Association of polymorphic variant of exons 6 and 11 of lactoferrin gene with mastitis in Murrah buffalo

KRISHANENDER DINESH; ARCHANA VERMA; I D GUPTA; S K DASH

doi:10.56093/ijans.v90i4.104206

Authors

KRISHANENDER DINESH ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
ARCHANA VERMA ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
I D GUPTA ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
S K DASH ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v90i4.104206

Keywords:

Lactoferrin gene, Murrah buffalo, PCR-RFLP, SNP

Abstract

Lactoferrin is one of the important candidate genes for mastitis resistance in dairy animals. The gene is located on Bos taurus autosome (BTA) 22 and consists of 17 exons spanning over 34.5 kb of genomic DNA. The present study was undertaken to identify allelic variant in exons 6 and 11 of lactoferrin gene and to analyze association with incidence of clinical mastitis in Murrah buffalo. The amplification of exons 6 and 11 of lactoferrin gene yielded 301 and 131 bp amplicon size. Comparison of nucleotide sequence of exonic region of lactoferrin gene with Bos taurus (NCBI accession number AC_000179.1) revealed 6 mutations; among them 3 were in coding DNA sequence and remaining 3 were in flanking intronic region. All these mutations were found in exon 6 and synonymous in nature without affecting the sequence of amino acid. PCR-restriction fragment length polymorphism (RFLP) analysis of 301 bp amplicon using FokI restriction enzyme exhibited polymorphic pattern with two genotypes (AA and AB) with respective frequency of 0.625 and 0.375. The frequencies of two alleles, A and B were estimated as 0.81 and 0.19 respectively. RE Hpy188I and HinfI used for digestion of exon 11 had exhibited monomorphic pattern. The chi-square (χ²) analysis revealed significant association between incidence of clinical mastitis and genetic variant of exon 6 and animals with AA genotype were found to be less susceptible to mastitis. The findings indicate potential scope for incorporation of lactoferrin gene in selection and breeding of Murrah buffaloes for improved genetic resistance to mastitis.

Downloads

Download data is not yet available.

References

Bukhari Saba, Das A K, Kumar N, Raghuwanshi P, Taggar R K, Chakraborty D, Vohra V and Gupta P. 2015. Genetic polymorphism of promoter region of lactoferrin gene and its association with mastitis resistance in Jersey crossbred cattle. Indian Journal of Animal Research 49(2): 165–67. DOI: https://doi.org/10.5958/0976-0555.2015.00109.0

Dinesh K, Verma A, Gupta I D, Thakur Y P, Verma N and Arya A. 2015. Identification of polymorphism in exons 7 and 12 of Lactoferrin gene and its association with incidence of clinical mastitis in Murrah buffalo. Tropical Animal Health Production. DOI 10.1007/s 11250-015-0765-z. DOI: https://doi.org/10.1007/s11250-015-0765-z

Joshi S and Gokhale S. 2006. Status of mastitis as an emerging disease in improved and periurban dairy farms in India. Annals of the New York Academy of Sciences 1081(1): 74–83. DOI: https://doi.org/10.1196/annals.1373.007

Kathiravan P, Kataria R S, Mishra B P, Dubey P K, Selvakumar M and Tyagi N. 2009. Seven novel single nucleotide polymorphisms identified within river buffalo (Bubalus bubalis) lactoferrin gene. Tropical Animal Health Production 42: 1021–26. DOI: https://doi.org/10.1007/s11250-009-9516-3

Kathiravan P, Kataria R S, Mishra B P, Tyagi N and Selvakumar M. 2010. Sequence characterization and polymorphism detection in exons 6, 7 and 13 of the Bubaline lactoferrin gene. Buffalo Bulletin 29(3).

Kimber I, Cumberbatch M, Dearman D R, Headon D R, Bhushan M and Griffiths C E M. 2002. Lactoferrin: influences on Langerhans cells, epidermal cytokines, and cutaneus inflammation. Biochemistry and Cell Biology 80: 103–07. DOI: https://doi.org/10.1139/o01-227

Li G H, Zhang Y, Sun D X and Li N. 2004. Study on the polymorphism of bovine lactoferrin gene and its relationship with mastitis. Animal Biotechnology 15: 67–76. DOI: https://doi.org/10.1081/ABIO-120037899

Rahmani S F. 2011. ‘Studies on bovine lactoferrin gene and its association with mastitis in cattle.’Ph.D. Thesis, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka.

Raja K N. 2007. Characterization of bovine lactoferrin gene and its polymorphism in Sahiwal cattle. PhD Thesis, NDRI, Karnal.

Rodriguez Nancy C, Lopez Albeiro H and Julia Echeverri Z. 2013. Population genetic structure of bovine lactoferrin gene in Holstein breed of Antioquia Department. Revista MVZ Córdoba 18(1): 3355–61. DOI: https://doi.org/10.21897/rmvz.198

Sambrook J and Russell D W. 1989. 3rd edition. Cold Spring Harbor Laboratory Press, New York 6.1–6.62.

Sanchez L, Calvo M and Brock J H. 1992. Biological role of lactoferrin. Archives of Disease in Childhood 67: 657–61. DOI: https://doi.org/10.1136/adc.67.5.657

Sender G, Korwin-Kossakowska A, Galal K and Prusak B. 2006. Ocena wp³ywu polimorfizmu wybranych genów na wystêpowanie mastitis u krów. In Polish. Medycyna Weterynaryjna 62: 563–65.

Seyfert H M and Kuhn C. 1994. Characterization of a first bovine lactoferrin gene variant based on an EcoRI polymorphism. Animal Genetics 25: 54. DOI: https://doi.org/10.1111/j.1365-2052.1994.tb00451.x

Sharifzadesh A and Doosti A. 2011. Study of lactoferrin gene polymorphism in Iranian Holstein cattle using PCR-RFLP technique. Global Veterinaria 6(6): 530–36.

Singh AP, Ramesha K P, Isloor S, Divya P, Arya A and Mir M A. 2016. Sequence characterization and polymorphism detection in lactoferrin gene of Deoni (Bos indicus) cattle. Indian Journal of Animal Research 50(4): 455–59. DOI: https://doi.org/10.18805/ijar.6711

Snedecor G W and Cochran W G. 1994. Statistical Methods. 8th ed. State University Press, USA.

Srubarova P and Dovrak J. 2010. Association between Lactoferrin gene polymorphism and bovine mammary gland inflammation. Teng C T. 2002. Lactoferrin gene expression and regulation: an overview. Biochemistry and Cell Biology 80: 7–16. DOI: https://doi.org/10.1139/o01-215

Zhao C, He G, Wan Y and Zhang Z. 2009. Polymorphism of lactoferrin gene with PCR-RFLP and its association with subclinical mastitis in dairy cows. Modern Applied Sciences 3: 144–46. DOI: https://doi.org/10.5539/mas.v3n2p144