Genetic evaluation of F2 inbred strain of Swiss albino mice by microsatellite markers

SHOBHANA KAUSHAL; PUSHPENDRA KUMAR; AMIT KUMAR; B L SAINI; MITEK TARANG; ANUJ CHAUHAN; JAI PRAKASH; BHARAT BHUSHAN

doi:10.56093/ijans.v90i9.109490

Authors

SHOBHANA KAUSHAL Division of Animal Genetics, ICAR-IVRI, Izatnagar 243 122
PUSHPENDRA KUMAR Division of Animal Genetics, ICAR-IVRI, Izatnagar 243 122
AMIT KUMAR Division of Animal Genetics, ICAR-IVRI, Izatnagar 243 122
B L SAINI Division of Animal Genetics, ICAR-IVRI, Izatnagar 243 122
MITEK TARANG Division of Animal Genetics, ICAR-IVRI, Izatnagar 243 122
ANUJ CHAUHAN Division of Animal Genetics, ICAR-IVRI, Izatnagar 243 122
JAI PRAKASH Division of Animal Genetics, ICAR-IVRI, Izatnagar 243 122
BHARAT BHUSHAN Division of Animal Genetics, ICAR-IVRI, Izatnagar 243 122

https://doi.org/10.56093/ijans.v90i9.109490

Keywords:

Genetic characterization, Inbred strain, Inbreeding coefficient, Microsatellite marker, Swiss albino mice

Abstract

Genetic characterization of F2 inbred Swiss albino mice was done using 11 microsatellites markers (D1Mit15, D2Mit51, D2Mit61, D3Mit15, D3Mit55, D5Mit18, D7Mit323, D8Mit14, D9Mit27, D10Mit180, and D11Mit167). The results indicated that genotypic frequencies at all the loci were in Hardy-Weinberg disequilibrium (P<0.001). Total number of alleles per locus ranged from 3 (D2Mit61, D3Mit15, D8Mit14, D9Mit27, D10Mit180, D11Mit167) to 4 (D1Mit15, D2Mit51, D3Mit55, D5Mit18, D7Mit323), with a mean of 3.45. The numbers of effective alleles ranged between 1.847 and 3.814. The observed heterozygosity (Ho) was maximum for D1Mit15 (0.660) and minimum for D5Mit18 (0.080), with mean of 0.269. The F_IS estimates was ranged from 0.0817 (D1Mit15) to 0.8799 (D5Mit18). The average inbreeding coefficient was 0.592, which indicates that parents were more related than expected under random mating. The range of PIC value (from 0.414 to 0.689) for various microsatellite loci was revealing that population under investigation was of high diversity maintaining a multiple allele.

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References

Basta P V, Whitmore S P, Basham K B and Whisnant C C. 2004. Microsatellite analysis in FVB/N mice. Comparative Medicine 54(5): 524–27.

Baumans V. 2004. The welfare of laboratory mice, pp. 119–52. (Ed.) Kaliste E. The Welfare of Laboratory Animals. (Animal Welfare, 2). Springer Netherlands DOI: https://doi.org/10.1007/978-1-4020-2271-5_7

Brockmann G A and Langhammer M. 1998. Characterization of small populations with microsatellite markers on mouse chromosome 11. Acta Theriologica 43(Suppl. 5): 171–77. DOI: https://doi.org/10.4098/AT.arch.98-42

Casellas J. 2011. Inbred mouse strains and genetic stability: A review. Animal 5: 1–7. DOI: https://doi.org/10.1017/S1751731110001667

Danneman P J, Suckow M A and Brayton C. 2012. The Laboratory Mouse. CRC Press. DOI: https://doi.org/10.1201/b12900

Falconer D S and Mackay T F C. 1996. Introduction to Quantitative Genetics. Longmans Green, Harlow, Essex. UK. Gulcher J. 2012. Microsatellite Markers For Linkage and Association Studies. Cold Spring Harbor Protocols 2012 (4), pdb-top068510. DOI: https://doi.org/10.1101/pdb.top068510

Lawson R, Kofron C P and Dessauer H C. 1989. Alloenzyme variation in natural populations of Nile crocodile. American Zoologist 29: 863–71. DOI: https://doi.org/10.1093/icb/29.3.863

Lukas F K and Donald M W. 2002. Inbreeding effects in wild populations. Ecology and Evolution 17(5): 230–41. DOI: https://doi.org/10.1016/S0169-5347(02)02489-8

Naghavian S, Hasani S, Ahani Azari M, Khan Ahmadi A R, Saghi D A and Mami Zade N. 2016. Estimation of Inbreeding Coefficients using Pedigree and Microsatellite markers and its effects on Economic traits of Shirvan Kordi Sheep. Iranian Journal of Applied Animal Science 6(1): 133–41.

Purohit N, Kumar M, Srivastav A K and Purohit D C P. 2015. Evaluation of genetic relationship within and between mice strains using microsatellite markers. Research in Biotechnology 6(6).

Selvaggi M, Darioa C, Peretti V, Ciotolac F, Carnicella D and Darioa M. 2010. Inbreeding depression in Leccese sheep. Journal of Small Ruminant Research 89(1): 42–46. DOI: https://doi.org/10.1016/j.smallrumres.2009.12.005

Shang H, Wei H, Yue B, Xu P and Huang H. 2009. Microsatellite analysis in two populations of Kunming mice. Laboratory Animals 43(1): 34–40. DOI: https://doi.org/10.1258/la.2008.008098

Tarang M. 2018. ‘Phenotypic and Genetic characterization of outbred and F1 inbred Swiss albino strain of mice’. MvSc. Thesis.

Voyer T E L and Hunter K W. 1999. Microsatellite DNA variants among the FVB/NJ, C58/J and I/LnJ mouse strains. Mammalian Genome 10: 542–43. DOI: https://doi.org/10.1007/s003359901040

Yeh F C, Yang R C and Boyle T. 1999. POPGENE: Microsoft Windows Based Freeware for Population Genetic Analysis. Molecular Biology and Technology Center, Unversity of Alberta. Canada.

Zhang X, Zhu Z, Huang Z, Tan P and Ma R Z. 2007. Microsatellite genotyping for four expected inbred mouse strains from KM mice. Journal of Genetics and Genomics 34(3): 214–22. DOI: https://doi.org/10.1016/S1673-8527(07)60022-8

Zuo B, Du X, Zhao J, Yang H, Wang C, Wu Y, Lu J, Wang Y and Chen Z. 2012. Analysis of microsatellite polymorphism in inbred knockout mice. PloS One 7(4): 34555. DOI: https://doi.org/10.1371/journal.pone.0034555