Comparative efficacy of three extenders on quality of boar semen during preservation at 15°C


158 / 120

Authors

  • TRACY FREDHA M SANGMA Assam Agricultural University, Guwahati, Assam 781 022 India
  • KUTUBUDDIN AHMED Assam Agricultural University, Guwahati, Assam 781 022 India
  • MITALI DUTTA CHOUDHURY Assam Agricultural University, Guwahati, Assam 781 022 India
  • GALIB UZ ZAMAN Assam Agricultural University, Guwahati, Assam 781 022 India
  • NEKIBUDDIN AHMED Assam Agricultural University, Guwahati, Assam 781 022 India
  • ARUNODAY DAS Assam Agricultural University, Guwahati, Assam 781 022 India

https://doi.org/10.56093/ijans.v90i3.102439

Keywords:

Boar, Extender, Fertility, GEPS, Modena, Preservation, Semen quality, TRIXcell

Abstract

The present study was designed to compare the efficacy of 3 different extenders for preservation of boar semen at 15°C. Ejaculates (32) were evaluated and extended (1:4) in TRIXcell+, Modena and GEPS extenders and kept for 4 h of holding at 22°C. The samples were preserved in a BOD incubator at 15°C up to 168 h and evaluated for sperm motility, intact acrosome and HOST-reacted sperm at 0, 72, 96, 120, 144 and 168 h of preservation. The mean percentage of sperm motility, intact acrosome and HOST-reacted sperm was significantly higher in TRIXcell+ extender than Modena and GEPS at different hours of preservation. Out of the 3 extenders studied, TRIXcell+ was found to be the best followed by Modena and GEPS extenders respectively based on sperm motility, intact acrosome and HOST.

Downloads

Download data is not yet available.

References

Althouse G C, Wilson M E, Kuster C and Parsley M. 1998. Characterization of lower temperature storage limitations of fresh extended porcine semen. Theriogenology 50: 535–43. DOI: https://doi.org/10.1016/S0093-691X(98)00159-9

Bania B K. 2017. ‘Technology validation of semen preservation, synchronization of estrus and artificial insemination in pig’. MVSc Thesis, Assam Agricultural University, Khanapara, Guwahati.

Bresciani C, Morini G, Bettini R, Bigliardi E, Ianni F D, Cabassi C S, Sabbioni A and Parmigiani E. 2013. Reproductive efficiency of new modified boar semen extender for liquid storage. Livestock Science 157: 384–88. DOI: https://doi.org/10.1016/j.livsci.2013.07.005

deLamirande E and Gagnon C. 1995. Impact of reactive oxygen species on spermatozoa: A balancing act between beneficial and detrimental effects. Human Reproduction 10: 15–21. DOI: https://doi.org/10.1093/humrep/10.suppl_1.15

Dimitrov S, Atanasov V, Dichlyanova E and Petrova R. 2009. Comparison of three commercial diluents for short-term storage of boar semen. Trakia Journal of Sciences 7: 58–62.

Frydrychova S, Cerovsky J, Lustykova A and Rozkot M. 2010. Effects of long-term liquid commercial semen extender and storage time on the membrane quality of boar semen. Czech Journal of Animal Science 55: 160–66. DOI: https://doi.org/10.17221/62/2009-CJAS

Gadea J. 2003. Review: Semen extenders used in artificial insemination of swine. Spanish Journal of Agricultural Research 1: 17–27. DOI: https://doi.org/10.5424/sjar/2003012-17

Gilmore J A, Liu J, Peter A T and Crister J K. 1998. Determination of plasma membrane characteristics of boar spermatozoa and their relevance to cryopreservation. Biological Reproduction 58: 28–36. DOI: https://doi.org/10.1095/biolreprod58.1.28

Johnson L A, Gerrits R J and Young E P. 1969. The fatty acid composition of porcine spermatozoa phospholipids. Biology of Reproduction 1: 330–34. DOI: https://doi.org/10.1095/biolreprod1.4.330

Johnson L A, Weitze K F, Fiser P and Maxwell W M C. 2000. Storage of boar semen. Animal Reproduction Science 62: 143– 72. DOI: https://doi.org/10.1016/S0378-4320(00)00157-3

Jones R and Mann T. 1977. Damage to ram spermatozoa by peroxidation and apoptosis like changes in spermatozoa. Animal Reproduction Sciences 110: 162–71.

