Characterization of non-typhoidal Salmonella from poultry in Punjab, India

SARMISTHA KAR; RANDHIR SINGH; SIMRAN PREET KAUR; PARMINDER SINGH; J P S GILL

doi:10.56093/ijans.v90i5.104602

Authors

SARMISTHA KAR Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
RANDHIR SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
SIMRAN PREET KAUR Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
PARMINDER SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
J P S GILL Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India

https://doi.org/10.56093/ijans.v90i5.104602

Keywords:

Antimicrobial susceptibility, PCR, PFGE, Poultry, Salmonella

Abstract

Present study was done to determine the prevalence, virulence and antimicrobial spectrum of identified serotypes. A total of 693 samples, comprising 585 poultry fecal samples (420 from layer, 120 from broiler and 45 from backyard poultry), 54 each poultry feed and farm water were collected from 31 poultry farms of three districts of Punjab. A total of 6 Salmonella isolates were obtained from these samples. Out of 585 fecal samples, 6 (1.02%) were positive for Salmonella. Out of 31 farms, 2 (6.45%) farms were positive for Salmonella. Out of 6 Salmonella isolates, four isolates from one farm were serotype Salmonella IIIa, 35: z24: z23 and two Salmonella isolates from another farm were untypable and also carried spvC gene. Isolates showed resistance to gentamicin (2/6, 33.3%), co-trimoxazole (1/6, 16.7%). Antibiotics ampicillin, tetracycline, ciprofloxacin, chloramphenicol and enrofloxacin were effective against all the isolates. On PFGE analysis, four isolates from one farm were clustered in two clusters and two isolates from other farm were similar and clustered together. Based on the results we can say that drug resistant Salmonella is present in poultry flock in Punjab.

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References

Akhtar F, Hussain I, Khan A and Rahman S U. 2009. Prevalence and antibiogram studies of Salmonella enteritidis isolated from human and poultry sources. Pakistan Veterinary Journal 30(1): 25–28

Al-Abadi I K M and Mayah A A S Al. 2012. Isolation and identification of Salmonella spp. from chicken and chicken environment in Basrah province. Iraqi Poultry Sciences Journal 7(1): 88–99

Balakrishnan S, Sangeetha A and Dhanalakshmi M. 2018. Prevalence of Salmonella in chicken meat and its slaughtering place from local markets in Orathanadu, Thanjavur district, Tamil Nadu. Journal of Entomology and Zoology Studies 6(2): 2468–71.

Bhuvaneswari M, Shanmughapriya S and Natarajaseenivasan K. 2015. Prevalence of multidrug-resistant (MDR) Salmonella enteritidis in poultry and backyard chicken from Tiruchirappalli, India. Microbiology Journal 5(2): 28–35 DOI: https://doi.org/10.3923/mj.2015.28.35

Boonmar S, Bangtrakulnonth A, Pornrunangwong S, Terajima J, Watanabe H, Kaneko K and Ogawa M. 1998. Epidemiological analysis of Salmonella Enteritidis isolates from humans and broiler chickens in Thailand by phage typing and pulsed-field gel electrophoresis. Journal of Clinical Microbiology 36(4): 971–74. DOI: https://doi.org/10.1128/JCM.36.4.971-974.1998

Centers for Disease Control and Prevention (CDC). 1999. Reptileassociated salmonellosis-selected states, 1996–1998. Morbidity and Mortality Weekly Report 48: 1009–13.

Chu C and Chiu C H. 2006. Evolution of the virulence plasmids of non-typhoid Salmonella and its association with antimicrobial resistance. Microbes and Infection 8: 1931–36 DOI: https://doi.org/10.1016/j.micinf.2005.12.026

Das D, Panda S K, Jena B, Mallick H N and Sahu R K. 2017. Conventional and molecular detection of Salmonella species in backyard poultry of Odisha state in India. Journal of Entomology and Zoology Studies 5(6): 837–40.

El-Tayeb M A, Ibrahim A S S, Al-Salamah A A, Almaary K S and Elbadawi Y B. 2017. Prevalence, serotyping and antimicrobials resistance mechanism of Salmonella enterica isolated from clinical and environmental samples in Saudi Arabia. Brazilian Journal of Microbiology 48(3): 499–508. DOI: https://doi.org/10.1016/j.bjm.2016.09.021

Fanelli M J, Sadler W W, Franti C E and Brownell J R. 1991. Localization of Salmonellae within the intestinal tract of chickens. Avian Diseases 15(2): 366–75. DOI: https://doi.org/10.2307/1588708

Finlay B B and Falkow S. 1989. Microreview: Salmonella as an intracellularparasite. Molecular Microbiology 3: 1833–41. DOI: https://doi.org/10.1111/j.1365-2958.1989.tb00170.x

Guiney D, Fang F, Krause and Libby S. 1994. Plasmid mediated virulence genes in non-typhoid Salmonella serovaras. FEMS Microbiology Letters 124: 1–9. DOI: https://doi.org/10.1111/j.1574-6968.1994.tb07253.x

