Tams 1 based PCR assay for detection of theileriosis in indigenous calves from semi-arid regions of India

KAPIL KUMAR GUPTA; MUKESH KUMAR SRIVASTAVA; VIKRANT SUDAN; DAYA SHANKER

doi:10.56093/ijans.v90i7.106671

Authors

KAPIL KUMAR GUPTA UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India
MUKESH KUMAR SRIVASTAVA UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India
VIKRANT SUDAN UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India
DAYA SHANKER UP Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281 001 India

https://doi.org/10.56093/ijans.v90i7.106671

Keywords:

BTT, Indigenous cattle calves, PCR on blood, Tams 1

Abstract

Lower parasitemia often skips the conventional microscopic and serological techniques from detecting latent, cryptic and/or chronic carrier states of bovine tropical theileriosis (BTT). Hence the molecular detection of the parasitic DNA remains a highly authenticated tool. Oligonucleotide primers (TBR F/R) were custom designed and used for PCR amplification of T. annulata. The sensitivity and specificity of PCR was compared with blood microscopy based on kappa value predictions. A total of 8 samples were found positive by blood smear examination whereas PCR detected 12 infections. Blood smear examination was kept as a gold standard for detecting actual number of confirmed positive cases, for being 100% sensitive along with 93.65% specific, respectively, in detecting calf theileriosis. The described PCR-based assay provides a valuable tool to study the epidemiology of BTT in calves and some vital data regarding epidemiology of theileriosis in calves from semi-arid parts of India has been generated. Such a record for screening of calves for theileriosis is missing from Indian context.

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