Effect of buffalo PDC-109 on caudal epididymal spermatozoa of bubaline species

S S RAMTEKE; J S RAJORIYA; A M SHENDE; S K GHOSH; J K PRASAD; P PERUMAL; M SINGH

doi:10.56093/ijans.v90i12.113166

Authors

S S RAMTEKE ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
J S RAJORIYA College of Veterinary and Animal Husbandry, Rewa, Madhya Pradesh
A M SHENDE Nagpur Veterinary College, Nagpur, Maharashtra
S K GHOSH ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh
J K PRASAD ICAR-Central Island Agricultural Research Institute, Port Blair, Andaman and Nicobar Islands
P PERUMAL ICAR-ICAR Research Complex for NEH Region, Nagaland Centre, Medziphema, Nagaland
M SINGH ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v90i12.113166

Keywords:

Buffalo, Caudal epididymal spermatozoa, In vitro capacitation, In vitro fertilization, PDC-109

Abstract

A study was conducted to assess the effect of buffalo seminal PDC-109 on its cauda epididymal spermatozoa. PDC-109 protein was purified by applying two-step chromatography procedures and included into epididymal spermatozoa. Epididymal ejaculates were splited into four groups as Gr 1: Control (without PDC), Gr 2: 20 Î¼g/mL PDC-109, Gr 3: 40 Î¼g/mL PDC-109 and Gr 4: 80 Î¼g/mL PDC-109. Semen quality parameters (SQPs) and in vitro fertility assay were evaluated. Significant improvement in post-thaw SQPs was observed in Gr 2 than in Gr 3, however, Gr 1 had significantly higher value than other groups. It is concluded that PDC-109 has dose dependent effect as increased dose causes detrimental effect in buffalo cauda epididymal sperm.

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