Immunohistochemical localization of Kisspeptin and its receptor in the placentome of buffalo (Bubalus bubalis)

G K MISHRA; M K PATRA; M KARIKALAN; S K SINGH; H KUMAR; N KRISHNASWAMY

doi:10.56093/ijans.v92i7.111606

Authors

G K MISHRA Assistant Professor, Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, Anjora, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh 491 001, India
M K PATRA Scientist, ICAR- Indian Veterinary Research Institute, Izatnagar, U.P., 243 122
M KARIKALAN Scientist, Centre for Wildlife Conservation, Management and Disease Surveillance, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 234 122, India
S K SINGH Principal Scientist, Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 234 122, India
H KUMAR Principal Scientist and Head, Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 234 122, India
N KRISHNASWAMY Principal Scientist, ICAR-Indian Veterinary Research Institute, Bengaluru campus, Hebbal, Bengaluru, India

https://doi.org/10.56093/ijans.v92i7.111606

Keywords:

Buffalo, Immunolocalization, Kisspeptin, Kiss1r, Placentome

Abstract

Evidence for extra-hypothalamic expression of kisspeptinergic system is emerging. The present study documents the imuunolocalization of the kisspeptin and its receptor in the placentome of buffalo. Gravid uterus was collected from the abattoir and gestational age was estimated by crown-rump length of the fetus. A section of placentome containing the caruncle and cotyledon was used for immunohistochemistry. The expression of Kp and its receptor was evident in the feto-maternal junctions with prominent localization in the mononucleate and binucleate cells. In conclusion, the present study supports possible role of placental kisspeptin during pregnancy in the ruminants

Downloads

Download data is not yet available.

References

Caraty A, Franceschini I and Hoffman G E. 2011. Kisspeptin and the preovulatory gonadotrophin-releasing hormone/

luteinising hormone surge in the ewe: Basic aspects and potential applications in the control of ovulation. Journal of

Neuroendocrinology 22: 710–15.

Horikoshi Y, Matsumoto H, Takatsu Y, Ohtaki T, Kitada C, Usuki S and Fujino M. 2003. Dramatic elevation of plasma metastin concentrations in human pregnancy: Metastin as a novel placenta-derived hormone in humans. Journal of Clinical Endocrinology and Metabolism 88: 914–19.

Hu K L, Chang H M, Zhao H C, Yu Y, Li R and Qiao J. 2019. Potential roles for the kisspeptin/kisspeptin receptor system

in implantation and placentation. Human Reproduction Update 25(3): 326–43.

Kotani M, Detheux M, Vandenbogaerde A, Communi D, Vanderwinden JM, Le Poul E, Brezillon S, Tyldesley R, Suarez-Huerta N, Vandeput F, Blanpain C, Schiffmann S N, Vassart G and Parmentier M. 2001. The metastasis suppressor

gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein coupled receptor GPR54. The Journal of

Biological Chemistry 276: 34631–36.

Mishra G K, Patra M K, Singh L K, Sheikh P A, Upmanyu V, Chakravarti S, Karikalan M, Sonwane A, Singh S K, Das G K,

Kumar H and Narayanan K. 2019b. Expression of kisspeptin and its receptor in the hypothalamus of cyclic and acyclic

buffalo (Bubalus bubalis). Theriogenology 139: 167–77.

Mishra G K, Patra M K, Singh L K, Upmanyu V, Chakravarti S, Karikalan M, Singh S K, Das G K, Kumar H and Narayanan

K. 2019a. Kiss1 and its receptor: Molecular characterization and immunolocalization in the hypothalamus and corpus

luteum of the buffalo. Animal Biotechnology 30(4): 342–51.

Mishra G K, Patra M K, Singh L K, Upmanyu V, Chakravarti S, Karikalan M, Bag S, Singh S K, Das G K, Kumar H and

Narayanan K. 2019c. Expression and functional role of kisspeptin and its receptor in the cyclic corpus luteum of

buffalo (Bubalus bubalis). Theriogenology 130: 71–8.

Mondal M, Baruah K K and Prakash B S. 2015.Determination of plasma kisspeptin concentrations during eproductive

cycle and different phases of pregnancy in crossbred cows using bovine specific enzyme immunoassay. General and

Comparative Endocrinology 224: 168–75.

Niswender G D, Juengel J L, Silva P J, Rollyson M K and McIntush E W. 2000. Mechanisms controlling the function

and life span of the corpus luteum. Physiological Reviews 80: 1–29.

Ohtaki T, Shintani Y, Honda S, Matsumoto H, Hori A, Kanehashi K, Terao Y, Kumano S, Takatsu Y, Masuda Y, Ishibashi Y, Watanabe T, Asada M, Yamada T, Suenaga M, Kitada C, Usuki S, Kurokawa T, Onda H, Nishimura O and Fujino M.

Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor. Nature 411: 613–17.

Rizzo A, Ceci E, Guaricci A C and Sciorsci R L. 2019. Kisspeptin in the early post–partum of the dairy cow. Reproduction in Domestic Animals 54(2): 195–98. DOI: https://doi.org/10.1111/rda.13325

Terao Y, Kumano S, Takatsu Y, Hattori M, Nishimura A, Ohtaki T and Shintani Y. 2004. Expression of KiSS-1, a etastasis

suppressor gene, in trophoblast giant cells of the rat placenta. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression 1678: 102–10.