Effect of systemic inflammatory response syndrome (SIRS) on prostaglandin metabolite and oxidative stress in canine pyometra

L KIPJEN SINGH; M K PATRA; G K MISHRA; A C SAXENA; U K DE; S K SINGH; H KUMAR; N KRISHNASWAMY

doi:10.56093/ijans.v90i4.104199

Authors

L KIPJEN SINGH ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
M K PATRA ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
G K MISHRA ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
A C SAXENA ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
U K DE ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
S K SINGH ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
H KUMAR ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
N KRISHNASWAMY ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v90i4.104199

Keywords:

Dog, PGFM, Pyometra, SIRS

Abstract

Pyometra, either open or closed cervix, inevitably progresses to systemic inflammatory response syndrome (SIRS), if ovariohysterectomy is not done timely. The aim of the study was to investigate the effect of pyometra led SIRS on certain serum biochemical and prostaglandin metabolite, 13,14-dihydro-15-keto PGF2 alpha (PGFM) and oxidative stress indices in the canine. The pyometra positive bitches were categorized as SIRS+ (n=29) and SIRS- (n =16) based on temperature (<100.5 or >102.5°F), respiration rate (>20/min), heart rate (>102/min), and total leukocytes count (<6×103 or >16×103 per μL). The SIRS+ bitches showed hypoalbuminemia, hyperglobulinemia, elevated blood urea nitrogen and creatinine, decreased super oxide dismutase (SOD) activity with moderate increase in the lipid peroxidation. Further, the SIRS+ bitches had significantly higher serum PGFM concentration (6.83±0.7 vs. 4.12±0.4 ng/mL) than SIRS- and the level was influenced by cervical patency. It was concluded that elevated serum PGFM along with hyperglobunemia, blood urea nitrogen, creatinine would be useful in diagnosis and monitoring of pyometra led SIRS in bitch.

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References

Acke E, Money C T and Jones B R. 2003. Estrogen toxicity in a dog. Irish Veterinary Journal 56: 465–68.

Benzie F F and Strain J J. 1999. Ferric reducing/ antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology 299: 15–23. DOI: https://doi.org/10.1016/S0076-6879(99)99005-5

Bigliardi E, Parmigiani E, Cavirani S, Luppi A, Bonati L and Corradi A. 2004. Ultrasonography and cystic hyperplasia– pyometra complex in the bitch. Reproduction in Domestic Animals 39(3): 136–40. DOI: https://doi.org/10.1111/j.1439-0531.2004.00489.x

Dabrowski R, Wawron W and Kostro K. 2007. Changes in CRP, SAA and haptoglobin produced in response to ovariohysterectomy in healthy bitches and those with pyometra. Theriogenology 67(2): 321–27. DOI: https://doi.org/10.1016/j.theriogenology.2006.07.019

De Bosschere H, Ducatelle R and Tshamala M. 2003. Uterine oestrogen and progesterone receptor expression in experimental pyometra in the bitch. Journal of Comparative Pathology 128(2–3): 99–106. DOI: https://doi.org/10.1053/jcpa.2002.0610

De Bosschere H, Ducatelle R and Tshmala M. 2002. Is mechanically induced cystic endometrial hyperplasia (CEH) a suitable for study of spontaneously occurring CEH in the uterus of the bitch? Reproduction in Domestic Animals 37(3): 152–57. DOI: https://doi.org/10.1046/j.1439-0531.2002.00358.x

Dhaliwal G K, Wray C and Noakes D. 1998. Uterine bacterial flora and uterine lesions in bitches with cystic endometrial hyperplasia (pyometra). Veterinary Record 143(21): 659–61.

Egenvall A, Hagman R, Bonnett B N, Hedhammar A, Olson P and Lagerstedt A S. 2001. Breed risk of pyometra in insured dogs in Sweden. Journal of Veterinary Internal Medicine 15(6): 530– 38. DOI: https://doi.org/10.1111/j.1939-1676.2001.tb01587.x

Enginler S O, Ates A, Diren Sýgýrcý B, Sontas B H, So¨nmez K, Karacam E, Ekici H, Evkuran Dal G and Gurel A. 2014. Measurement of C-reactive protein and Prostaglandin F2 metabolite concentrations in differentiation of canine pyometra and cystic endometrial hyperplasia/mucometra. Reproduction in Domestic Animals 49(4): 641–47. DOI: https://doi.org/10.1111/rda.12340

Fransson B A, Lagerstedt A S, Bergstrom A, Hagman R, Park J S, Chew B P, Evans M A and Ragle C A. 2007. C-reactive protein, tumor necrosis factor and interleukin-6 in dogs with pyometra and SIRS. Journal of Veterinary Emergency and Critical Care 17(4): 373–81. DOI: https://doi.org/10.1111/j.1476-4431.2006.00203.x

Hagman R, Kindahl H, Fransson B A, Bergstrom A, Strom Holst B and Lagerstedt A S. 2006a. Differentiation between pyometra and cystic endometrial hyperplasia / mucometra in bitches by prostaglandin F2metabolite analysis. Theriogenology 66(2): 198–06. DOI: https://doi.org/10.1016/j.theriogenology.2005.11.002

