Methicillin resistant Staphylococcus aureus (MRSA) from community associated settings
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Keywords:
Antibiotic resistance (AR), Antibiotic resistance genes, Community, Epsilometer test, MIC values, Multidrug resistance (MDR), Staphylococcus aureusAbstract
Antibiotic resistance is a cause of concern worldwide. Community settings are important reservoir of drug resistant microorganisms like Staphylococcus aureus. The present study was to determine the prevalence, phenotypic and genotypic antibiotic resistance pattern of S. aureus isolated from different community settings of university campus. A total of 300 swab samples were collected for isolation of S. aureus from different community settings at university campus of Guru Angad Dev Veterinary and Animal Sciences University and Punjab Agriculture University, Ludhiana, India. Confirmed S. aureus isolates were further subjected to antibiotic sensitivity by Epsilometer test (E-test) and detection of antibiotic resistance genes. The prevalence of S. aureus in the community samples was 12% (36/300). Methicillin Resistant S. aureus (MRSA) contamination among community was 3.33% (10/300). Among S. aureus isolates from community samples 63.8% (23/36) and all the MRSA isolates were multidrug resistant (MDR). Five out of 10 MRSA carried SCCmec type IVa, and 4 were pvl positive gene, therefore, designated as community associated MRSA (CA-MRSA). Phenotypic resistance to antibiotics ciprofloxacin, chloramphenicol, ceftriaxone clindamycin and trimethoprim-sulfamethoxazole was 69.4% (MIC ≥32 μg/ml), 63.9% (MIC 32 μg/ ml), 16.7% (MIC 16–64 μg/ml), 16.7% (MIC 256 μg/ml) and 8.3% (MIC 12–64 μg/ml), respectively. Resistance genes blaZ, mecA, tetK, tetM, ermB and aacA-aphD were present. Presence of MRSA and MDR variant in community settings is a public health concern, as cell phone, offices telephone, computer keyboard and tap faucet are commonly shared or touched by people. Therefore, have potential to disseminate widely, not only in the community settings but also in hospitals environment, complicating treatment.
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References
Al-Abdalall A H. 2010. Isolation and identification of microbes associated with mobile phones in Dammam in eastern Saudi Arabia. Journal of Family and Community Medicine 17(1): 11–14. DOI: https://doi.org/10.4103/1319-1683.68783
Aso J O, Kolawole D O, Aso O O and Ajisebutu S O. 2011. Antibiotic sensitivity pattern of Staphylococcus aureus from fomites in the Obafemi Awolowo University Teaching Hospital Complex (OAUTHC) Nigeria. International Journal of Medical Science 3(2): 32–36.
Dan Dan Sun, Xiao Xue Ma, Jian Hu, Yuan Tian, Long Pang, Hong Shang and Long Zhu Cui. 2013. Epidemiological and molecular characterization of community and hospital acquired Staphylococcus aureus strains prevailing in Shenyang, Northeastern China. Brazilian Journal of Infectious Diseases 17(6): 682–90. DOI: https://doi.org/10.1016/j.bjid.2013.02.007
Duran N, Ozer B, Duran G G, Onlen Y and Demir C. 2010. Antibiotic resistance genes and susceptibility patterns in staphylococci. Indian Journal of Medical Research 135: 389– 96.
