheiro, M and Cagliari, A.
85. 2019. Nicotine biosynthesis in Nicotiana:
86. a metabolic overview. Tobacco Science,
87. 56(1):1-9
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