Hagman R, Kindahl H, and Lagerstedt A S. 2006b. Pyometra in bitches induces elevated plasma endotoxin and prostaglandin F2 alpha metabolite levels. Acta Veterinaria Scandinavica 47(1): 55–67. DOI: https://doi.org/10.1186/1751-0147-47-55

Hagman R, Lagerstedt A S, Hedhammar A and Egenvall A. 2011. A breed-matched case control study of potential risk-factors for canine pyometra. Theriogenology 75(7): 1251–57. DOI: https://doi.org/10.1016/j.theriogenology.2010.11.038

Hagman R, Ronnberg E and Pejler G. 2009. Canine uterine bacterial infection induces up-regulation of proteolysis related genes and down regulation of homeobox and zinc finger factors. PLoS ONE 4e: 8039. DOI: https://doi.org/10.1371/journal.pone.0008039

Hagman R. 2016. Canine pyometra: what is new? Reproduction in Domestic Animals 51(Suppl. 3): 1–5.

Hauptman J C, Walshaw R and Olivier N B. 1997. Evaluation of the sensitivity and specificity of diagnostic criteria for sepsis in dogs. Veterinary Surgery 26(5): 393–97. DOI: https://doi.org/10.1111/j.1532-950X.1997.tb01699.x

Jitpean S. 2015. ‘Predictive markers and risk factors in canine pyometra’. PhD Thesis, Swedish University of Agricultural Sciences, Uppsala. Sweden.

Johnston S D, Kustritz M V R and Olson P N S. 2001. Disorders of the canine uterus and uterine tubes (oviducts), pp. 206–24. Canine and Feline Theriogenology. (Ed) Kersey R. WB Saunders Co. Philadelphia.

Karlsson I, Hagman R, Johannisson A, Wang L, Karlstam E and Wernersson S. 2012. Cytokines as immunological markers for systemic inflammation in dogs with pyometra. Reproduction in Domestic Animals 47: 337–41. DOI: https://doi.org/10.1111/rda.12034

Kaymaz M, Baºtan A and Erunal N. 1999. Kopeklerde CEHPyometra kompleksinin tanýsýnda labarotuvar bulgularýnýn kullanýlmasý. Turkish Journal of Veterinary and Animal Sciences 23: 127–33.

Krekeler N, Marenda M S, Browning G F, Holden K F, Charles J A and Wright P J. 2012. Uropathogenic virulence factor FimH facilitates binding of uteropathogenic Escherichia coli to canine endometrium. Comparative Immunology, Microbiology and Infectious Diseases 35(5): 461–7. DOI: https://doi.org/10.1016/j.cimid.2012.04.001

Maddens B, Heiene R, Smets P, Svensson M, Aresu L, Van der Lugt J, Daminet S and Meyer E. 2011. Evaluation of kidney injury in dogs with pyometra based on proteinuria, renal histomorphology, and urinary biomarkers. Journal of Veterinary Internal Medicine 25(5): 1075–83. DOI: https://doi.org/10.1111/j.1939-1676.2011.0772.x

Madesh M and Balasubramanian K A. 1998. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian Journal of Biochemistry and Biophysics 35(3): 184– 88.

Melih U, Tek C, Gunduzi M C, Sabuncu A, Senunver A, Bagcigil F A and Bakirel T. 2012. Optimum timing for operation in bitches with pyometra related to endotoxemia. Turkish Journal of Veterinary and Animal Sciences 36(1): 35–42.

Sato J, Yasuda J, Muraoka N, Sato R, Tomizawa N, Miyake Y, Okada K and Naito Y. 2002. High level of serum alkaline phosphatase activity and isoenzymes in cases of canine pyometra. Journal of the Japan Veterinary Medical Association 55: 367–70. DOI: https://doi.org/10.12935/jvma1951.55.367

Silva E, Leitao S, Ferreira-Dias G, Cost L L and Mateus L. 2009. Prostaglandin synthesis genes are differentially transcripted in normal and pyometra endometrial of bitches. Reproduction in Domestic Animals 44: 200–03. DOI: https://doi.org/10.1111/j.1439-0531.2009.01393.x

Singh L K, Patra M K, Mishra G K, Singh V, Upmanyu V, Saxena A C, Das G K, Singh S K, Kumar H and Krishnaswamy N. 2018. Endometrial transcripts of proinflammatory cytokine and enzymes in prostaglandin synthesis are upregulated in the bitches with atrophic pyometra. Veterinary Immunology and Immunopathology 205: 65–71. DOI: https://doi.org/10.1016/j.vetimm.2018.10.010

Sugiura K, Nishikawa M, Ishiguro K, Tajima T, Inaba M and Torii R. 2004. Effect of ovarian hormones on periodical changes in immune resistance associated with estrous cycle in the Beagle bitch. Immunobiology 209(8): 619–27. DOI: https://doi.org/10.1016/j.imbio.2004.09.003

Suleiman S A, Ali ME, Zaki Z M S, El Malik E M A and Nasr M A. 1996. Lipid peroxidation and human sperm motility: protective role of vitamin E. Journal of Andrology 17(5): 530– 37.

Szczubial M, Kankofer M, Bochniarz M and Dabrowski R. 2015. Effects of ovariohysterectomy on oxidative stress markers in female dogs. Reproduction in Domestic Animals 50(3): 393– 99. DOI: https://doi.org/10.1111/rda.12501