Genetic polymorphism in MAP1B gene associated with conception rate in Holstein Friesian crossbred breeding bulls


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Authors

  • ARPAN UPADHYAY ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • ATISH KUMAR CHAKRAVARTY ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • SHACHINANDAN DE ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • A SAKTHIVEL SELVAN ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • PUSHPRAJ SHIVAHRE ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • ASHOK KUMAR GUPTA ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
  • AVTAR SINGH ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v90i4.104227

Keywords:

Conception rate, Karan Fries bulls, MAP1B gene, SNP, T-ARMS PCR

Abstract

The microtubule associated protein 1 B (MAP1B) gene is one of the candidate genes which was found to be strongly associated with the sire conception rate. In the present study, identification of single nucleotide polymorphisms (SNPs) in the targeted region of MAP1B gene and their effect on conception rate (CR) in Karan Fries (KF) breeding bulls were sought. A tetra-primer ARMS PCR technique was adapted to genotype an SNP, g. 3,066A>G located on intron 1 of MAP1B gene. The relationship between the CR of KF breeding bulls and polymorphic locus of MAP1B gene was assessed. Genotyping of the targeted region revealed three kinds of genotype (AA, AG and GG) with respect to g. 3,066A>G SNP locus. The identified SNP had highly significant (P=0.0009) influence on CR. The KF bulls with AA and AG genotype had comparatively higher CR than the bulls with GG genotype. The identified SNP was significantly associated with CR, thus it may be utilized as a molecular marker for developing marker-assisted selection strategy to enhance the CR in KF cattle population.

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References

Basic Animal Husbandry Statistics. 2015. Ministry of Agriculture and Farmers Welfare, Department of Animal Husbandry, Dairying and Fisheries, New Delhi, Government of India.

Bonilla E and Xu E Y. 2008. Identification and characterization of novel mammalian spermatogenic genes conserved from fly to human. Molecular Human Reproduction 14: 137–42. DOI: https://doi.org/10.1093/molehr/gan002

Li G, Penagaricano F, Weigel K A, Zhang Y, Rosa G and Khatib H. 2012. Comparative genomics between fly, mouse, and cattle identifies genes associated with sire conception rate. Journal of Dairy Science 95(10): 6122–29. DOI: https://doi.org/10.3168/jds.2012-5591

NDDB. 2017. National Dairy Development Board, Anand, Gujarat, INDIA (https://www.nddb.coop/services/animal breeding/animalreproduction/semenproduction)

Panmei A, Gupta A K, Shivahre P R, Bhakat M and Dash S K. 2016. Culling pattern of Karan Fries (crossbred) males in an organised herd. Indian Journal of Animal Research 50(6): 1005–08. DOI: https://doi.org/10.18805/ijar.10770

Queiroz D B, Silva A M, Gutierrez-Ospina G, Porto C S, Grossman G, Petrusz P and Avellar M C. 2006. Cells positive for microtubule associated protein 1B (MAP 1B) are present along rat and human efferent ductules and epididymis. Cell Tissue Research 325: 125–33. DOI: https://doi.org/10.1007/s00441-005-0108-x

SAS Institute 2012. SAS statistics, Cary, NC. Version 9.3. Yeh F C, Yang R C and Boyle T. 1999. POPGENE VERSION 1.31: Microsoft Window-based free Software for Population Genetic Analysis, 1999. https://sites.ualberta.ca/~fyeh/popgene_download.html.

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Submitted

2020-09-01

Published

2020-09-01

Issue

Section

Short-Communication

How to Cite

UPADHYAY, A., CHAKRAVARTY, A. K., DE, S., SELVAN, A. S., SHIVAHRE, P., GUPTA, A. K., & SINGH, A. (2020). Genetic polymorphism in MAP1B gene associated with conception rate in Holstein Friesian crossbred breeding bulls. The Indian Journal of Animal Sciences, 90(4), 658-660. https://doi.org/10.56093/ijans.v90i4.104227
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