Comparative efficacy of different concentrations of egg yolk for cryopreservation of goat semen

AMIT SHARMA; PANKAJ SOOD; JITENDRA KUMAR CHAUDHARY

doi:10.56093/ijans.v90i4.104197

Authors

AMIT SHARMA CSK Himachal Pradesh Krishi Vishvavidyalya, Palampur, Himachal Pradesh 176 062 India
PANKAJ SOOD CSK Himachal Pradesh Krishi Vishvavidyalya, Palampur, Himachal Pradesh 176 062 India
JITENDRA KUMAR CHAUDHARY CSK Himachal Pradesh Krishi Vishvavidyalya, Palampur, Himachal Pradesh 176 062 India

https://doi.org/10.56093/ijans.v90i4.104197

Keywords:

Egg yolk, Extender, Gaddi goats, Semen cryopreservation

Abstract

Gaddi goats are important livestock species of Himachal Pradesh, India. The sensitivity to cryopreservation varies among different species as also between animals of same species. Ejaculates (180) from 11 adult Gaddi bucks aged between 1.1 to 4.5 years (2.16±0.36 years), weighing 31–57 kg (39.1±2.82 kg) were collected using artificial vagina and selected on basis of standard quality parameters. The ejaculates were extended in Tris citrate egg yolk extender containing 6% Glycerol with varying concentrations of egg yolk (EY; 5, 10, 15 and 20%) to maintain a concentration of 150 × 106 sperms/straws. Filled and sealed straws were equilibrated at 5°C for 4 h followed by vapour freezing of straws for 7 min at 4 cm above the liquid nitrogen and finally plunged into liquid nitrogen. The representative straws from each ejaculates were thawed at 37°C for 30 sec, 24 h post incubation to compare the progressive motility, viability, morphological abnormalities and HOST reactive sperms in between different EY concentrations along with per cent change due to the processing. The data was analyzed using package R version 3.4.3. Post thaw progressive motility (35.18±0.87) and viability (45.26±1.32) was higher with least per cent change due to processing (52.03 and 40.12) in 10% EY than other EY concentrations. Absolute average values of morphological abnormalities, were least in 10% EY (7.93±0.28) than 20, 15 and 5% EY (11.42±0.67, 10.84±0.53 and 8.39±0.35), respectively. The absolute average values of HOST did not differ between 15, 10 and 5% (59.96±1.93, 52.48±1.43 and 59.07±2.18) EY, all of which were higher than 20% (42.57±4.20) EY concentrations. In conclusion, extender containing 10% EY was best with respect to progressive motility and viability for Gaddi goat semen cryopreservation.

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