Correlation analysis between single nucleotide polymorphism of interleukin-1ß gene and interleukin-1 concentration in three chicken breeds


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Authors

  • LIU XIANGPING
  • WANG CUNBO
  • CHANG GUOBIN
  • WANG KEHUA
  • CHEN GUOHONG
  • DOU TAOCUN
  • QU LIANG
  • MA TENG
  • CHEN RONG

Keywords:

Chicken, Interleukin-1, Interleukin-1ß gene, SNP

Abstract

This paper aims to detect single nucleotide polymorphism (SNP) of interleukin-1ß (IL-1ß) gene of chicken and find
genetic marker of IL-1 concentration traits. SNPs of IL-1ß gene and IL-1 concentration in blood serum in Rugao (RG),
Wenchang (WC), and Anka Chicken (AK) were detected by SSCP-PCR and ELISA, respectively. The results showed
that IL-1 concentration in blood serum of AK was extremely significantly higher than that of RG and WC. Four mutations
were found in all 3 breeds, G476A, T482C, C1215T and T1507C. The genotype distribution of RG chicken at site nt476
was extremely significantly different from WC and AK, whereas there was no significant difference between WC and
AK. Significant correlations were found between C1215T and IL-1 concentration in 3 chickens breeds, showing that
IL-1 concentration of TT genotype chicken was significantly higher than that of CC. The results demonstrated that the
genotype distributions at SNP sites presented significant difference among three breeds, and the site nt1215 could be a genetic marker of IL-1 concentration traits in RG.

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Author Biographies

  • LIU XIANGPING
    58 Cangjie Road,Hanjiang District, Yangzhou City, Jiangsu Province, China 225125
  • WANG CUNBO
    College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225 125 China

  • CHANG GUOBIN
    College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225 125 China
  • WANG KEHUA
    58 Cangjie Road,Hanjiang District, Yangzhou City, Jiangsu Province, China 225125
  • CHEN GUOHONG
    College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225 125 China
  • DOU TAOCUN
    58 Cangjie Road,Hanjiang District, Yangzhou City, Jiangsu Province, China 225125
  • QU LIANG
    58 Cangjie Road,Hanjiang District, Yangzhou City, Jiangsu Province, China 225125
  • MA TENG
    College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
  • CHEN RONG
    College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

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Issue

Vol. 83 No. 4 (2013)

Section

Articles

License

The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

XIANGPING, L., CUNBO, W., GUOBIN, C., KEHUA, W., GUOHONG, C., TAOCUN, D., LIANG, Q., TENG, M., & RONG, C. (2013). Correlation analysis between single nucleotide polymorphism of interleukin-1ß gene and interleukin-1 concentration in three chicken breeds. The Indian Journal of Animal Sciences, 83(4). https://epubs.icar.org.in/index.php/IJAnS/article/view/28926