Frye J G and Fedorka-Cray P. 2007. Prevalence, distribution and characterisation of ceftiofur resistance in Salmonella enterica isolated from animals in the USA from 1999 to 2003. DOI: https://doi.org/10.1016/j.ijantimicag.2007.03.013
International Journal of Antimicrobial Agents 30(2): 134–42. Irlinger F. 2008. Safety assessment of dairy microorganisms: Coagulase-negative staphylococci. International Journal of Food Microbiology 126: 302–10. DOI: https://doi.org/10.1016/j.ijfoodmicro.2007.08.016
Kaseem I I, Von Sigler and Esseili M A. 2007. Public computer surfaces are reservoirs for methicillin-resistant staphylococci. ISME Journal 1: 265–68. DOI: https://doi.org/10.1038/ismej.2007.36
Moellering R C. 2006. The growing menace of communityacquired methicillin resistant Staphylococcus aureus. Annals of Internal Medicine 144: 368–70. DOI: https://doi.org/10.7326/0003-4819-144-5-200603070-00014
Morgan M. 2008. Methicillin-resistant Staphylococcus aureus and animals: Zoonosis or humanosis? The Journal of Antimicrobial Chemotherapy 62: 1181–87. DOI: https://doi.org/10.1093/jac/dkn405
Oie S, Hosokawa I and Kamiya A. 2002. Contamination of room door handles by methicillin-sensitive/methicillin-resistant Staphylococcus aureus. Journal of Hospital Infection 51: 140– 43. DOI: https://doi.org/10.1053/jhin.2002.1221
Roberts M C, Soge O O and No D. 2013. Comparison of multidrug resistant environmental methicillin-resistant Staphylococcus aureus isolated from recreational beaches and high touch surfaces in built environments. Frontiers in Microbiology 4: 74. DOI: https://doi.org/10.3389/fmicb.2013.00074
Roberts M C, Soge O O, No D, Helgeson S E and Meschke J S. 2011. Characterization of methicillin-resistant Staphylococcus aureus isolated from public surfaces on a university campus, student homes and local community. Journal of Applied Microbiology 110: 1531–37. DOI: https://doi.org/10.1111/j.1365-2672.2011.05017.x
Shahaby A F, Awad N S, EI-Tarras A E and Bahobial A S. 2012. Mobile phone as potential reservoirs of bacterial pathogens. African Journal of Biotechnology 11: 15896–904. DOI: https://doi.org/10.5897/AJB12.1836
Shopsin B, Mathema B, Martinez J, Ha E, Campo M L, Fierman A, Krasinski K, Kornblum J, Alcabes P, Waddington M, Riehman M and Krieswirth B N. 2000. Prevalence of methicillin-resistance and methicillin-susceptible Staphylococcus aureus in the community. The Journal of Infectious Diseases 182: 359–62. DOI: https://doi.org/10.1086/315695
Strommenger B, Kettlitz C, Werner G and Witte W. 2003. Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. Journal of Clinical Microbiology 41: 4089–94. DOI: https://doi.org/10.1128/JCM.41.9.4089-4094.2003
Tambekar D H, Dhanorkar D V, Gulhane S R and Dudhane M N. 2007. Prevalence and antimicrobial susceptibility pattern of Methicillin Resistant Staphylococcus aureus from healthcare and community associated sources. African Journal of Infectious Diseases 1: 52–56. DOI: https://doi.org/10.4314/ajid.v1i1.42086
Torimiro N and Torimiro S E A. 2012. Antibiotic resistance profile in community-associated Staphylococcus aureus strains isolated from a Nigerian peri-urban community. African Journal of Biotechnology 11(94): 16071–76. DOI: https://doi.org/10.5897/AJB12.2397
Ugwu M C, Ikegbunam M N, Nduka S O, Attama A A, Ibezim E C and Esimone C O. 2013. Molecular characterization and efficacy of antibiotic combination on multiple antibioticresistant Staphylococcus aureus isolated from nostrils of healthy human volunteers. Journal of Pharmacological Sciences and Research 5(1): 26–32.
Wang X, Meng J, Zhou T, Zhang Y, Yang B, Xi M, Sheng J, Zhi S and Xia X. 2012. Antimicrobial Susceptibility Testing and Genotypic Characterization of Staphylococcus aureus from Food and Food Animals. Foodborne Pathogens and Disease 9(2): 95–101. DOI: https://doi.org/10.1089/fpd.2011.0987
Zehra A, Singh R, Kaur S and Gill J P S. 2017. Molecular characterization of antibiotic-resistant Staphylococcus aureus from livestock (bovine and swine). Veterinary World 10(6): 598–604. DOI: https://doi.org/10.14202/vetworld.2017.598-604
Zhang K, McClure J A, Elsayed S, Louie T and Conly J M. 2005. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. Journal of Clinical Microbiology 43: 5026–33. DOI: https://doi.org/10.1128/JCM.43.10.5026-5033.2005
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