Studies on Detection, Prevalence and Molecular Characterization of Avian Rotavirus

Shalini Yaduvanshi; Rashmi Singh; Amitav Bhattacharya; Mohammed Ikram; Shubhankar Sircar; Yashpal Singh Malik

doi:10.56093/ijans.v92i6.109903

Authors

Shalini Yaduvanshi DUVASU Mathura
Rashmi Singh DUVASU, Mathura
Amitav Bhattacharya DUVASU, Mathura
Mohammed Ikram IVRI, Izatnagar
Shubhankar Sircar IVRI, Izatnagar
Yashpal Singh Malik GADVASU, Ludhiana

https://doi.org/10.56093/ijans.v92i6.109903

Keywords:

Avian rotavirus, RVD, NSP5, Phylogenetic analysis

Abstract

Rotaviruses are the foremost cause of acute gastroenteritis. Outbreaks of rotavirus infection can lead to significant economic losses in livestock and poultry industry. Group A and D rotavirus are the predominant enteric viruses groups in birds. Limited prevalence studies are available to date in India. Poultry samples consisting of 20 fecal samples and 80 intestinal content samples were screened by VP6 gene based diagnostic PCR for AvRVA and AvRVD. All samples were negative for group A rotavirus. Five fecal samples and 22 intestinal samples were positive for AvRVD-VP6 gene giving an amplicon of 185 bp giving a prevalence of 27%. Positive samples screened for VP4, VP7, NSP4 and NSP5 genes of AvRVD were negative for VP4, VP7 and NSP4 but six samples were positive for NSP5 gene giving an amplicon of 652 bp size. Cloning and sequencing showed 99.4 to 100% sequence similarity among all six isolates at nucleotide level. The percent nucleotide similarity with chicken RVD isolates was in the range 78.2-85.1%. All isolates showed highest similarity of 85% with chicken isolate from South Korea followed by Germany (81.5%) and Australia (78.2%). The phylogenetic analysis exhibited all six isolates clustered together in a separate clade with isolates from Australia, Germany and South Korea.

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Author Biographies

Shalini Yaduvanshi, DUVASU Mathura

PhD Scholar
College of Biotechnology
Rashmi Singh, DUVASU, Mathura

Professor and Head
Department of Veterinary Microbiology
Amitav Bhattacharya, DUVASU, Mathura

Assistant Professor, Poultry Science
Mohammed Ikram, IVRI, Izatnagar

Research Associate
Division of Biological Standardsiation
Shubhankar Sircar, IVRI, Izatnagar

Senior Research Fellow
Division of Biological Standardisation
Yashpal Singh Malik, GADVASU, Ludhiana

Dean, College of Animal Biotechnology

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