Expression profiling of host long non-coding RNAs under ORF virus infection

ARUNA PANDEY; SHIKHA SAXENA; RAJA ISHAQNABI KHAN; RAVI KUMAR GANDHAM; PRAMOD W RAMTEKE

doi:10.56093/ijans.v90i3.102319

Authors

ARUNA PANDEY ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
SHIKHA SAXENA ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
RAJA ISHAQNABI KHAN ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
RAVI KUMAR GANDHAM ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
PRAMOD W RAMTEKE ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v90i3.102319

Keywords:

Apoptosis, lncRNAs, ORFV, RNA-Seq, Sheep

Abstract

The present study aimed at gaining insights into the expression profile of long non-coding RNAs (lncRNAs) and coding genes of Orf virus (ORFV) infected oral mucosal tissues of sheep using RNA-Seq technology. Specifically, an expression profile and lncRNA-mRNA interaction network was inferred from a large-scale gene expression data set of sheep mucosal tissues on 0, 3, 7 and 15 days after ORFV infection. RNA-Seq profiles were obtained from the Gene Expression Omnibus (GEO) database. We found that 45, 64 and 45 lncRNAs and 1796, 2182 and 1550 coding genes were differentially expressed at early (T3), intermediate (T7) and late (T15) stages of ORFV infection in sheep mucosal tissues, respectively. Functional analysis revealed that differentially expressed long non-coding RNAs (DElncRNAs) regulate immune processes by regulating the expression level of differentially expressed coding genes (DEGs) under ORFV infection.

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