Comparative analysis of intradermal tuberculin test and γ-interferon assay for diagnosis of bovine tuberculosis

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  • S S VYAVAHARE MVSc ScholarDepartment of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand – 388001, Gujarat
  • M K JHALA Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand – 388001, Gujarat
  • B B BHANDERI Assistant Professor & HeadDepartment of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand – 388001, Gujarat


Bovine tuberculosis, ELISPOT, IFN-γ, Tuberculin test


The present study was carried out in and around Anand district of Gujarat with the aim to assess the sensitivity and specificity of IFN-γ assay as compared to intradermal tuberculin test for diagnosis of bovine tuberculosis during the period 2011-12. The prevalence of bovine TB in the cattle was 26.19% by TST and 38.49% by IFN- γ assay. Breed wise, highest prevalence of bovine TB was found in Gir (38.96%), followed by Triple cross (23.95%) and Kankrej (16.45%) by TST; while by IFN- γ assay, highest prevalence was observed in Triple cross (42.70%). Age wise, highest prevalence of bovine TB was found in adults (37.90%) followed by calves (26.66%) and heifers (11.22%) by TST; while by IFN-γ assay, highest prevalence was observed in calves (66.66%) followed by adults (44.35%) and heifers (22.44%). Sex wise, more prevalence of bovine TB was found in males (56.25%) than in females (21.81%) by TST; while by IFN-γ assay, more prevalence was observed in females (39.25%) than in males (31.25%). Sensitivity and specificity of TST in detecting bovine TB were 27.27% and 57.52% respectively, compared to IFN-γ assay. ELISPOT assay showed 34.78% animals were found positive for bovine TB. IFN-γ assay showed better sensitivity in detecting bovine TB at younger age compared to TST, and hence can be useful in timely removal of the infected animals from the herd.


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How to Cite

VYAVAHARE, S. S., JHALA, M. K., & BHANDERI, B. B. (2022). Comparative analysis of intradermal tuberculin test and γ-interferon assay for diagnosis of bovine tuberculosis. The Indian Journal of Animal Sciences, 92(9), 1041–1045.