Genetic polymorphism in HSPB6 gene and their association with heat tolerance in Sahiwal cattle
Abstract views: 68 / PDF downloads: 79
Keywords:Haplotype, Heat shock protein B6, Heat stress, Sahiwal cattle, SNP
Heat shock proteins (HSPs) are known to modulate cellular response during summer stress in dairy cattle. Among different classes of HSPs, heat shock protein 20 (HSPB6) is a member of the small HSP family protein, the role of which has not been fully characterized in the context of heat stress in cattle. This study identified single nucleotide polymorphisms (SNPs) in the HSPB6 gene in Sahiwal cattle and their associations with heat tolerance traits (RR, RT and HTC). Three SNPs (SNP 1-3) were reported, which included two transitions, viz. SNP1-g.436G>A (Intron 1) and SNP2-g.2152A>G (3′-UTR) and one transversion, viz. SNP3-g.2417A>T (3′-UTR). The association analysis revealed that SNPs loci, viz. SNP1-g.436G>A and SNP2-g.2152A>G were significantly associated with heat tolerance traits. The GG genotype of SNP2-g.2152A>G was significantly associated with heat tolerance traits in Sahiwal cattle. The association analysis of four available haplotypes, viz. Hap1 (GGA), Hap2 (AAA), Hap3 (GAA), and Hap4 (AAT) of HSPB6 gene with heat tolerance traits did not differ significantly with any haplotype in Sahiwal cattle. This study provides the first association analyses between the SNPs of HSPB6 gene and heat tolerance traits in Sahiwal cattle, which could be used as effective SNP markers in genetic selection for heat tolerance in cattle breeding programs.
Arjomandfar M, Zamiri M J, Rowghani E, Khorvash M and Ghorbani G H. 2010. Effects of water desalination on milk production and several blood constituents of Holstein cattle in a hot arid climate. Iranian Journal of Veterinary Research 11: 233–38.
Barrett J C, Fry B, Maller J and Daly M J. 2005. Haploview: Analysis and visualization of LD and haplotype maps. Bioinformatics 21: 263–65. DOI: https://doi.org/10.1093/bioinformatics/bth457
Beatty D T, Barnes A, Taylor E, Pethick D, Mccarthy M and Maloney S K. 2006. Physiological responses of Bos taurus and Bos indicus cattle to prolonged, continuous heat and humidity. Journal of Animal Science 84: 972–85. DOI: https://doi.org/10.2527/2006.844972x
Benezra M V. 1954. A new index for measures the adaptability of cattle to tropical condition. Proceedings. Journal of Animal Science 13: 1015.
Bhanuprakash V, Singh U, Sengar G, Sajjanar B, Bhusan B, Raja T V, Alex R, Kumar S, Singh R, Ashish alyethodi R R, Kumar S and Deb R. 2016. Differential effect of thermal stress on HSP70 expression, nitric oxide production and cell proliferation among native and crossbred dairy cattle. Journal of Thermal Biology 59: 18–25. DOI: https://doi.org/10.1016/j.jtherbio.2016.04.012
Boonstra R. 2004. Coping with changing northern environments: the role of the stress axis in birds and mammals. Integrative and Comparative Biology 44(2): 95–108. DOI: https://doi.org/10.1093/icb/44.2.95
Charoensook R, Gatphayak K, Sharifi A R, Chaisongkram C, Brenig B and Knorr C. 2012. Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle. Tropical Animal Health and Production 44: 921–28. DOI: https://doi.org/10.1007/s11250-011-9989-8
Cross B E and Dea H M. 2007. Expression of small heat shock- related protein 20 (HSP20) in rat myometrium is markedly decreased during late pregnancy and labour. Reproduction 133(4): 807–17. DOI: https://doi.org/10.1530/REP-06-0291
Deb R, Sajjanar B, Singh U, Kumar S, Sengar G, Alex R, Das A K, Tyagi S, Raja T V, Alyethodi R R, Singh R, Bhanuprakash V and Prakash B. 2016. Cytosine deletion at AP2 box region of HSP70 promoter and its influence on semen quality traits in crossbred bulls. Journal of Genetics 95 (4): 1035–38. DOI: https://doi.org/10.1007/s12041-016-0727-x
Dreiza C M, Komalavilas P, Furnish E J, Flynn C R, Sheller M R, Smoke C C, Lopes L B and Brophy C M. 2010. The small heat shock protein, HSPB6, in muscle function and disease. Cell Stress Chaperon 15(1): 1–11. DOI: https://doi.org/10.1007/s12192-009-0127-8
Ellis R J. 2006. Protein Misassembly: Macromolecular Crowding and Molecular Chaperones. Harbor Laboratory Press, Cold Spring Harbor, New York.
