Molecular typing of fowl adenovirus associated with gizzard erosion in commercial layer grower chicken in Tamil Nadu

S CHITRADEVI; K SUKUMAR; P SURESH; G A BALASUBRAMANIAM; D KANNAN

doi:10.56093/ijans.v90i7.106664

Authors

S CHITRADEVI Tamil Nadu Veterinary and Animal Sciences University, Namakkal, Tamil Nadu 637 002 India
K SUKUMAR Tamil Nadu Veterinary and Animal Sciences University, Namakkal, Tamil Nadu 637 002 India
P SURESH Tamil Nadu Veterinary and Animal Sciences University, Namakkal, Tamil Nadu 637 002 India
G A BALASUBRAMANIAM Tamil Nadu Veterinary and Animal Sciences University, Namakkal, Tamil Nadu 637 002 India
D KANNAN Tamil Nadu Veterinary and Animal Sciences University, Namakkal, Tamil Nadu 637 002 India

https://doi.org/10.56093/ijans.v90i7.106664

Keywords:

Commercial layer grower chicken, Fowl adenovirus, Gizzard erosions, PCR, Phylogenetic analysis, Sequencing

Abstract

The present study was undertaken to characterize fowl adenovirus associated with commercial layer grower chicken showed gizzard erosion. Ninety four commercial layer grower chicken flocks from Namakkal districts of Tamil Nadu had shown reduced feed intake, reduced weight gain, uneven growth and mortality of 0.3 to 7.7%. On postmortem examination of affected birds showed mild to severe gizzard erosion, blackish discoloration of gizzard contents, pale liver and no major lesions were seen in other organs. Total DNA was extracted and 897 bp fowl adenovirus specific hexon gene was amplified by PCR. Out of 94 flocks screened seven flocks were found positive of fowl adenovirus. Chicken embryo liver cell culture was prepared to isolate field fowl adenovirus from suspected flocks. Concurrent infection of chicken anaemia virus (CAV) was also screened by PCR for 419 bp VP2 gene of CAV and found that all the seven flocks which were PCR positive for FAdV also found positive for CAV. Sequencing and phylogenetic analysis of 897 bp FAdV hexon gene revealed that, it was belonged to FAdV serotypes 2 and 3 of species D.

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References

Adair B M and Fitzgerald S D. 2008. Diseases of poultry, pp. 252–266. (Eds) Y M Saif, A M Fadly, J R Glisson, L R McDougald, L K Nolan and D E Swayne. 12th edn. Iowa State University Press, Ames, IA, USA.

Bancroft J D and Stevens A. 1996. Theory and Practice of Histological Techniques. 4th edn. Churchill Livingstone, London.

Barua S and Rai A. 2003. Cultivation of fowl adenovirus 4 in chick embryo liver cell culture and purification of the virus by ultracentrifugation. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases 24: 195– 96.

Bulbule N R, Deshmukh V V, Raut S D, Meshram C D and Chawak M M. 2016. Pathogenicity and genotyping of fowl adenoviruses associated with gizzard erosion in commercial layer grower chicken in India. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases 37: 84– 91. DOI: https://doi.org/10.5958/0974-0147.2016.00015.5

Chitradevi S, Sukumar K, Suresh P, Balasubramaniam P, Kannan D and Raja A. 2018. Concurrent infections associated with fowl adenovirus infections in commercial broiler chicken. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases 39: 20–22. DOI: https://doi.org/10.5958/0974-0147.2018.00005.3

Choi K S, Kye, S J, Kim J Y, Jeon W J, Lee E K, Park K Y and Sung H W. 2012. Epidemiological investigation of outbreaks of fowl adenovirus infection in commercial chickens in Korea. Poultry Science 91: 2502–06. DOI: https://doi.org/10.3382/ps.2012-02296

Gjevre A, Kaldhusdal M and Eriksen G S. 2013. Gizzard erosion and ulceration syndrome in chickens and turkeys: a review of causal or predisposing factors. Avian Pathology 42: 297–303. DOI: https://doi.org/10.1080/03079457.2013.817665

Grafl B, Liebhart D, Gunes A, Wernsdorf P, Aigner F, Bachmeier J and Hess M. 2013. Quantity of virulent fowl adenovirus serotype 1 correlates with clinical signs, macroscopical and pathohistological lesions in gizzards following experimental induction of gizzard erosion in broilers. Veterinary Research 44: 38. DOI: https://doi.org/10.1186/1297-9716-44-38

Hess M. 2000. Detection and differentiation of avian adenoviruses: A review. Avian Pathology 29: 195–206. DOI: https://doi.org/10.1080/03079450050045440

Jadhao S J, Deepak J N, Kataria J M, Kataria R S, Tiwari A K, Somwanshi R, Sangamithra P and Verma K C.2003. Characterization of fowl adenovirus from chicken affected with infectious hydropericardium during 1994–1998 in India. Indian Journal of Experimental Biology 41: 321–27.

