Exogenous catalase improves the goat seminal attributes at refrigeration temperature

PALLAVI SINGH; R RANJAN; S D KHARCHE; M K SINGH

doi:10.56093/ijans.v90i3.102441

Authors

PALLAVI SINGH ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh 281 122 India
R RANJAN ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh 281 122 India
S D KHARCHE ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh 281 122 India
M K SINGH ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh 281 122 India

https://doi.org/10.56093/ijans.v90i3.102441

Keywords:

Buck, Catalase, Refrigeration temperature, Semen analysis

Abstract

The objective of the present study was to evaluate the potential benefit of catalase (CAT) on sperm functional parameters (motility, viability, membrane integrity and acrosomal integrity) during liquid storage of buck spermatozoa at 0 h, 24 h, 48 h, 72 h and 96 h. Semen samples from bucks were pooled to avoid individual effect and diluted with tris-egg yolk-fructose extender with catalase (0 U/ mL (control), 200 U/mL, 400 U/ mL, 600 U/ mL and 800 U/ mL) at a final concentration of 400 million/mL. No significant difference was observed between treatments at 24 h with respect to motility, viability and plasma membrane integrity. However, acrosome integrity of control was significantly higher as compared to other concentrations. Motility, viability, plasma membrane and acrosome integrity were observed significantly highest for CAT @ 600 U/mL in comparison to other concentrations at 48 h, 72 h and 96 h of liquid storage at refrigeration temperature. The seminal parameters declined with the progression of storage time and remained over 50% even after 72 h at refrigeration temperature in CAT @ 600 U/mL. It can be concluded that the addition of CAT @ 600 U/mL in semen diluent along with 20% egg yolk reduced the detrimental effects of cooling on motility, viability, plasma membrane and acrosome integrity.

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References

Abu N, Md Rahman A, Abdullah R B and Khadijah W E W. 2008. A review of reproductive biotechnologies and their application in goats. Biotechnology 7(2): 371–84. DOI: https://doi.org/10.3923/biotech.2008.371.384

Aisen E G, Medina V H and Venturino A. 2002. Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology 57(7): 1801–08. DOI: https://doi.org/10.1016/S0093-691X(02)00653-2

Aitken R J. 2017. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Molecular Reproduction and Development 84: 1039–52. DOI: https://doi.org/10.1002/mrd.22871

Akhter S, Ansari M S, Rakha B A, Ullah N, Andrabi S M H and Khalid M. 2011. In vitro evaluation of liquid-stored Buffalo semen at 5°C diluted in soya lecithin-based extender (Bioxcell®), Tris-citric-egg yolk, skim milk and egg yolk citrate extenders. Reproduction in Domestic Animals 46(1): 45–49. DOI: https://doi.org/10.1111/j.1439-0531.2009.01561.x

Allai L, Benmoula A, Maia M S, Nasser B and Amiri B E. 2018. Supplementation of ram semen extender to improve seminal quality and fertility rate. Animal Reproduction Science 192: 6–17. DOI: https://doi.org/10.1016/j.anireprosci.2018.03.019

Anzar M, Kroetsch T and Boswall L. 2011. Cryopreservation of bull semen shipped overnight and its effect on post-thaw sperm motility, plasma membrane integrity, mitochondrial membrane and normal acrosomes. Animal Reproduction Science 126: 23– 31. DOI: https://doi.org/10.1016/j.anireprosci.2011.04.018

Ball B A, Medina V, Gravance C G and Baumber J. 2001. Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa at 5°C. Theriogenology 56(4): 577–89. DOI: https://doi.org/10.1016/S0093-691X(01)00590-8

Baumber J, Vo A, Sabeur K and Ball B A. 2002. Generation of reactive oxygen species by equine neutrophils and their effect on motility of equine spermatozoa. Theriogenology 57: 1025– 33. DOI: https://doi.org/10.1016/S0093-691X(01)00710-5

Brogan P T, Beitsma M, Henning H, Gadella B M and Stout T A. 2015. Liquid storage of equine semen: Assessing the effect of d-penicillamine on longevity of ejaculated and epididymal stallion sperm. Animal Reproduction Science 159: 155–62. DOI: https://doi.org/10.1016/j.anireprosci.2015.06.011

Camara D R, Pinto L C, Pinto M M C M, Kastelic J P, Nunes J F, Barbosa J M P and Guerra M M P. 2016. Influence of catalase and pre-freezing equilibration on post-thaw semen quality and conception rate in ewes laparoscopically inseminated. Animal Reproduction 13(1): 21–27. DOI: https://doi.org/10.4322/1984-3143-AR729

Falchi L, Galleri G, Zedda M T, Pau S, Bogliolo L, Ariu F and Ledda S. 2018. Liquid storage of ram semen for 96 h: Effects on kinematic parameters, membranes and DNA integrity, and ROS production. Livestock Science 207: 1–6. DOI: https://doi.org/10.1016/j.livsci.2017.11.001

Gaczarzewicz D, Udala J, Piasecka M, Blaszczyk B and Stankiewicz T. 2015. Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability. Turkish Journal of Biology 39: 582–94. DOI: https://doi.org/10.3906/biy-1412-76

Gangwar C, Kharche S D, Ranjan R, Kumar Satish, Goel A K and Jindal S K. 2015. Effect of vitamin C supplementation on freezability of Barbari buck semen. Small Ruminant Research 129: 104–07. DOI: https://doi.org/10.1016/j.smallrumres.2015.06.002

Gangwar C, Ranjan R, Kumar S, Kharche S D, Goel A K, Ramachandran N and Jindal S K. 2014. Use of chelating agent for optimum post thaw quality of buck semen. Indian Journal of Animal Sciences 84(8): 839–41.