Hansen P J. 2004. Physiological and cellular adaptations of zebu cattle to thermal stress. Animal Reproduction Science 82-83: 349–60. DOI: https://doi.org/10.1016/j.anireprosci.2004.04.011
Hoffmann I. 2010. Climate change and the characterization, breeding and conservation of animal genetic resources. Animal Genetics 41(1): 32–46. DOI: https://doi.org/10.1111/j.1365-2052.2010.02043.x
Kato K, Goto S, Inaguma Y, Hasegawa K, Morishita R and Asano T. 1994. Purification and characterization of a 20-kDa protein that is highly homologous to alpha B crystallin. Journal of Biological Chemistry 269(21): 15–30. DOI: https://doi.org/10.1016/S0021-9258(17)36606-1
Komalavilas P and Penn R B. 2008. The small heat shock-related protein, HSP20, is a cAMP-dependent protein kinase substrate that is involved in airway smooth muscle relaxation. American Journal of Physiology 294(1): 69–78. DOI: https://doi.org/10.1152/ajplung.00235.2007
Kregel K C. 2002. Heat shock proteins: Modifying factors in physiological stress responses and acquired thermotolerance. Journal of Applied Physiology 92: 2177–86. DOI: https://doi.org/10.1152/japplphysiol.01267.2001
Kumar R, Gupta I D, Verma A, Singh S, Kumari R and Verma N. 2021. Genetic polymorphism in HSPB6 gene and their association with heat tolerance traits in Indian Karan Fries (Bos taurus × Bos indicus) cattle. Animal Biotechnology. DOI: https://doi.org/10.1080/10495398.2021.1899939
Kumar R, Gupta I D, Verma A, Kumari R and Verma N. 2017. Molecular characterization and SNP identification in HSPB6 gene in Karan Fries (Bos taurus × Bos indicus) cattle. Tropical Animal Health and Production 49(3). DOI: https://doi.org/10.1007/s11250-017-1235-6
Kumar R, Gupta I D, Verma A, Verma N and Vineeth M R. 2015. Genetic polymorphisms within exon 3 of heat shock protein 90AA1 gene and its association with heat tolerance traits in Sahiwal cows. Veterinary World 8(7): 932–36. DOI: https://doi.org/10.14202/vetworld.2015.932-936
Kumar R, Gupta I D, Verma A, Verma N, Das R and Vineeth M R. 2015. Molecular characterization and polymorphism detection in HSPB6 gene in Sahiwal cattle. Indian Journal of Animal Research 49(5): 595–98. DOI: https://doi.org/10.18805/ijar.5568
Lacetera N, Bernabucci U, Scalia D, Basirico L, Morera P and Nardone A. 2006. Heat stress elicits different responses in peripheral blood mononuclear cells from Brown Swiss and Holstein cattle. Journal of Dairy Science 89: 4606–12. DOI: https://doi.org/10.3168/jds.S0022-0302(06)72510-3
Li Q L, Ju Z H, Huang J M, Li J B, Li R L, Hou M H, Wang C F and Zhong J F. 2011. Two novel SNPs in HSF1 gene are associated with thermal tolerance traits in Chinese Holstein cattle. DNA Cell Biology 30: 247–54. DOI: https://doi.org/10.1089/dna.2010.1133
Li Z Q, Zhang Z, He Z D, Tang W, Li T, Zeng Z, He L and Shi Y. 2009. A partition-ligation-combination-subdivision EM algorithm for haplotype inference with multiallelic markers. Cell Research 19: 519–23. DOI: https://doi.org/10.1038/cr.2009.33
Morimoto R I and Santoro M G. 1998. Stress-inducible responses and heat shock proteins: new pharmacologic targets for cytoprotection. Nature Biotechnology 16: 833–38. DOI: https://doi.org/10.1038/nbt0998-833
Mymrikov E V, Seit-Nebi A S and Gusev N B. 2011. Large potentials of small heat shock proteins. Physiological Review 91: 1123–59. DOI: https://doi.org/10.1152/physrev.00023.2010
