Effect of season and SNPs of HSP90 and HSP70 genes on the biochemical traits in Indian sheep (Ovis aries) breeds

SANJEEV SINGH; K M SINGH; INDRAJIT GANGULY

doi:10.56093/ijans.v90i6.105012

Authors

SANJEEV SINGH ICAR-National Bureau of Animal Genetics Resources, Karnal, Haryana 132 001 India
K M SINGH ICAR-National Bureau of Animal Genetics Resources, Karnal, Haryana 132 001 India
INDRAJIT GANGULY ICAR-National Bureau of Animal Genetics Resources, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v90i6.105012

Keywords:

Cortisol, HSP genes, Ovis aries, Thyroxine, Triiodothyronine

Abstract

Heat shock proteins (HSPs) are evolutionary conserved family of proteins produced by living cells in response to various biological stresses, including heat shock. Heat stress affects the productivity of the livestock species, which causes severe economic losses to the animal keepers. The present study was undertaken to establish reference baseline values for thyroid (T3 and T4) and adrenal gland (cortisol) hormones in four indigenous sheep breeds (Chokla, Marwari, Magra and Madras Red). The effect of the seasons and genotypes of the HSP90 and HSP70 genes on the functions of the thyroid gland as well as cortisol levels were examined in plasma samples of these sheep breeds. A total of 80 plasma samples (10 animals per breed for the summer and winter season) were analyzed. The least square analysis revealed significant seasonal effect on Triiodothyronine (T3) and Thyroxine (T4). Significantly higher T3 and T4 levels were observed in the winter with a low temperature humidity index (THI) than in summer with high THI. Cortisol was non-significantly higher in the summer than in winter season. Overall, the influence of the breeds on these three parameters was non-significant. Genotypes of SNP1 (HSP90 gene) and SNP2 (HSP70 gene) had significant effect on T3 level. These SNP markers may be useful for identifying animals that are more adaptable to heat stress following a thorough association analysis over a large sample size.

Downloads

Download data is not yet available.

References

Atkinson H C, Wood S A, Kershaw Y M, Bate E and Lightman S L. 2006. Diurnal variation in the responsiveness of the hypothalamic-pituitary-adrenal axis of the male rat to noise stress. Journal of Neuroendocrinology 18: 526–33. DOI: https://doi.org/10.1111/j.1365-2826.2006.01444.x

Benjamin I J and McMillan D R. 1998. Stress proteins: Molecular chaperones in cardiovascular biology and disease. Circulation Research 83: 117–25. DOI: https://doi.org/10.1161/01.RES.83.2.117

Bhimte A, Thakur N, Lakhani N, Yadav V, Khare A and Lakhani P. 2018. Journal of Pharmacognosy and Phytochemistry 7(4): 127–32.

Dong Q and Liu X. 2013. Effect of fear stress in rats on the contents of ACTH, CORT, IL 2, IL 8. Journal of Molecular Diagnosis and Therapy 5: 173–76.

Fialkovicova M, Mardzinova S, Benkova M, Mojzisova J, Gaalova M and Sesztakova E. 2012. Seasonal influence on the thyroid gland in healthy dogs of various breeds in different weights. Acta Veterinaria Brno 81(2): 183–88. DOI: https://doi.org/10.2754/avb201281020183

Freak H C and Oppenheimer J H. 1995. Thermogenesis and thyroid function. Annual Review of Nutrition 15: 263–29. DOI: https://doi.org/10.1146/annurev.nu.15.070195.001403

Gaughan J B, Mader T L, Holt S M, Sullivan M L and Hahn G L. 2010. Heat tolerance of Boran and Tuli crossbred steers. Journal of Animal Science 77: 2398–2405. DOI: https://doi.org/10.2527/1999.7792398x

Helal A, Hashem A L S, Abdel-Fattah M S and El-Shaer. 2010. Effect of heat stress on coat characteristics and physiological responses of balady and Damascus goat in Sinai Egypt. American Eurasian Journal of Agricultural and Environmental Sciences 7(1): 60–69.

Nazifi S, Saeb M, Rowghani E and Kaveh K. 2003.The influences of thermal stress on serum biochemical parameters of Iranian fat-tailed sheep and their correlation with triiodothyronine (T3) thyroxine (T4) and cortisol concentrations. Comparative Clinical Pathology 12: 135–39. DOI: https://doi.org/10.1007/s00580-003-0487-x

Rathwa S D, Vasava A A, Pathan M M, Madhira S P, Patel Y G and Pande A M. 2017. Effect of season on physiological, biochemical, hormonal and oxidative stress parameters of indigenous sheep. Veterinary World 10(6): 650–54. DOI: https://doi.org/10.14202/vetworld.2017.650-654

Sejian V, Indu S and Naqvi S M K. 2013. Impact of short term exposure to different environmental temperature on the blood biochemical and endocrine responses of Malpura ewes under semi-arid tropical environment. Indian Journal of Animal Sciences 83: 1155–60.

Singh K M, Singh S, Ganguly I, Raja K N, Ganguly A, Vekataramanan R, Chopra A and Narula H K. 2017. Association of heat stress protein 90 and 70 gene polymorphism with adaptability traits in Indian sheep (Ovis aries). Cell Stress and Chaperones 22: 675–84. DOI: https://doi.org/10.1007/s12192-017-0770-4

Sivakumar A V N, Singh G and Varshney V P. 2010. Antioxidants supplementation on acid base balance during heat stress in goat. Asian Australasian Journal of Animal Science 23(11): 1462–68. DOI: https://doi.org/10.5713/ajas.2010.90471