GENOTYPIC VARIATION IN NORNICOTINE ACCUMULATION, A PRECURSOR TO HARMFUL CONSTITUTES OF TOBACCO
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Authors
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V KAVYA
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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Y AMARAVATHI
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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K PRABHAKARA RAO
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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P SUPRIYA
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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M SHESHUMADHAV
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, India
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K SARALA
Dept. of Molecular Biology and Biotechnology, S.V. Agricultural College, Tirupati-517502 Acharya N.G. Ranga Agricultural University, Lam,Guntur, Andhra Pradesh, Indi
Keywords:
Nicotine, Nornicotine, TSNAsAbstract
The study aimed to estimate the content of nicotine and nornicotine levels across twenty
five tobacco genotypes,including FCV, Burley, HD-Burley, Bidi, Chewing, and Lanka types.
Chemical analysis showed significant differences for nicotine and nornicotine levels in 25 tobacco
genotypes.The highest nicotine content was recorded in FCR-15 (20.8 mg/g), and the lowest
nicotine content was observed in FCJ-11 (9.1 mg/g). Among 25 tobacco genotypes, nornicotine
content ranges from 7.74 mg/g (Bio Mut-55-1) to 0.0003 mg/g (LT Kanchan). Significant variation
was further observed in nicotine-to-nornicotine conversion rates.The Burley genotype Bio Mut
55- 1 exhibited the highest conversion rate (41.7%), followed by Banket A1 (8.453%). Importantly,
several genotypes showed negligible nornicotine accumulation. The genotypes with low
nornicotine accumulation provide valuable insight for breeding low-TSNA tobacco genotypes,
thereby improving the safety profile of tobacco products.
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