Pipkin W and Johnson J A. 2003. Localization, macromolecular associations, and function of the small heat shock-related protein HSP20 in rat heart. Circulation 107(3): 469–76. DOI: https://doi.org/10.1161/01.CIR.0000044386.27444.5A
Rong Y U, Zeng M, Guan X, Li J B, Li R L, Hou M H, Wang C F and Zhong J F. 2019. Association of HSF1 genetic variation with heat tolerance in chinese cattle. Animals 9: 1027. DOI: https://doi.org/10.3390/ani9121027
Sailo L, Gupta I D, Verma N, Das R, Chaudhari M V and Singh S. 2016. Polymorphisms in Hsp90ab1 gene and their association with heat tolerance in Sahiwal and Karan Fries cattle. Indian Journal of Animal Research 50(6): 856–61. DOI: https://doi.org/10.18805/ijar.v0iOF.6662
Sajjanar B, Deb R, Singh U, Kumar S, Brahmane M, Nirmale A, Bal S K and Minhas P S. 2015. Identification of SNP in HSP90AB1 and its association with the relative thermotolerance and milk production traits in Indian dairy cattle. Animal Biotechnology 26(1): 45–50. DOI: https://doi.org/10.1080/10495398.2014.882846
Sambrook J and Russell D W. 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Seath D M and Miller G D. 1946. The relative importance of high temperature and pulse rate of dairy cattle. International Journal of Biometeorology 27: 47–62.
Shi Y Y and He L. 2005. SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction, and genetic association at polymorphism loci. Cell Research15: 97–98. DOI: https://doi.org/10.1038/sj.cr.7290272
Srikandakumar A and Johnson E H. 2004. Effect of heat stress on milk production, rectal temperature, respiratory rate and blood chemistry in Holstein, Jersey and Australian Milking Zebu cattle. Tropical Animal Health Production 36: 685–92. DOI: https://doi.org/10.1023/B:TROP.0000042868.76914.a9
Tessier D J and Komalavilas P. 2004. Transduction of peptide analogs of the small heat shock-related protein HSP20 inhibits intimal hyperplasia. Journal of Vascular Surgery 40(1): 106–14. DOI: https://doi.org/10.1016/j.jvs.2004.03.028
Thom E C. 1959. The discomfort index. Weatherwise 12: 57–59. Upadhyay R C, Sirohi S, Ashutosh, Singh S V, Kumar A and Gupta S K. 2009. Impact of climate change on milk production in India. (Ed.) Aggarwal P K. Global Climate Change and Indian Agriculture. Published by ICAR, New Delhi.
Verma N, Gupta I D, Verma A, Kumar R, Das R and Vineeth M R. 2015. Novel SNPs in HSPB8 gene and their association with heat tolerance traits in Sahiwal indigenous cattle. Tropical Animal Health and Production 48(1): 175–80 DOI: https://doi.org/10.1007/s11250-015-0938-9
Wang Y J, Huang J, Xia P, He J, Wang C, Ju Z, Li J, Li R, Zhong J and Li Q. 2013. Genetic variations of HSBP1 gene and its effect on thermal performance traits in Chinese Holstein cattle. Molecular Biology Report 40: 3877–82. DOI: https://doi.org/10.1007/s11033-012-1977-1
West J W. 2003. Effects of heat-stress on production in dairy cattle. Journal of Dairy Science 86: 2131–44. DOI: https://doi.org/10.3168/jds.S0022-0302(03)73803-X
Yeh F C, Yang R and Bozle T. 1999. POPGENE version 1.31, MS Windows-based freeware for population genetic analysis. University of Alberta and Center for International Forestry Research.
How to Cite
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.