Gulig P A. 1990. Virulence plasmids of S. Typhimurium and other salmonellae. Microbial Pathogenesis 8: 3–11. DOI: https://doi.org/10.1016/0882-4010(90)90003-9

Hall M L and Rowe B. 1992. Salmonella arizonae in the United Kingdom from 1966 to 1990. Epidemiology and Infection 108: 59–65. DOI: https://doi.org/10.1017/S0950268800049505

Jarolmen H, Sairk R and Langworth B. 2008. Effect of chlortetracycline feeding on the Salmonella reservoir in chickens. Journal of Applied Microbiology. 40: 153–61. DOI: https://doi.org/10.1111/j.1365-2672.1976.tb04163.x

Li X, Liu L, Li Q, Xu G and Zheng J. 2018. Salmonella contamination in layer farms in China: Detection and genetic analysis. Journal of Poultry Science 55: 1–9. DOI: https://doi.org/10.2141/jpsa.0160144

Marshall B M and Levy S B. 2011. Food Animals and Antimicrobials: Impacts on Human Health. Clinical Microbiological Review 24: 718–33. DOI: https://doi.org/10.1128/CMR.00002-11

Mir I A, Kashyap S K and Maherchandani S. 2015. Isolation, serotype diversity and antibiogram of Salmonella enterica isolated from different species of poultry in India. Asian Pacific Journal of Tropical Biomedicine 5(7): 561–67. DOI: https://doi.org/10.1016/j.apjtb.2015.03.010

Mir I A, Wani S A, Hussain I, Qureshi S D, Bhat M A and Nishikawa Y. 2010. Molecular epidemiology and in vitro antimicrobial susceptibility of Salmonella isolated from poultry in Kashmir. OIE Scientific and Technical Review 3: 677–86. DOI: https://doi.org/10.20506/rst.29.3.2011

Orji M U, Onuigbo H C and Mbata T I. 2004. Isolation of Salmonella from poultry droppings and other environmental sources in Awka. Nigeria. International Journal of Infectious Diseases 9: 86–90. DOI: https://doi.org/10.1016/j.ijid.2004.04.016

Scallan E, Hoekstra R M, Angulo F J, Tauxe R V, Widdowson M A, Roy S L, Jones J L and Griffin P M. 2011. Foodborne illness acquired in the United States—major pathogens. Emerging Infectious Disease 17: 7–18. DOI: https://doi.org/10.3201/eid1701.P11101

Schroter M P, Hofmann R J, Speicher A, Laufs R and Mack D. 2004. Pet snakes as a reservoir for Salmonella enterica subsp. diarizonae (serogroup IIIb): a prospective study. Applied and Environmental Microbiology 70: 613–15. DOI: https://doi.org/10.1128/AEM.70.1.613-615.2004

Silbergeld E K, Graham J and Price L B. 2008. Industrial food animal production, antimicrobial resistance and human health. Annual Reviews of Public Health 29: 151–69. DOI: https://doi.org/10.1146/annurev.publhealth.29.020907.090904

Sudhanthirakodi S, Jain P, Chattopadhyay U K and Dutta S. 2016. Non-typhoidal Salmonella isolates from livestock and food samples, Kolkata, India. Journal of Microbiology and Infectious Diseases 6(3): 113–20. DOI: https://doi.org/10.5799/jmid.vi.328974

Suresh T, Hatha A A M, Harsha H T and Lakshmanaperumalsamy P. 2010. Prevalence and distribution of Salmonella serotypes in marketed broiler chickens and processing environment in Coimbatore City of southern India. Food Research International 44: 823–25. DOI: https://doi.org/10.1016/j.foodres.2011.01.035

Tiwari R, Singh S P and Singh R. 2014. Study on prevalence of Salmonella serotypes among poultry and cattle in and around Pantnagar. Journal of Veterinary Public Health 12(2): 85–88.

Waterman S H, Juarez G, Carr S J and Kilman L. 1990. Salmonella arizona infections in Latinos associated with rattlesnake folk medicine. American Journal of Public Health 80: 286–89. DOI: https://doi.org/10.2105/AJPH.80.3.286

Weiss S H, Blasé M J, Paleologo F P, Black R E, McWorther A C, Asbury M A, Carter G P, Feldman R A and Brenner D J. 1986. Occurrence and distribution of serotypes of the Arizona subgroup of Salmonella strains in the United States from 1967 to 1976. Journal of Clinical Microbiology 23: 1056–64. DOI: https://doi.org/10.1128/jcm.23.6.1056-1064.1986

Whittam T S and Bergholz T M. 2007. Molecular subtyping, source tracking and food safety, pp. 93–136. Microbial Source Tracking. (Eds.) Domingo J W S and Sadowsky M J. ASM Press, Washington, DC. DOI: https://doi.org/10.1128/9781555815769.ch4