Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloes


Abstract views: 90 / PDF downloads: 48

Authors

  • P M KEKAN Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
  • S D INGOLE Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
  • A S NAVEKAR Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
  • S V BHARUCHA Bombay Veterinary College, Mumbai, Maharashtra 400 012 India
  • S D KHARDE Bombay Veterinary College, Mumbai, Maharashtra 400 012 India

https://doi.org/10.56093/ijans.v90i4.104201

Keywords:

Anestrous, Anti-Mulleian hormone, Buffaloes, Cattle, Histopathology, Ovary

Abstract

Present study was undertaken to assess AMH concentration in serum and histopathological study of AFC in buffalo ovaries. Total 30 pair of ovary were collected in 10% Neutral Buffer Formalin. Blood samples were also collected before slaughtering the buffalo animals for estimation of AMH concentration. Before processing, 10 serial sections of each pair of ovary was done for histopathological examinations. All the serial sections of the ovaries were processed as per the standard procedure. On the basis of histopathological examinations and follicle count, animals were categorized as cyclic and anestrus. Out of 30 pairs of ovary, 18 pairs were identified as anestrus and 12 as cyclic. The mean number of primordial, antral and growing ovarian follicles count of cyclic buffaloes were 0.42±0.07, 0.15±0.03 and 4.47±0.03 and 0.49±0.02, 0.16±0.03 and 0.41±0.06 for anestrus buffaloes, respectively. Growing follicles were significantly higher in cyclic as compared to anestrous buffaloes. However, the difference of primordial and antral follicle count remained non-significant in cyclic and anestrous buffaloes. Whereas, AMH concentration in cyclic buffaloes (273.50±48.52) was significantly higher than anestrous buffaloes (79.40±7.80). Therefore, it is concluded that, anti-mullerian hormone is an endocrine marker and can predict the fertility of animal. Also, follicular count is positively correlated with AMH concentration.

Downloads

Download data is not yet available.

References

Baldrighi J M, Filho M F S, Batista E O S, Lopes R N V R, Visintin J A, Baruselli P S and Assumpcao M E O A. 2014. Anti-mullerian hormone concentration and antral ovarian follicle population in Murrah heifers compared to Holstein and Gyr kept under the same management. Reproduction in Domestic Animal 49: 1015–20. DOI: https://doi.org/10.1111/rda.12430

Cate R L, Mattaliano R J, Hession C, Tizard R, Farber N M, Cheung A, Ninfa E G, Frey A Z, Gash D J, Chow E P, Fisher R A and Bertonis J M. 1986. Isolation of the bovine and human genes for mullerian inhibiting substance and expression of the human gene in animal cells. Cell 45: 685–98. DOI: https://doi.org/10.1016/0092-8674(86)90783-X

Durlinger A L, Gruijters M J, Kramer P, Karels B, Ingraham H A, Nachtigal M W, Uilenbroek J T, Grootegoed J A and Themmen A P. 2002. Anti-Mullerian hormone inhibits initiation of primordial follicle growth in the mouse ovary. Endocrinology 143: 1076–84. DOI: https://doi.org/10.1210/endo.143.3.8691

Guerreiro B M, Batista E O S, Vieira L M, Sa Filho M F, Rodrigues C A, Castro Netto A, Silveira C R A, Bayeux B M, Dias E A R, Monteiro F M, Accorsi M, Lopes R N V R and Baruselli P S. 2014. Plasma anti-mullerian hormone: An endocrine marker for in vitro embryo production from Bos taurus and Bos indicus donors. Domestic Animal Endocrinology 49: 96–104. DOI: https://doi.org/10.1016/j.domaniend.2014.07.002

Kevenaar M E, Meerasahib M F, Kramer P, Brigitte van de Lang- Born M N, Frank de Jong H, Groome N P, Themmen A P N and Visser J A. 2006. Serum anti-mullerian hormone levels reflect the size of the primordial follicle pool in mice. Endocrinology 147(7): 3228–34. DOI: https://doi.org/10.1210/en.2005-1588

Modina S C, Tessaro I, Lodde V, Franciosi F, Corbani D and Luciano A M. 2013. Reductions in the number of mid-sized antral follicles are associated with markers of premature ovarian senescence in dairy cows. Reproduction Fertility and Development 1–10. DOI: https://doi.org/10.1071/RD12295

Morotti F, Barreiros T R R, Machado F Z, González S M, Marinho L S R and Seneda M M. 2015. Is the number of antral follicles an interesting selection criteria for fertility in cattle. Animal Reproduction 12(3): 479–86.

Rico C, Fabre S, Medigue C, di Clemente N, Clement F, Bontoux M, Touze J L, Dupont M, Briant E, Remy B, Beckers J F and Monniaux D. 2009. Anti-Mullerian hormone is an endocrine marker of ovarian gonadotropin responsive follicles and can help to predict superovulatory responses in the cow. Biology of Reproduction 80: 50–59. DOI: https://doi.org/10.1095/biolreprod.108.072157

Rico C, Medigue C, Fabre S, Jarrier P, Bontoux M, Clement F and Monniaux D. 2011. Regulation of anti-mullerian hormone production in the cow: A multiscale study at endocrine, ovarian, follicular, and granulosa cell levels. Biology of Reproduction 84: 560–71. DOI: https://doi.org/10.1095/biolreprod.110.088187

Rico C, Drouilhet L, Salvetti P, Dalbiès-Tran R, Jarrier P, Jean- Luc T, Pillet E, Ponsart C, Fabre S and Monniaux D. 2012. Determination of anti-mullerian hormone concentrations in blood as a tool to select Holstein donor cows for embryo production: from the laboratory to the farm. Reproduction Fertility and Development 24(7): 932–44. DOI: https://doi.org/10.1071/RD11290

Visser J A, de Jong F H, Laven J S and Themmen A P. 2006. Anti- Mullerian hormone: A new marker for ovarian function. Reproduction 131: 1–9. DOI: https://doi.org/10.1530/rep.1.00529

Downloads

Submitted

2020-09-01

Published

2020-09-01

Issue

Section

Short-Communication

How to Cite

KEKAN, P. M., INGOLE, S. D., NAVEKAR, A. S., BHARUCHA, S. V., & KHARDE, S. D. (2020). Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloes. The Indian Journal of Animal Sciences, 90(4), 578-580. https://doi.org/10.56093/ijans.v90i4.104201
Citation