Fetal Leydig cells in buffalo testis: Light and electron microscopic study

INDERPREET SINGH; NEELAM BANSAL; DEVENDRA PATHAK; VARINDER UPPAL; ANURADHA GUPTA

doi:10.56093/ijans.v90i4.104193

Authors

INDERPREET SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
NEELAM BANSAL Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
DEVENDRA PATHAK Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
VARINDER UPPAL Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
ANURADHA GUPTA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India

https://doi.org/10.56093/ijans.v90i4.104193

Keywords:

Buffalo fetus, Electron microscopy, Histology, Leydig cells

Abstract

The present study was conducted on testis of 20 buffalo fetuses ranging from 2.5 cm to 20 cm curved crown rump length (CVRL). The tissues were processed for light and transmission electron microscopic study. The intertubular tissue was filled with primitive mesenchymal cells, developing Leydig cells and blood vessels. At 7.5 cm CVRL, some of the interstitial mesenchymal cells started developing into foetal Leydig cells and were androgen positive. These cells were characterized by their polygonal shape, eosinophilic cytoplasm and darkly stained spherical nucleus with prominent nucleoli at 9.5 cm CVRL. At 15.5 cm CVRL, the fetal Leydig cells were present in groups around the interstitial blood vessels, which increased in number and size at 20 cm CVRL and decreased afterwards. By TEM, three types of Leydig cells were observed at 14.5 cm CVRL as stem Leydig cells (Leydig cell A), progenitor Leydig cells (Leydig cell B) and immature Leydig cells (Leydig cell C). Leydig cell A had elongated nucleus with a very thin rim of cytoplasm. Leydig cell B had oval nucleus with comparatively larger amount of cytoplasm. Leydig cell C had adult Leydig cell like features and had round to oval nucleus with lager amount of cytoplasm. The cytoplasm of these cells contained mitochondria with elongated cristae, Golgi apparatus and very few lipid inclusions. The number and size of Leydig cells increased during early prenatal life.

Downloads

Download data is not yet available.

References

Abd-Elmaksoud A. 2005. ‘Morphological, glycohistochemical and immunohisto chemical studies on the embryonic and adult bovine testis’. PhD Thesis, Munich, Germany.

Baishya G and Vyas K N. 1990. Studies on foetal testicular development in Surti buffalo (Bubalus bubalis). Indian Veterinary Journal 68: 556–60.

Bansal N, Pathak D, Uppal V, Gupta A and Singh I. 2015. Androgen receptor in buffalo testis during prenatal life: An Immuno-histochemical study. Indian Journal of Veterinary Anatomy 27(2): 6–8.

Chen H, Stanley E, Jin S and Zirkin B R. 2010. Stem Leydig cells: From fetal to aged animals. Birth Defects Research Part C: Embryo Today: Reviews 90: 272–83. DOI: https://doi.org/10.1002/bdrc.20192

Codesal J, Regadera J, Nistal M, Regadera-sejas J and Paniagua R.1990. Involution of human fetal Leydig cells. An immunohistochemical, ultrastructural and quantitative study. Journal of Anatomy 172: 103–14

Edward M J. 1965. Observations on the anatomy of reproductive organs of cows with special reference to those features sought during examination per rectum. New Zealand Veterinary Journal 13(2): 25–37. DOI: https://doi.org/10.1080/00480169.1965.33588

Farooqui M M, Chandrapal A and Prakash A. 2012. Histological and histochemcial studies on the prenatal development of testis in goat (Capra hircus). International Journal of Morphology 30: 1408–21. DOI: https://doi.org/10.4067/S0717-95022012000400024

Huhtaniemi I and Pelliniemi L J. 1992. Fetal Leydig cells: Cellular origin, morphology, life span and special functional features. Proceedings of the Society for Experimental Biology and Medicine 201: 125–40. DOI: https://doi.org/10.3181/00379727-201-43493

Hullinger R L and Wensing C J. 1985. Testicular organogenesis in the fetal calf: interstitial endocrine (Leydig) cell development. Acta Anatomica 121(2): 99–109. DOI: https://doi.org/10.1159/000145951

Kaur M, Bansal N and Uppal V. 2011. Histogenesis of testicular parenchyma during prenatal life in buffalo. Indian Journal of Morphology 29: 1109–14. DOI: https://doi.org/10.4067/S0717-95022011000400006

Kaur M. 2006. ‘Studies on histogenesis and organogenesis of the testis in buffalo (Bubalus bubalis).’M.V.Sc. Thesis, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.

Kerr J B and Knell C M. 1988. The fate of fetal Leydig cells during the development of the fetal and postnatal rat testis. Development 103(3): 535–44. DOI: https://doi.org/10.1242/dev.103.3.535

Kuopio T, Tapanainen J, Pelliniemi L J and Huhtaniemi I. 1989. Developmental stages of fetal-type Leydig cells in prepubertal rats. Development 107: 213–20. DOI: https://doi.org/10.1242/dev.107.2.213

Luna L G. 1968. Manual of histological staining methods of the Armed Forces Institute of Pathology. 3rd ed. New York, Blakistan Division, Mc-Graw Hill Book Company.

Orth J M. 1993. Cell biology of testicular development in the fetus and neonate. Cell and Molecular Biology of the Testis. (Eds) Desjardins C and Ewing L L. Oxford University Press, Oxford.

Pachpande A M, Raut S S, Patil A D, Dhande P L, Gaikwad S A and Yadav S B. 2006.Histological and Histochemical Study of Testis and Epididymis during Prenatal Development in Sheep. Proc XX Convention IA VA, pp. 65, Jabalpur, India.

Padmini M P and Rao B N. 2012. Prenatal histogenesis of human fetal testis. International Journal of Basic and Applied Medical Sciences 2: 112–16.

Rebourcet D, O’Shaughnessy P J, Pitetti Jean-Luc, Monteiro A, O’Hara L, Milne L, Tsai Y T, Cruickshanks L, Riethmacher D, Guillou F, Mitchell R T, van ‘t Hof R, Freeman T C, Nef Serge and Smith L B. 2014. Sertoli cells control peritubular myoid cell fate and support adult Leydig cell development in the prepubertal testis. Development 141: 2139–49. DOI: https://doi.org/10.1242/dev.107029

Russe I. 1991. Lehrbuch der EmbioIogie der Haustiere. (Eds) Russe I and Sinowatz F. Paul Parey Verlag, Berlin, und Hamburg.

Singh Y, Sharma D N and Dhingra L D. 1979. Morphogenesis of the testis in goat. Indian Journal of Animal Sciences 49: 925– 31.

Sinowatz F, Amselgruber W and Russe I. 1987. Prä-und Postnatal Differenzierung der Leydig Zellenbeim Rind. Acta Europaea Fertilitatis 3: 191–96.

Soliman M K. 1975. Studies on the physiological chemistry of the allantoic and amniotic fluids of buffaloes at various periods of pregnancy. Indian Veterinary Journal 52: 111–17.

Wrobel K H. 1990. The postnatal development of the bovine Leydig cell population. Reproduction of Domestic Animals 25(2): 51–60. DOI: https://doi.org/10.1111/j.1439-0531.1990.tb00681.x