Effect of supplemental malic acid on methane mitigation in paddy straw based complete diet for sustainable animal production in indigenous dairy cattle


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Authors

  • A BHARATHIDHASAN Tamil Nadu Veterinary and Animal Sciences University, Vellore, Tamil Nadu 632 009 India

https://doi.org/10.56093/ijans.v92i11.100033

Keywords:

Dairy cattle, In vitro, In vivo methane reduction, Malic acid

Abstract

A study was conducted to evaluate the effect of supplemental malic acid on mitigation of methane emission for dairy cattle by in vitro and in vivo methods. The in vitro finding was validated by in vivo feeding trial in indigenous dairy cattle. Ten dairy cattle with uniform milk production were selected and divided into two groups with five animals each and they were fed with and without supplementation of malic acid at 0.39% in 60% paddy straw and 40% concentrate mixture based complete diet. The malic acid at 0.39% was the minimum level which resulted in highly significant reduction of methane by 15.95% and methane (ml) per 100 mg of truly digested substrate by 15.69%, respectively than control in in vitro study. The methane emission per animal per day and per kg dry matter intake (DMI) was significantly decreased by 3.26% and 3.11%, respectively in malic acid supplemented group than control. The methane emission per kg milk production was significantly reduced by 5.43% in malic acid supplemented group than control. The total volatile fatty acid (TVFA) and propionic acid were significantly increased by 2.69% and 11.71%, respectively in malic acid supplemented group than control. It was concluded that the supplementation of malic acid at 0.39% of paddy straw based complete diet significantly reduced the methane emission per animal per day and per kg milk production than control in indigenous dairy cattle.

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References

Asanuma N, M Iwamoto and T Hino. 1999. Effect of addition of fumarate on methane production by ruminal microorganisms in vitro. Journal of Dairy Science 82: 780–87. DOI: https://doi.org/10.3168/jds.S0022-0302(99)75296-3

Beauchemin K A and McGinn S M. 2006. Methane emissions from beef cattle: Effects of fumaric acid, essential oil and canola oil. Journal of Animal Science 84: 1489–96. DOI: https://doi.org/10.2527/2006.8461489x

Bharathidhasan A, Balamurugan R, Karuakaran R, Balakrishnan V, Pugazhenthi T R and Ezhilvalavan S. 2016. Influence of malic acid and fumaric acid on methane reduction in paddy straw based diet for ruminants. Proceedings of XVI Biennial Animal Nutrition Conference on Inovative Approaches for Animal Feeding and Nutritional Research held at NDRI, Karnal, India on 06-08, February 2016. Pp:86

Bharathidhasan A. 2020. In vitro evaluation of supplemental malic acid on methane mitigation and rumen fermentation characteristics for dairy cattle in forage based diet. Indian Veterinary Journal 97(8): 15–18.

Carro M D, Ranilla M J, Giraldez F J and Mantecon A R. 2006. Effects of malate on diet digestibility, microbial protein synthesis, plasma metabolites, and performance of growing lambs fed a high concentrate diet. Journal of Animal Science 84: 405–10. DOI: https://doi.org/10.2527/2006.842405x

Chaves A V, Thompson L C, Iwaasa A D, Scott S L, Olson M E, Benchaar C, Veira D M and McAllister T A. Effect of pasture type (alfalfa vs. grass) on methane and carbon dioxide production by yearling beef heifers. Canadian Journal of Animal Science 86: 409–18. DOI: https://doi.org/10.4141/A05-081

Chase L E. 1990. Analysis of fatty acids by packed column gas chromatography.G.C., Bulletin 856, Division of Rohand Has. Supelcom, pp: 1–12.

Foley P A, Kenny D A, Lovett D K, Callan J J, Boland T M and Mara F P O. 2009a. Effect of DL-malic acid supplementation on feed intake, methane emissions, and performance of lactating dairy cows at pasture. Journal of Dairy Science 92(7): 3258–64. DOI: https://doi.org/10.3168/jds.2008-1633

Foley P A, Kenny D A, Callan J J, Boland T M and Mara F P O. 2009b. Effects of di-malic acid supplementation on feed intake, methane emission and rumen fermentation in beef cattle. Journal of Animal Science 87: 1048–57. DOI: https://doi.org/10.2527/jas.2008-1026

Gall L S, Burroughs W, Gerlaugh P and Edgington B H. 1949. Special methods for rumen bacterial studies in the field. Journal of Animal Science 8: 433–40. DOI: https://doi.org/10.2527/jas1949.83433x

Gomez J A, Tejido M J and Carro M D. 2005. Influence of disodium malate on microbial growth and fermentation in rumen -simulation technique fermenters receiving medium and high concentrate diets. British Journal of Nutrition 93: 479–84. DOI: https://doi.org/10.1079/BJN20041367

IPCC. 2001. Inter-governmental Panel on Climate Change: The Scientific Basis. Contribution of working group I to the third assessment report of the intergovernmental panel on climate change. Cambridge, UK: Cambridge University Press.

