Dynamics of mitochondrial membrane potential and DNA damage during cryopreservation of cattle and buffalo bull spermatozoa

KAMARAJ ELANGO; ARUMUGAM KUMARESAN; MANOKARAN ASHOKAN; THIRUMALAISAMY KARUTHADURAI; PRADEEP NAG; MULINTI BHASKAR; BAKTHAVATHSALAM ARUN PRASAD; SAKTHIVEL JEYAKUMAR; AYYASAMY MANIMARAN; VINOD BHAT; KEREKOPPA RAMESHA

doi:10.56093/ijans.v91i1.113218

Authors

KAMARAJ ELANGO Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
ARUMUGAM KUMARESAN Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
MANOKARAN ASHOKAN Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
THIRUMALAISAMY KARUTHADURAI Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
PRADEEP NAG Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
MULINTI BHASKAR Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
BAKTHAVATHSALAM ARUN PRASAD Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
SAKTHIVEL JEYAKUMAR Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
AYYASAMY MANIMARAN Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
VINOD BHAT Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru
KEREKOPPA RAMESHA Southern Regional Station-National Dairy Research Institute (SRS-NDRI), Adugodi, Bengaluru

https://doi.org/10.56093/ijans.v91i1.113218

Keywords:

Cryopreservation, DNA damage, Equilibration, Mitochondrial membrane potential

Abstract

Understanding the changes in the spermatozoa during cryopreservation is indispensable for tailoring and increasing the efficiency of cryopreservation process success. However, the dynamics of damage to sperm organelles during different stages of cryopreservation is underexplored. This study assessed the mitochondrial membrane potential (MMP) and DNA damage during different stages of cryopreservation, viz. immediately after ejaculation, after equilibration and after freezing and thawing in cattle and buffalo spermatozoa using flow cytometry. Proportion of spermatozoa with high MMP decreased significantly after equilibration (from 66.06±4.59 to 42.58±6.30 in Holstein bulls and from 60.32±5.51 to 39.98±7.58 in buffalo bulls). Sperm DNA integrity [DNA fragmentation index (DFI %)] in Holstein Friesian (HF) bulls did not differ significantly between fresh and equilibrated samples but a significantly higher % DFI was observed in frozen-thawed semen samples as compared to both fresh and equilibrated samples. In contrast, % DFI in buffalo spermatozoa did not differ among the three stages of cryopreservation. It was concluded that mitochondrial damages occur during equilibration while chromatin damages occur during freezing and thawing of cattle bull spermatozoa; whereas buffalo bull spermatozoa were lesser susceptible to DNA damage during cryopreservation as compared to cattle spermatozoa.

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