Isolation and detection of low molecular weight cationic peptide in milk of mastitic buffaloes

IRUNGBAM KARUNA DEVI; SANDEEP KUMAR GUPTA; S P S SANGHA; RAMNEEK RAMNEEK; C S AHUJA; RENU GUPTA

doi:10.56093/ijans.v82i11.25181

Authors

IRUNGBAM KARUNA DEVI Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
SANDEEP KUMAR GUPTA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
S P S SANGHA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
RAMNEEK RAMNEEK Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
C S AHUJA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
RENU GUPTA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India

https://doi.org/10.56093/ijans.v82i11.25181

Keywords:

AU-PAGE, Cationic peptides, Ion exchange chromatography, Mastitis, SDS-PAGE, Tricine SDS-PAGE

Abstract

Milk samples from mastitic and healthy buffaloes were subjected to centrifugation for preparation of acellular milk. It was subjected to ion exchange chromatography using carboxy methyl resin for purification. The purified protein and peptides obtained by the ion exchange chromatography were subjected to acid urea-polyacrylamide gel electrophoresis using lysozyme as a marker. The milk samples from mastitic buffaloes contained many cationic proteins and peptides. Some of these peptides exhibited migration similar to lysozyme. The SDS-PAGE revealed the presence of low molecular weight peptides in range of ~3–5KDa in mastitic milk, whereas these low molecular weight peptides were absent in milk from healthy animals. Tricine SDS PAGE was used for the resolution of peptides using high concentration of Acryl amide (49.5%) for resolving proteins less than 5KDa. It also revealed the presence of low molecular weight peptide of ~3–4.5KDa in mastitic milk and absence of such peptides from normal healthy buffalo’s milk. The present work has detected the presence of low molecular weight cationic peptides in milk of mastitic Buffaloes.

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Author Biographies

IRUNGBAM KARUNA DEVI, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
SANDEEP KUMAR GUPTA, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
S P S SANGHA, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
RAMNEEK RAMNEEK, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
C S AHUJA, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India
RENU GUPTA, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004 India

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