Kaeoket K, Srisowanna T, Wichaidit U, Chanapiwat P and Manee- In S. 2010. Comparative study on six different long term commercial extenders for fresh boar semen. Thai Journal of Veterinary Medicine 40: 257–63.

Kumaresan A, Kadirvel G, Bujarbaruah K M, Bardoloi R K, Das A, Kumar S and Naskar S. 2009. Preservation of boar semen at 18ºC induces lipid peroxidation and apoptosis like changes in spermatozoa. Animal Reproduction Science 110: 162–71. DOI: https://doi.org/10.1016/j.anireprosci.2008.01.006

Lalrintluanga K, Deka B C, Nath K C, Hmar L, Bhuyan D and Biswas R K. 2016. Effect of different extenders on the quality of boar semen during preservation at 18°C. International Journal of Multidisciplinary Approach and Studies 3: 224– 32.

Saikia T, Ahmed K, Tamuli M K and Ahmed N. 2016. Effect of different extenders on quality of boar semen during preservation at 15°C and 5°C. Theriogenology Insight 6: 73– 77. DOI: https://doi.org/10.5958/2277-3371.2016.00010.3

Saleh R A and Agarwal A. 2002. Oxidative stress and male infertility: From research bench to clinical practice. Journal of Andrology 23: 737–52.

Sa S J, Kim I C, Choi S H, Hong J K, Kim D W, Cho K H, Kim Y H, Chung K H and Park J C. 2013. Effects of storage in different commercial semen extenders on sperm motility, viability and membrane integrity of Korean Native boar spermatozoa. Journal of Embryology Transfer 28: 349–53. DOI: https://doi.org/10.12750/JET.2013.28.4.349

Revell S G and Mrode R A. 1994. An osmotic resistance test for bovine semen. Animal Reproduction Science 36: 77–86. DOI: https://doi.org/10.1016/0378-4320(94)90055-8

vanden Berg B M, Reesink J and Reesink W. 2014. TRIXcell+, a new long term boar semen extender containing whey protein with higher preservation capacity and litter size. Open Veterinary Journal 4: 20–25. DOI: https://doi.org/10.5455/OVJ.2014.v4.i1.p20

Waberski D, Petrunkina A M and Topfer-Petersen E. 2008. Can external quality control improve pig AI efficacy? Theriogenology 70: 1346–51. DOI: https://doi.org/10.1016/j.theriogenology.2008.06.006

Watson P F. 1975. Use of Giemsa stain to detect change in acrosomes of frozen ram spermatozoa. Veterinary Record 97: 12–15. DOI: https://doi.org/10.1136/vr.97.1.12

Weitze K F. 1991. Long-term storage of extender boar semen. Reproduction in Domestic Animals 26: 231–53.

Zou C and Yang Z. 2000. Evaluation on sperm quality of freshly ejaculated boar semen during in vitro storage under different temperatures. Theriogenology 53: 1477–99. DOI: https://doi.org/10.1016/S0093-691X(00)00290-9

Downloads

Submitted

2020-07-15

Published

2020-07-17

Issue

Section

Articles

How to Cite

M SANGMA, T. F., AHMED, K., CHOUDHURY, M. D., ZAMAN, G. U., AHMED, N., & DAS, A. (2020). Comparative efficacy of three extenders on quality of boar semen during preservation at 15°C. The Indian Journal of Animal Sciences, 90(3), 375-378. https://doi.org/10.56093/ijans.v90i3.102439
Citation