Differential expression of TGF-β and IL-1R2 genes during endometritis infection in Egyptian buffalo
Abstract views: 140 / PDF downloads: 63
https://doi.org/10.56093/ijans.v90i5.104611
Keywords:
Buffalo, Egyptian, Endometritis, IL-1R2, QT-PCR, TGF-βAbstract
Contamination of the uterine lumen with bacteria occurred in bovid within the first week after parturition. The bacterial infection causes the persistent inflammation in the endometrium leading to the infertility and huge economical loss in animal production. TGF-β and IL-1R2 genes are involved in innate immune recognition of pathogens and the inflammatory response. This study aimed to compare the expression of these two genes in uteri samples of endometritis-infected and apparently healthy buffaloes using QT-PCR. The uteri samples were collected from endometritis-infected and normal buffaloes. Bacterial examination of uteri from endometritis-infected buffaloes showed the presence of bacterial contamination with E. coli, P. Klebsiella pneumonia and/or P. vulgaris. RNA was extracted from uteri of infected and normal animal, and cDNA was synthesized for QT-PCR. Using GAPDH as a housekeeping gene, the gene expression of two tested genes was assessed and the results showed that the expression of TGF-β and IL-1R2 genes was up-regulated in infected animals compared to control by 11.39 and 12.99 folds, respectively and this increase of gene expressions was highly significant. In conclusion, the gene expression assessment of important innate immune genes—like TGF-β and IL-1R2 genes can help to establish new approaches for the improvement of the immune response of buffalo through marker-assisted selection of animals characterized by superior innate immunity system.
Downloads
References
Abdel Aziz K B, Khalil W K B, Mahmoud M S, Hassan N H A, Mabrouk D M and Suarez C E. 2014. Molecular characterization of babesiosis infected cattle: Improvement of diagnosis and profiling of the immune response genes expression. Global Veterinaria 12: 197–206.
Buza J J, Mori Y, Bari A M, Hikono H, Hirayama S, Shu Y and Momotani E. 2003. Mycobacterium avium subsp.
Paratuberculosis infection causes suppression of RANTES, monocyte chemo-attractant protein 1 and tumor necrosis factor alpha expression in peripheral blood of experimentally infected cattle. Infection and Immunity 71: 7223–27. DOI: https://doi.org/10.1128/IAI.71.12.7223-7227.2003
Coussens P M, Verman N, Coussens M A, Elftman M D and McNulty A M. 2004. Cytokine gene expression in peripheral blood mononuclear cells and tissues of cattle infected with Mycobacterium avium subsp. paratuberculosis: Evidence for an inherent pro-inflammatory gene expression pattern. Infection and Immunity 72: 1409–22. DOI: https://doi.org/10.1128/IAI.72.3.1409-1422.2004
Fung J N, Mortlock S, Girling J E, Holdsworth-Carson S J, Teh W T, Zhu Z, Lukowski S W, McKinnon B D, McRae A, Yang J, Healey M, Powell J E, Rogers P A W and Montgomery G W. 2018. Genetic regulation of disease risk and endometrial gene expression highlights potential target genes for endometriosis and polycystic ovarian syndrome. Scientific Reports 8(11424): 1–19. DOI: https://doi.org/10.1038/s41598-018-29462-y
Galdier M, Marcatili A, Cipollaro G, Ero D, Nuzzo I, Bentivoglio C, Galdiero M and Carratelli C R. 1999. Effect of transforming growth factor-on experimental Salmonella typhimurium infection in mice. Infection and Immunity 67: 1432–38. DOI: https://doi.org/10.1128/IAI.67.3.1432-1438.1999
Gilbert R O, Shin S T, Guard C L and Erb H N. 1998. Incidence of endometritis and effects on reproductive performance of dairy cows. Theriogenology 49: 251. DOI: https://doi.org/10.1016/S0093-691X(98)90604-5
Gómez-Laguna J, Rodríguez-Gómez I M, Barranco I, Pallares F J, Salguero F J and Carrasco L. 2012. Enhanced expression of TGFprotein in lymphoid organs and lung, but not in serum, of pigs infected with a European field isolate of porcine reproductive and respiratory syndrome virus. Veterinary Microbiology 158: 187–93. DOI: https://doi.org/10.1016/j.vetmic.2012.02.003
Herath S, Fischer D P, Werling D, Williams E J, Lilly S T, Dobson H, Bryant C E and Sheldon I M. 2006. Expression and function of Toll-like receptor 4 in the endometrial cells of the uterus. Endocrinology 147: 562–70. DOI: https://doi.org/10.1210/en.2005-1113
Herath S, Lilly S T, Santos N R, Gilbert R O, Goetze L, Bryant C E, White J O, Cronin J and Sheldon I M. 2009. Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility. Reproductive Biology and Endocrinology 7: 5. DOI: https://doi.org/10.1186/1477-7827-7-55
Lim A, Steibel J P, Coussens P M, Grooms D L and Bolin S R. 2012. Differential gene expression segregates cattle confirmed positive for bovine Tuberculosis from Antemortem Tuberculosis test-false positive cattle originating from herds free of bovine Tuberculosis. Veterinary Medicine International 12: 1–11. DOI: https://doi.org/10.1155/2012/192926
Livak K J and Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2βCt method. Methods 4: 402–08. DOI: https://doi.org/10.1006/meth.2001.1262
Massagué J. 2012. “TGFβ signalling in context”. Nature Reviews Molecular Cell Biology 13: 616–30. DOI: https://doi.org/10.1038/nrm3434
Peters V A, Joesting J J and Freund G G. 2013. IL-1 receptor 2 (IL-1R2) and its role in immune regulation. Brain, Behavior and Immunity 32: 1–8. DOI: https://doi.org/10.1016/j.bbi.2012.11.006
Sheldon I M, Cronin J, Goetze L, Donofrio G and Schuberth H J. 2009. Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biology of Reproduction 81: 1025–32. DOI: https://doi.org/10.1095/biolreprod.109.077370
Vicky J, Young S F, Ahmad W, Duncan C and Horne A W. 2017. The role of TGF-β in the pathophysiology of peritoneal endometriosis. Human Reproduction Update 23: 548–59. DOI: https://doi.org/10.1093/humupd/dmx016
Wang J, Zhang C, Wei K, Blagosklonov O, Guodong L, Xiaomei L, Mantion G, Vuitton D A, Wen H and Lin R. 2013. TGF- and TGF-/Smad signaling in the interactions between Echinococcus multilocularis and its hosts. PLoS ONE 8: e553. DOI: https://doi.org/10.1371/journal.pone.0055379
Williams E J, Fischer D P, Noakes D E, England G C, Rycroft A, Dobson H and Sheldon I M. 2007. The relationship between uterine pathogen growth density and ovarian function in the postpartum dairy cow. Theriogenology 68: 549–59. DOI: https://doi.org/10.1016/j.theriogenology.2007.04.056
Xu Q Q, Xu P, Zhou J W, Pan T S, Tuo R, Ai K and Yang D Q. 2016. Cloning and expression analysis of pro-inflammatory cytokines, IL-1β and its receptor, IL-1R2, in the Asian Swamp Eel Monopterusalbus. Molecular Biology 50: 671–76. DOI: https://doi.org/10.1134/S0026893316030122
Downloads
Submitted
Published
Issue
Section
License
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.