Molecular characterization of sheep and human isolates of Echinococcus granulosus from temperate region of India

A B BEIGH; M M DARZI; S BASHIR; P A DAR; B A BHAT; S NAZKI; I AHMAD

doi:10.56093/ijans.v90i8.109262

Authors

A B BEIGH Sher-e-Kashmir University of Agricultural Sciences and Technology,, Jammu and Kashmir, India
M M DARZI Sher-e-Kashmir University of Agricultural Sciences and Technology,, Jammu and Kashmir, India
S BASHIR Sher-e-Kashmir University of Agricultural Sciences and Technology,, Jammu and Kashmir, India
P A DAR Hamdard University, New Delhi, India
B A BHAT University of Otago, New Zealand
S NAZKI College of Veterinary Medicine, Chonbuk National University, South Korea
I AHMAD Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu and Kashmir 190 006 India

https://doi.org/10.56093/ijans.v90i8.109262

Keywords:

Cystic Echinococcosis, Genotyping, Human, ITS1, PCR-RFLP, Sheep

Abstract

The aim of the present study was to investigate Echinococcus granulosus genotypes in the Kashmir valley. A total of 2,100 sheep and human patients were screened for the presence of hydatidosis. Total 87 isolates were collected, 85 from sheep and 2 isolates from human beings. The rDNA-ITS1 fragment were amplified with BD1 / 4S primers. In addition, fragments of genes coding for ITS1 were sequenced. The length of amplified fragment for all isolated samples with sheep origin was 1,000 bp and with human origin was between 1,000 bp and 1,100 bp. The products on digestion with restriction enzymes Rsa 1, Alu 1, Msp 1 yielded identical fragments, 300 and 700 bp in sheep and 325 and 700 bp in humans. Intact 1000 bp fragment was observed with Taq 1. The molecular findings reveal that sheep strain (G1) is the predominant genotype in sheep and humans in Kashmir valley.

Downloads

Download data is not yet available.

References

Beigh A B, Darzi M M, Bashir S, Kashani B, Shah A and Shah S A. 2017. Pathological and histochemical studies of the effects of cystic echinococcosis in sheep. Comparative Clinical Pathology 22: 1–7.

Bhattacharya D, Bera A K, Bera B C, Maity A and Das S K. 2007. Genotypic characterisation of Indian cattle, buffalo and sheep isolates of Echinococcus granulosus. Veterinary Parasitology 143: 371–74. DOI: https://doi.org/10.1016/j.vetpar.2006.09.007

Bhattacharya D, Bera A K, Bera B C, Pan D and Das S K. 2008. Molecular appraisal of Indian animal isolates of Echinococcus granulosus. Indian Journal of Medical Research 127: 383– 87.

Bowles J and McManus D P. 1993a. Molecular variation in Echinococcus. Acta Tropica 53: 291–305. DOI: https://doi.org/10.1016/0001-706X(93)90035-A

Bowles J and Mc-Manus D P. 1993b. NADH dehydrogenase 1 gene sequence compared for species and strains of the genus Echinococcus. International Journal for Parasitology 23: 969– 72. DOI: https://doi.org/10.1016/0020-7519(93)90065-7

Bowles J and Mc-Manus D P. 1993c. Rapid discrimination of Echinococcus species and strains using a PCR-based method. Molecular Biochemistry and Parasitology 57: 231–39. DOI: https://doi.org/10.1016/0166-6851(93)90199-8

Bowles J, Blair D and Mc-Manus D P. 1995. A molecular phylogeny of the genus E. granulosus. Parasitology 110: 317– 28. DOI: https://doi.org/10.1017/S0031182000080902

Carmena D and Cardona G A. 2014. Echinococcosis in wild carnivorous species: Epidemiology, genotypic diversity, and implications for veterinary public health. Veterinary Parasitology 202: 69–94. DOI: https://doi.org/10.1016/j.vetpar.2014.03.009

Dousti M, Abdi J, Bakhtiyari S, Mohebali M, Mirhendi S H and Rokni M B. 2013. Genotyping of hydatid cyst isolated from human and domestic animals in Ilam province, Western Iran using PCR-RFLP. Iranian Journal of Parasitology 8(1): 47– 52.