Lim T H, Kim B Y, Kim M S, Jang J H, Lee D H, Kwon Y K, Lee J B, Park S Y, Choi I S and Song C S. 2012. Outbreak of gizzard erosion associated with fowl adenovirus infection in Korea. Poultry Science 91: 1113–17. DOI: https://doi.org/10.3382/ps.2011-02050

Marek M, Gunes A, Schulz E and Hess M. 2010. Classification of fowl adenoviruses by use of phylogenetic analysis and high resolution melting curve analysis of the hexon L1 gene region. Journal of Virological Methods 170: 147–54. DOI: https://doi.org/10.1016/j.jviromet.2010.09.019

Mase M and Nakamura K. 2014. Phylogenetic analysis of fowl adenoviruses isolated from chickens with gizzard erosion in Japan. Journal of Veterinary Medical Sciences 76: 1535–38. DOI: https://doi.org/10.1292/jvms.14-0312

Meulemans G, Boschmans M, Van Den Berg T P and Decaesstecker M. 2001. Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathology 30: 655–60. DOI: https://doi.org/10.1080/03079450120092143

Niczyporuk J S, Salamonowicz E S and Czeka H. 2013. Analysis of adenovirus strains isolated from poultry in Poland. Bulletin of the Veterinary Institute in Pulawy 57: 305–10. DOI: https://doi.org/10.2478/bvip-2013-0053

Ono M, Okuda Y, Yazawa S, Shibata I, Sato S and Okada K. 2003. Outbreaks of adenoviral gizzard erosion in slaughtered broiler chickens in Japan. Veterinary Record 153: 775–79.

Ono M, Okuda Y,Yazawa S, Shibata I, Tanimura N, Kimura K, Haritani K, Mase M and Sato S. 2001. Epizootic outbreaks of gizzard erosion associated with adenovirus infection in chickens. Avian Diseases 45: 268–75. DOI: https://doi.org/10.2307/1593040

Ottiger H P. 2010. Development, standardization and assessment of PCR systems for purity testing of avian viral vaccines. Biologicals 38: 381–88. DOI: https://doi.org/10.1016/j.biologicals.2010.01.015

Russell W C. 2009. Adenoviruses: update on structure and function. Journal of General Virology 90: 1–20. DOI: https://doi.org/10.1099/vir.0.003087-0

Schade B, Schmitt F, Bohm B, Alex M, Fux R, Cattoli G, Terregino C, Monne I, Currie R J W and Olias P. 2013. Adenoviral gizzard erosion in broiler chickens in Germany. Avian Disease 57: 159–63. DOI: https://doi.org/10.1637/10330-082312-Case.1

Soumyalekshmi S, Ajith M K and Chandraprakash M. 2014. Isolation of fowl adenovirus in chicken embryo liver cell culture and its detection by hexon gene based PCR. Indian Journal of Scientific Research and Technology 2: 33–36.

Tanimura N, Nakamura K, Imai K, Maeda M, Gobo T, Nitta S, Ishihara T and Amano H. 1993. Necrotizing pancreatitis and gizzard erosion associated with adenovirus infection in chickens. Avian Disease 37: 606–11. DOI: https://doi.org/10.2307/1591697

Trivedi R N, Kumar R, Metwal M, Khan A S, Tiwari A, Panday G and Kumar A.2018. Epidemiological observations on some natural outbreaks of inclusion body hepatitis-hydropericardium syndrome (IBH-HPS) in domestic chicken. International Journal of Current Microbiology and Applied Sciences 7: 3012–22. DOI: https://doi.org/10.20546/ijcmas.2018.708.320

Xia J, Yao K C, Liu Y Y, Yo C J, Li S Y, Liu P, Zhao Q, Wen Y P, Wu R, Huang X B, Cao S J, Han X F and Huang Y. 2017. Isolation and molecular characterization of prevalent fowl adenovirus strains in southwestern China during 2015–2016 for the development of a control strategy. Emerging Microbes and Infections 6. DOI:10.1038/emi.2017.91. DOI: https://doi.org/10.1038/emi.2017.91