Phylogenetic analysis of CPV isolate of Manipur

RAJKUMARI SANJUKTA; MAHESH KUMAR; RAJKUMARI MANDAKINI; JOKEN BAM; I KARUNA; NAGAPPA K; S P SINGH

doi:10.56093/ijans.v84i10.44254

Authors

RAJKUMARI SANJUKTA ICAR RC NEH, Umiam, Meghalaya
MAHESH KUMAR Gobin Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India
RAJKUMARI MANDAKINI College of Veterinary Sciences and Animal Husbandary, CAU, Mizoram
JOKEN BAM NRC on Yak, Dirang, Arunachal Pradesh
I KARUNA IVRI, Izatnagar
NAGAPPA K Bombay Veterinary College, MAFSU, Parel, Mumbai
S P SINGH Gobin Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India

https://doi.org/10.56093/ijans.v84i10.44254

Keywords:

Analysis of Sequence, Canine parvovirus, Manipur, Molecular characterization

Abstract

This study was undertaken to determine the prevalent strain and epidemiological pattern of CPV isolates from Manipur. PCR was done with type-specific primers sets pCPV-2, pCPV-2ab and pCPV-2b. Out of 34 samples, 18 CPV isolates were obtained, all of which belong to CPV- 2b. This is the first report of strain characterization of CPV isolates from Manipur (Eastern India) so it was further confirmed by sequencing of the cloned PCR product of 427 bp size. GeneBank accession no. EU118267 was assigned to the sequence. Comparative analysis of the sequence has shown to have cent percent homology with CPV-Japan 97– 008, CPV Africa-3 and CPV-Polish isolates.

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