Gholami S H, Sosari M, Fakhar M, Sharif M, Daryani A, Hashemi M B and Vahadi M. 2012. Molecular characterization of Echinococcus granulosus from hydatid cysts isolated from human and animals in golestan province, north of Iran. Iranian Journal of Parasitology 7: 8–16.

Hanifian H, Diba K, Tappeh K H, Mohammadzadeh H and Mahmoudlou R. 2013. Identification of Echinococcus granulosus strains in isolated hydatid Cyst specimens from animals by PCR-RFLP method in West Azerbaijan - Iran. Iranian Journal of Parasitology 8(3): 376–81.

Huttner M, Siefert L, Mackenstedt U and Romig T. 2009. A survey of Echinococcus species in wild carnivores and livestock in East Africa. International Journal for Parasitology 39: 1269– 76. DOI: https://doi.org/10.1016/j.ijpara.2009.02.015

McManus D. 2010. Echinococcosis with particular reference to Southeast Asia. Advances in Parasitology 72: 267–303. DOI: https://doi.org/10.1016/S0065-308X(10)72010-8

McManus D P, Gray D J, Zhang W and Yang Y. 2012. Diagnosis, treatment, and management of echinococcosis. British Medical Journal 344: 39–44. DOI: https://doi.org/10.1136/bmj.e3866

Pednekar R P, Gatne M L, Thompson R C A and Traub R J. 2009. Molecular and morphological characterization of Echinococcus from food producing animals in India. Veterinary Parasitology 165: 58–65. DOI: https://doi.org/10.1016/j.vetpar.2009.06.021

Sambrook J, Fritsch E F and Maniatis T. 1989. Molecular cloning: A laboratory manual (2nd edn.). Cold Spring Harbor Laboratory Press, New York.

Singh B B, Sharma J K, Ghatak S, Sharma R and Bal M S. 2012. Molecular epidemiology of Echinococcosis from food producing animals in north India. Veterinary Parasitology 186: 503–06. DOI: https://doi.org/10.1016/j.vetpar.2011.11.064

Van Herwerden L, Gasser R B and Blair D. 2000. ITS-1 ribosomal DNA sequence variants are maintained in different species and strains of Echinococcus. International Journal for Parasitology 30: 157–69. DOI: https://doi.org/10.1016/S0020-7519(00)00002-3

Arbabi M, Pirestani M, Delavari M, Hooshyar H, Abdoli A and Sarvi S. 2017. Molecular and morphological characterizations of Echinococcus granulosus from human and animal isolates in Kashan, Markazi Province, Iran. Iranian Journal of Parasitology. 12: 177–87.

Hassan H F, Fadhil M H and Fadhil Z H. 2016. Molecular characterization of Echinococcus granulosus isolated from human and domestic animals in kirkuk, Iraq. Animal Research International 13: 2544 – 47.

Carlos M and Tamara O. 2016. Molecular epidemiology of cystic Echinococcosis: Genotypic characterization in humans and different livestock. International Journal of Morphology 34: 1472–81. DOI: https://doi.org/10.4067/S0717-95022016000400048

Sharma M, Fomda B A, Mazta S, Sehgal R, Singh B B and Malla N. 2013. Genetic diversity and population genetic structure analysis of Echinococcus granulosus sensu strict complex based on Mitochondrial DNA signature. PLoS ONE 8: 1–8. DOI: https://doi.org/10.1371/journal.pone.0082904

Snabel V, Kuzmina T, Cavallero S, Amelio S D, Georgescu S O, Szenasi Z, Cielecka D, Saamatin R, Yemets A and Kucsera I. 2016. A molecular survey of Echinococcus granulosus sensu lato in central-eastern Europe. Open Life Science 11: 524–32. DOI: https://doi.org/10.1515/biol-2016-0066

Oskouei M M, Mehrabani N G, Miahipour A and Fallah E. 2016. Molecular characterization and sequence analysis of Echinococcus granulosus from sheep isolates in East Azerbaijan province, northwest of Iran. Journal of Parasitic Diseases 40: 785–90. DOI: https://doi.org/10.1007/s12639-014-0579-3

Hammada S J, Cavallerob S, Milardib G L, Gabriellib S, Ameliob S D and Al-Nasiria F S. 2018. Molecular genotyping of Echinococcus granulosus in the North of Iraq. Veterinary Parasitology 249: 82–87. DOI: https://doi.org/10.1016/j.vetpar.2017.11.010