Jalc D, Kisidayova S and Nerud F. 2002. Effects of plant oils and organic acid on rumen fermentation in vitro. Folia Microbiologica 47(2):171–77. DOI: https://doi.org/10.1007/BF02817677

Johnson K A and Johnson D E. 1995. Methane emissions from cattle. Journal of Animal Science 73: 2483–92. DOI: https://doi.org/10.2527/1995.7382483x

Kung L Jr., Huber J T, Krummrey J D, Allison L and Cook R M. 1982. Influence of adding malic acid to dairy cattle rations on milk production, rumen volatile acids, digestibility, and nitrogen utilization. Journal of Dairy Science 65: 1170–74. DOI: https://doi.org/10.3168/jds.S0022-0302(82)82328-X

Khampa S, Chumpawadee S and Wanapat M. 2009. Supplementation of malate level and cassava hay in high quality feed block on ruminal fermentation efficiency and digestibility of nutrients in lactating dairy cows. Pakistan Journal of Nutrition 8(4):441–46. DOI: https://doi.org/10.3923/pjn.2009.441.446

Li X Z, Choi S H, Jin G L, Yan C G, Long R J, Liang C Y and Song M K. 2009. Linolenic acid in association with malate or fumarate increased CLA production and reduced methane generation by rumen microbes. Asian–Australian Journal of Animal Science 6: 819–26. DOI: https://doi.org/10.5713/ajas.2009.80682

Lila Z A, Mohammed N, Tatsuoka N, Kanda S, Kurokawa Y and Itabashi H. 2004. Effect of cyclodextrin diallyl maleate on methane production, ruminal fermentation and microbes in vitro and in vivo. Animal Science Journal 75: 15–22. DOI: https://doi.org/10.1111/j.1740-0929.2004.00149.x

Lopez S, Valdes C, Newbold C J and Wallace R J.1999. Influence of sodium fumarate addition on rumen fermentation in vitro. British Journal of Nutrition 8: 59–64. DOI: https://doi.org/10.1017/S000711459900015X

Molano G T, Knight W and Clark H. 2008. Fumaric acid supplements have no effect on methane emissions per unit of feed intake in wether lambs. Australian Journal of Experimental Agriculture Science 48: 165–68. DOI: https://doi.org/10.1071/EA07280

Martin S A. 1998. Manipulation of rumen fermentation with organic acids: A review. Journal of Animal Science 76: 3123–32. DOI: https://doi.org/10.2527/1998.76123123x

Menke K H and Steingass H. 1988. Estimation of the energetic feed value obtained from chem gas production using rumen fluid. Animal Research and Development 28: 7–55.

Moir R J. 1951. The seasonal variation in the ruminal microorganism of grazing sheep. Australian Journal of Agricultural Research 27: 322. DOI: https://doi.org/10.1071/AR9510322

Moss A R, Jouany J P and Newbold C J. 2000. Methane production by ruminants: Its contribution to global warming. Annals de Zootechnie 49: 231–35. DOI: https://doi.org/10.1051/animres:2000119

Newbold C J, Ouda J O, Lopez S, Nelson N, Omed H, Wallace R J and Moss A R. 2002. Propionate precursors as possible alternative electron acceptors to methane in ruminal fermentation. (Eds) Takahashi J, Young B A, Soliva C R and Kreuzer M. The 1st International Conference on Green House Gases animal Agriculture GGAA2001. Elsvier Health Sciences. Tokachi Plaza, Japan, pp: 272–279.

Patra A K and Saxena J. 2010. A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry 71: 1198–1222. DOI: https://doi.org/10.1016/j.phytochem.2010.05.010

Russell J B. 1998. The importance of pH in the regulation of ruminal acetate to propionate ratio and methane production. Journal of Dairy Science 81: 3222–30. DOI: https://doi.org/10.3168/jds.S0022-0302(98)75886-2

Sitaula B K, Luo J and Bakkan L R. 1992. Rapid analysis of climate gases by wide bore capillary gas chromatography. Journal of Environmental Quality 21: 493–96. DOI: https://doi.org/10.2134/jeq1992.00472425002100030030x

Snedecor G W and Cochran W C. 1989. Statistical Methods, 8th edn. Iowa State University Press, Ames, Iowa.

Sniffen C J, Ballrd C S, Carter M P, Cotanch K W, Dann H M, Grant R J, Mandebvu P, Suekawa M and Martin S A. 2006. Effects of malic acid on microbial efficiency and metabolism in continuous culture of rumen contents and on performance of mid-lactation dairy cows. Animal Feed Science Technology 127: 13–31. DOI: https://doi.org/10.1016/j.anifeedsci.2005.07.006

Tejido M L, Ranilla M J, Garcia-Martinez R and Carro M D. 2005. In vitro microbial growth and rumen fermentation of different substrates as affected by the addition of disodium malate. Animal Science 81: 31–38. DOI: https://doi.org/10.1079/ASC42060031

Van Soest P J and Robertson. 1988. A Laboratory Manual for Animal Science, pp. 612. Ithaca NY: Cornell University Vargas J, Ungerfeld E, Muñoz C, DiLorenzo N. 2022. Feeding strategies to mitigate enteric methane emission from ruminants in grassland systems. Animals 12: 1132. DOI: https://doi.org/10.3390/ani12091132

Wallace R J, Wood T A, Rowe A, Price J, Yanez D R, Williams S P and Newbold C J. 2006. Encapsulated fumaric acid as a means of decreasing ruminal methane emissions. International Congress Series 1293: 148–51. DOI: https://doi.org/10.1016/j.ics.2006.02.018

Williams S R O, Moate P J, Hannah M C, Ribaux B E, Wales W J and Eckard R J. 2011. Background matters with the SF6 tracer method for estimating enteric methane emissions from dairy cows: a critical evaluation of the SF6 procedure. Animal Feed Science Technology 170: 265–76. DOI: https://doi.org/10.1016/j.anifeedsci.2011.08.013

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Submitted

2020-04-12

Published

2022-11-09

How to Cite

BHARATHIDHASAN, A. (2022). Effect of supplemental malic acid on methane mitigation in paddy straw based complete diet for sustainable animal production in indigenous dairy cattle. The Indian Journal of Animal Sciences, 92(11), 1314–1319. https://doi.org/10.56093/ijans.v92i11.100033

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