Effects of vitamin B1, vitamin E and selenium on pregnancy and blood metabolites profile during non-breeding season and early prediction of pregnancy by thermographic monitoring in Merino ewes

MURAT ONUR YAZLIK; KEMAL TUNA OLGAC; HATICE ESRA COLAKOGLU; UFUK KAYA; MERVE MENEKSE YILDIRIM; BULENT BAS

doi:10.56093/ijans.v90i10.111306

Authors

MURAT ONUR YAZLIK Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey
KEMAL TUNA OLGAC Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey
HATICE ESRA COLAKOGLU Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey
UFUK KAYA Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey
MERVE MENEKSE YILDIRIM Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey
BULENT BAS Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey

https://doi.org/10.56093/ijans.v90i10.111306

Keywords:

Ewe, Synchronization, Thermography

Abstract

The objective of this study was to evaluate the effects of injectable selenium, vitamin E and vitamin B1 compounds on serum metabolic profile and pregnancy rates (PR) during the non-breeding season in Merino ewes that were synchronized with progesterone and equine chorionic gonadotrophin (eCG). Thermographic monitoring of ewes on the last day of synchronization was successful in the early selection of ewes to be used in artificial insemination and its relationship with pregnancy outcomes. Cyclic and non-pregnant ewes (n=75) were randomly assigned to treatment (n=30) and control (n=45) groups. All animals were synchronized using PGF2α at day 0, progesterone impregnated intravaginal sponges for 14 days, eCG 48 h before sponge withdrawal and artificial insemination were carried out 43–45 h after sponge removal. Animals of treatment group (n=30) were administered (intramuscularly) with 1 mg sodium selenite, 60 mg vitamin E and 40 mg vitamin B1 on two occasions, at the beginning of sponge insertion and following sponge removal. Blood samples were collected at sponge insertion and removal. Perianal and perivulvar areas were considered to evaluate the thermal pattern at day 0 and 14 using a thermo camera. Overall, 90.6% of ewes exhibited estrus in response to the synchronization protocol. Ewes of treatment group showed greater PR as compared to that of control group (70.3% vs 56%). Perivulvar temperature was influenced by time only. At the time of sponge removal, perivulvar temperatures were significantly lower in pregnant ewes than in non-pregnant animals. In conclusion, injectable selenium, vitamin E and vitamin B1 compounds increased PR after synchronization protocol and thermographic evaluation that was performed on the last day of synchronization could be used for very early prediction of pregnancy.

Downloads

Download data is not yet available.

References

Abdollahi E, Kohram H, Shahir M H and Nemati M H. 2015. Effects of a sustained-release multi-trace element ruminal bolus on sex ratio, reproductive traits and lambs growth in synchronized Afshari ewes. Iranian Journal of Veterinary Science and Technology 7(1): 1–11.

Awawdeh M S, Talafha A Q and Obeidat B S. 2015. Postpartum injection with vitamin E and selenium failed to improve the performance of Awass iewes and their lambs. Canadian Journal of Animal Science 95(1): 111–15. DOI: https://doi.org/10.4141/cjas-2014-099

Benoit M, Sabatier R, Lasseur J, Creighton P and Dumont B. 2019. Optimising economic and environmental performances of sheep-meat farms does not fully fit with the meat industry demands. Agronomy for Sustainable Development 39: 40.1– 11. DOI: https://doi.org/10.1007/s13593-019-0588-9

Brewer M S. 2011. Natural antioxidants: sources. compounds. mechanisms of action and potential applications. Comprehensive Reviews in Food Science and Food Safety 10(4): 21–27. DOI: https://doi.org/10.1111/j.1541-4337.2011.00156.x

Gabryszuk M and Klewiec J. 2002 Effect of injecting 2-and 3- year-old ewes with selenium and selenium–vitamin E on reproduction and rearing of lambs. Small Ruminant Research 43(2): 127–32. DOI: https://doi.org/10.1016/S0921-4488(02)00005-6

Kose M, Kýrbas M, Dursun S and Bayrýl T. 2013. The effect of injections of -carotene or vitamin E + Selenium on fertility in ewes in anestrus season. Yüzüncüyýl Üniversitesi Veteriner Fakültesi Dergisi 24(2): 83–86.

