Efficacy of evaluation of mitochondrial membrane potential in equine spermatozoa using JC-1


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Authors

  • T R TALLURI ICAR-National Research Centre on Equines, Bikaner, Rajasthan 334 001 India
  • ANURADHA BHARDWAJ ICAR-NRC on Equines, Hisar, Haryana
  • S C MEHTA ICAR-National Research Centre on Equines, Bikaner, Rajasthan 334 001 India
  • YASH PAL ICAR-National Research Centre on Equines, Bikaner, Rajasthan 334 001 India

https://doi.org/10.56093/ijans.v90i12.113169

Keywords:

Equine, Fluorescence, JC-1, Mitochondrial membrane potential, Motility, Sperm

Abstract

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References

Allesandra G, Lacalandra G M, Filannino A, Flavia P, Nicassio M, Dell’Aquila M E and Minervini F. 2010. Assessment of viability, chromatin structure stability, mitochondrial function and motility of stallion fresh sperm by using objective methodologies. Journal of Cell and Animal Biology 4: 34– 41.

Cossarizza A, Ceccarelli D and Masini A. 1996. Functional heterogeneity of an isolated mitochondrial population revealed by cytofluorometric analysis at the single organelle level. Experimental Cell Research 222: 84–94. DOI: https://doi.org/10.1006/excr.1996.0011

Gillan L, Evans G and Maxwell W M C. 2005. Flow cytometric evaluation of sperm parameters in relation to fertility potential period. Theriogenology 63: 445–57. DOI: https://doi.org/10.1016/j.theriogenology.2004.09.024

Hafez B and Hafez E S E. 2000. Reproduction in Farm Animals. 7th ed. New York. Lippincott Williams and Wilkins, USA. Pp. 274–281. DOI: https://doi.org/10.1002/9781119265306

Jasko D J, Little T V and Foote R H. 1991. Determination of stallion semen quality and its relationship with fertility. Journal of Reproduction and Fertility 44(Suppl): 649–50.

Martinez-Pastor F, Johannisson A, Gil J, Kaabi M, Anel L, Paz P and Rodriguez-Martinez H. 2004. Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate a frozen-thawed ram semen. Animal Reproduction Science 84: 121–33. DOI: https://doi.org/10.1016/j.anireprosci.2003.12.006

Moce E and Graham J K. 2008. In vitro evaluation of sperm quality. Animal Reproduction Science 105: 104–18. DOI: https://doi.org/10.1016/j.anireprosci.2007.11.016

Reers M, Smith T W and Chen L B. 1991. J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential. Biochemistry 7: 4480–86. DOI: https://doi.org/10.1021/bi00232a015

Smiley S T, Reers M, Mottola-Hartshorn C, Lin M, Chen A, Smith T W, Steele G D and Chen L B. 1991. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a Jaggregate-forming lipophilic cation JC-1. Proceedings of National Academy of Sciences (USA) 88: 3671–75. DOI: https://doi.org/10.1073/pnas.88.9.3671

Vamer D D, Vaugn S D and Johnson L. 1991. Use of a computerized system for the evaluation of equine spermatozoa1 motility. American Journal of Veterinary Research 52: 224–30.

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Submitted

2021-07-27

Published

2021-07-28

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Section

Short-Communication

How to Cite

TALLURI, T. R., BHARDWAJ, A., MEHTA, S. C., & PAL, Y. (2021). Efficacy of evaluation of mitochondrial membrane potential in equine spermatozoa using JC-1. The Indian Journal of Animal Sciences, 90(12), 1599-1601. https://doi.org/10.56093/ijans.v90i12.113169
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