Gungor S, Ata A and Inac M E. 2018. Effects of trehalose and catalase on the viability and kinetic parameters of cryopreserved ram semen. Acta Scientiae Veterinariae 46: 1–7. DOI: https://doi.org/10.22456/1679-9216.83865

Hammerstedt R H, Graham J K and Nolan J P. 1990. Cryopreservation of mammalian sperm: what we ask them to survive. Journal of Andrology 11: 73–88.

Hancock J L. 1951. A staining technique for the study of temperature shock in semen. Nature (London) 167: 323. DOI: https://doi.org/10.1038/167323b0

Kadirvel G, Kalita M K, Dewry R K, Kumar A, Mahanta N, Singh L A, Baishya S K and Ngachan S V. 2019. Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India. Indian Journal of Animal Research 53(1): 41–44. DOI: https://doi.org/10.18805/ijar.B-3464

Li J, Li Q, Wei G, Zhang J and Li Y. 2018. Effects of negative pressure on boar semen quality during storage at 17°C. Indian Journal of Animal Research 52(8): 1146–50. DOI: https://doi.org/10.18805/ijar.B-791

Maneesh M and Jayalekshmi H. 2006. Role of reactive oxygen species and antioxidants on pathophysiology of male reproduction. Indian Journal of Clinical Biochemistry 21: 80– 89. DOI: https://doi.org/10.1007/BF02912918

Perumal P, Chamuah J K and Rajkhowa C. 2013a. Effect of catalase on the liquid storage of mithun (Bos frontalis) semen. Asian Pacific Journal of Reproduction 2(3): 209–14. DOI: https://doi.org/10.1016/S2305-0500(13)60148-7

Perumal P, Vupru K and Khate K. 2013b. Effect of addition of melatonin on the liquid storage (5°C) of mithun (Bos frontalis) semen. International Journal of Zoology 2013: 1–10. DOI: https://doi.org/10.1155/2013/642632

Pietta P G. 2000. Flavonoids as antioxidants. Journal of Natural Products 63: 1035–42. DOI: https://doi.org/10.1021/np9904509

Ranjan R, Ramachandran N, Jindal S K and Sinha N K. 2009a. Hypo osmotic swelling test in frozen thawed goat spermatozoa. Indian Journal of Animal Sciences 79(10): 1022–23.

Ranjan R, Ramachandran N, Jindal S K and Sinha N K. 2009b. Effect of egg yolk levels on keeping quality of Marwari buck semen at refrigeration temperature. Indian Journal of Animal Sciences 79(7): 662–64.

Ranjan R, Goel AK, Ramachandran N, Kharche S D, Gangwar C and Jindal S K. 2014. Comparison between normal and dual staining technique for evaluating acrosome status and viability in frozen thawed buck spermatozoa. Indian Journal of Small Ruminants 20(2): 50–53.

Ranjan R, Priyadharsini R, Goel A K, Singh B, Kumar S, Kharche S D and Jindal S K. 2017. Effect of membrane stabilizer on the freezability of buck semen. Indian Journal of Animal Sciences 87(4): 435–36.

Ranjan R, Goel A K, Ramachandran N, Kharche S D and Jindal S K. 2015. Effect of egg yolk levels and equilibration periods on freezability of Jamunapari buck semen. Indian Journal of Small Ruminants 21(1): 32–36. DOI: https://doi.org/10.5958/0973-9718.2015.00027.6

Revell S G and Mrode R A. 1994. An osmotic resistance test for bovine semen. Animal Reproduction Science 36: 77–86. DOI: https://doi.org/10.1016/0378-4320(94)90055-8

Sanocka D and Kurpisz M. 2004. Reactive oxygen species and sperm cells. Reproductive Biology and Endocrinology 2: 1–7. DOI: https://doi.org/10.1186/1477-7827-2-12

Saraswat S, Priyadarshini R, Jindal S K, Yadav S, Ramachandran N, Kharche S D and Goel A K. 2012. Effect of antioxidants supplementation at refrigeration temperature on sperm motion characteristics and membrane integrity of Sirohi buck semen. Journal of Physiology and Pharmacology Advances 2(1): 77– 86.

Saraswat S, Jindal S K, Kharche S D, Rout P K, Ranjan R and Priyadharsini R. 2014. Role of antioxidant additives in the protection of DNA integrity of buck spermatozoa with RAPD assay. Indian Journal of Animal Sciences 84(3): 295–97.

Sikka S C. 1996. Oxidative stress and role of antioxidants in normal and abnormal sperm function. Frontiers in Bioscience 1: 78–86. DOI: https://doi.org/10.2741/A146

Tran L V, Malla B A, Kumar S and Tyagi A K. 2017. Polyunsaturated fatty acids in male ruminant reproduction-A review. Asian Australasian Journal of Animal Sciences 30(5): 622–37. DOI: https://doi.org/10.5713/ajas.15.1034

Urata K, Narahara H, Tanaka Y, Egashira T, Takayama F and Miyakawa I. 2001. Effect of endotoxin-induced reactive oxygen species on sperm motility. Fertility Sterility 76(1): 163–66. DOI: https://doi.org/10.1016/S0015-0282(01)01850-7

Watson P F. 1975. Use of Giemsa stain to detect changes in acrosome of frozen ram spermatozoa. Veterinary Records 97: 12–15. DOI: https://doi.org/10.1136/vr.97.1.12