Koyuncu M and Yerlikaya H. 2007. Effect of selenium-vitamin E injections of ewes on reproduction and growth of their lambs. South African Journal of Animal Science 37(4): 233– 36. DOI: https://doi.org/10.4314/sajas.v37i4.4095

Kuru M, Sogukpinar O, Makav M and Cetin N. 2017. Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during non-breeding season. Medycyna Weterynaryjna 73(8): 479–82. DOI: https://doi.org/10.21521/mw.5758

Martinez M F, McLeod B, Tattersfield G, Smaill B, Quirke L D and Juengel J L. 2015. Successful induction of oestrus. Ovulation and pregnancy in adult ewes and ewe lambs out of the breeding season using a GnRH + progesterone oestrus synchronisation protocol. Animal Reproduction Science 155: 28–35. DOI: https://doi.org/10.1016/j.anireprosci.2015.01.010

Martinez-Ros P and Gonzalez-Bulnes A. 2019. Efficiency of CIDR-based protocols including GnRH instead of eCG for estrus synchronization in Sheep. Animals 9(4): 1–11. DOI: https://doi.org/10.3390/ani9040146

Mudd A J and Mackie I L. 1973. The influence of vitamin E and selenium on ewe prolificacy. Veterinary Record 93(7): 197– 99. DOI: https://doi.org/10.1136/vr.93.7.197

Murthy V K and Shipp J C. 1981. Regulation of hepatic triglyceride synthesis in diabetic rats. Journal of Clinical Investigation 67(4): 923–30. DOI: https://doi.org/10.1172/JCI110141

Simoes V G, Lyazrhi F, Picard-Hagen N, Gayrard V, Martineau G P and Waret Szkuta A. 2014. Variations in the vulvar temperature of sows during proestrus and estrus as determined by infrared thermography and its relation to ovulation. Theriogenology 82(8): 1080–85. DOI: https://doi.org/10.1016/j.theriogenology.2014.07.017

Singh I D, Sinha S N, Singh B and Verma S B. 1983. Effect of some oestrus inducing drugs on total serum cholesterol level in cattle. Indian Journal of Animal Reproduction 22(2): 157– 60.

Sonmez M, Bozkurt T, Türk G, Gur S, Kýzýl M and Yüce A. 2009. The effect of vitamin E treatment during preovulatory period on reproductive performance of goats following estrous synchronization using intravaginal sponges. Animal Reproduction Science 114(1–3): 183–92. DOI: https://doi.org/10.1016/j.anireprosci.2008.09.007

Stelletta C, Tekin K, Tirpan M B, Alemdar H, Cil B, Stelletta F O, Olgac K T, Inanc M E and Daskin A. 2017. Vulvar thermal pattern following synchronization of estrus is linked to fertility after timed artificial insemination in goat. Theriogenology 103: 137–42. DOI: https://doi.org/10.1016/j.theriogenology.2017.07.038

Sykes D J, Couvillion J S, Cromiak A, Bowers S, Schenck E, Crenshaw M and Ryan P L. 2012. The use of digita linfrared thermal imaging to detect estrus in gilts. Theriogenology 78: 147–52. DOI: https://doi.org/10.1016/j.theriogenology.2012.01.030

Talukder S, Kerrisk K L, Ingenhoff L, Thomson P C, Garcia S C and Celi P. 2014. Infrared technology for estrus detection and as a predictor of time of ovulation in dairy cows in a pasturebased system. Theriogenology 81: 925–35. DOI: https://doi.org/10.1016/j.theriogenology.2014.01.009

Wani J M, Sharma U, Beigh S A, Haq S U, Khan A A S, Pandey A K and Haq Z. 2018. Evaluation of biochemical profile of estrus induced ewes during nonbreeding season. Journal of Entomology and Zoology Studies 6(1): 796–99.