Association of milk production traits with genetic variants in exon 5 and intron 3 of bovine growth hormone (bGH) gene in Sahiwal cattle

SHWETA SACHAN; I D GUPTA; ARCHANA VERMA; ANSHUMAN KUMAR; M R VINEETH

doi:10.56093/ijans.v91i1.113271

Authors

SHWETA SACHAN Animal Genetics and Breeding Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, Uttar Pradesh, India
I D GUPTA ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
ARCHANA VERMA ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
ANSHUMAN KUMAR ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
M R VINEETH ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v91i1.113271

Keywords:

Bovine growth hormone, GH-AluI, GH-MspI, Production traits, Sahiwal cows

Abstract

The main objective of this study was to determine the association of production traits with genetic variants in exon 5 and intron 3 of bovine growth hormone (bGH) gene in Sahiwal cattle. The analyses were based on the detection of single nucleotide polymorphisms (SNPs) in GH-AluI (exon 5) and GH-MspI (intron 3) using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The frequency of AluI (L/V) and MspI (T/C) alleles were 86.20/13.70 and 89.80/9.50, respectively. The distribution of the frequency of GH genotypes for LL, LV, and VV were 73.4, 25.5, and 1.0 and for TT, TC, and CC genotypes were 80.9, 17.7, and 13.0, respectively. Season of calving had none but period of calving had significant effect on the studied production traits. The phenotypic data was adjusted for non-genetic factors and regression analysis was done. There was detectable significant effect of the GH-AluI and GH-MspI on the analyzed production traits. The cows with LL genotype had significantly higher milk yield traits than the LV genotype. Similarly, the productive performance of the studied cows with TT genotype was also significantly higher than TC and CC genotypes. Results revealed that the Sahiwal cows with LL and TT genotypes of the bGH locus can be considered to be a favourable genotype for production traits, although these findings need to be confirmed by further research before SNPs can be used in a marker assisted selection program by the animal breeder.

Downloads

Download data is not yet available.

References

Amiri S, Jemmali B, Ferchichi M A, Jeljeli H, Boulbaba R and Gara A B. 2018. Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia. Archives Animal Breeding 61: 481–89. DOI: https://doi.org/10.5194/aab-61-481-2018

Arango J G, Echeverri J Z and Lopez A H. 2014. Association of the bovine growth hormone gene with Holstein cattle reproductive parameters. Revista MVZ Cordoba 19(3): 4249–58. DOI: https://doi.org/10.21897/rmvz.87

Balogh O, Kovacs K, Kulcsar M, Gaspardy A, Febel H, Zsolnai A, Fesus C, Delavaud C, Chilliard Y, Gilbert R and Huszenicza G. 2009a. Interrelationship of growth hormone AluI polymorphism and hyperketonemia with plasma hormones and metabolites in the beginning of lactation in dairy cows. Livestock Science 123: 180–86. DOI: https://doi.org/10.1016/j.livsci.2008.11.002

Dahlin A, Khan U N, Zafar A H, Saleem M, Chaudhry M A and Philipsson J. 1998. Genetic and environmental causes of variation in milk production traits of Sahiwal cattle in Pakistan. Animal Science Journal 66: 307–18. DOI: https://doi.org/10.1017/S1357729800009437

Dhawan S, Yadav A S, Dhaka S S and Chakraborty D. 2015. Genetic studies on production and production efficiency traits in Sahiwal cattle. Indian Veterinary Journal 92(9): 35–38.

Dongre V B, Gandhi R S, Singh A, Sachdeva G K, Singh R K and Gupta A. 2013. Influence of non-genetic factors on fortnightly test day milk yields and first lactation 305-day milk yield in Sahiwal cattle. Indian Journal of Animal Research 47: 181–83. DOI: https://doi.org/10.5455/ijlr.20140423043653

Dybus A. 2002. Associations between Leu/Val polymorphism of growth hormone gene and milk production traits in Black and White cattle. Archiv fur Tierzucht 45: 421–28. DOI: https://doi.org/10.5194/aab-45-421-2002

El-Nahas A F, Basiony W M, El-Kassas S and Mahmoud S. 2018. Variation in the genetic effects of ABCG2, growth hormone and growth hormone receptor gene polymorphisms on milk production traits in Egyptian native, Holstein and Hybrid cattle populations. Pakistan Veterinary Journal 38(4): 371–76. DOI: https://doi.org/10.29261/pakvetj/2018.089

Falconer D S and Mackay T F C. 1996. Introduction to Quantitative Genetics. 4th ed. Addison Wesley Longman, Harlow.

Ferraz A L, Bortolossi J C, Curi R A, Ferro M I and Furlan L R. 2006. Identification and characterization of polymorph isms within the 5' flanking region, first exon and part of intron of bovine GH gene. Journal of Animal Breeding and Genetics 123: 208–12. DOI: https://doi.org/10.1111/j.1439-0388.2006.00574.x

Gorbani A, Vaez T, Bonyadi M and Amirinia C. 2009. A MspI PCR-RFLP within bovine growth hormone gene and its association with sperm quality traits in Iranian Holstein bulls. African Journal of Biotechnology 8 (19): 4811–16.

Harvey W R. 1990. Mixed Model Least squares and Maximum Likelihood computer Program (LSMLMW). PC-1 version.

Heidari M, Azari M A, Hasani S, Khanahmadi A and Zerehdaran S. 2012. Effect of polymorphic variants of GH, Pit-1, and - LG genes on milk production of Holstein cows. Russian Journal of Genetics 48: 417–21. DOI: https://doi.org/10.1134/S1022795412040060

Hoj S, Fredholm M, Larsen N J and Nielsen N H. 1993. Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle. Animal Genetics 24: 91–97. DOI: https://doi.org/10.1111/j.1365-2052.1993.tb00246.x

Kovacs K, Volgyi-Csik J, Zsolnai A, Gyorkos I and Fesus L. 2006. Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population. Archiv fur Tierzucht 49: 236–49. DOI: https://doi.org/10.5194/aab-49-236-2006

Larkin M A, Blackshields G, Brown N P, Chenna R, McGettigan P A, McWilliam H, Valentin F, Wallace I M, