Adaptability of a field isolate of Classical swine fever virus to PK-15 cells

R AKSHAYA; A RAMESH; K LOGESH; R SANGEETHA; K VIJAYARANI; K KUMANAN; P TENSINGH GNANARAJ; G DHINAKAR RAJ

doi:10.56093/ijans.v90i3.102515

Authors

R AKSHAYA Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
A RAMESH Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
K LOGESH Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
R SANGEETHA Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
K VIJAYARANI Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
K KUMANAN Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
P TENSINGH GNANARAJ Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
G DHINAKAR RAJ Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

https://doi.org/10.56093/ijans.v90i3.102515

Keywords:

Adaptation, Classical swine fever virus, FAVN, PK-15 cells, RT-PCR

Abstract

Classical swine fever (CSF) is an economically important pig disease affecting rural pig farming and vaccines are not freely available for control. In the present study an Indian isolate of CSF virus was passaged 75 times in PK-15 cells. At different passages, presence of virus was confirmed by Fluorescent Antibody Virus Neutralization (FAVN) test and Reverse Transcription-Polymerase Chain Reaction (RTPCR) for NS5B, E2 and 5-UTR genes. TCID50 titers were found to range between 4.00 and 8.97 at 10th and 75th passages respectively. Back passage and pathogenicity studies in susceptible pigs, the natural host, indicated that the virus was found to be attenuated following PK-15 passages and did not produce any clinical signs after 45th and 75th passages.

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