Biotin fortification to sperm preparation medium enhances the motility and longevity by reducing lipid peroxidation in cryopreserved sperm

Ravi Ranjan; M KUMAR; S P SINGH; S D KHARCHE; M K SINGH; M S CHAUHAN

doi:10.56093/ijans.v92i8.105045

Authors

Ravi Ranjan CIRG, MAKHDOOM, FARAH, MATHURA, U.P-281122
M KUMAR ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh 281 122 India
S P SINGH ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh 281 122 India
S D KHARCHE ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh 281 122 India
M K SINGH ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh 281 122 India
M S CHAUHAN ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, Uttar Pradesh 281 122 India

https://doi.org/10.56093/ijans.v92i8.105045

Keywords:

Antioxidant, Biotin, Goat, Melonaldehyde, Post thaw semen

Abstract

Biotin supplementation to the sperm cryopreservation medium appears to be beneficial in enhancing the in vitro
sperm survival without affecting the fertilizing ability. Since, biotin is an essential micronutrient, it may be a safer sperm motility enhancing agent. We inferred that the fortification of 3 μM biotin significantly (P<0.05) reduced
the detrimental effects of freezing stress on motility, viability, membrane integrity and acrosome integrity of buck sperm. This particular concentration also showed significant (P<0.05) improvement in protection of sperm membrane by lipid peroxidation. Therefore, biotin may be a promising and safe alternative agent to enhance the post-thaw sperm quality in an ART. However, further studies are required to elucidate the exact molecular mechanism behind biotin-induced sperm motility enhancement.

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Author Biography

Ravi Ranjan, CIRG, MAKHDOOM, FARAH, MATHURA, U.P-281122

SENIOR SCIENTIST, ICAR-CIRG, MAKHDOOM, FARAH, MATHURA, U.P-281122

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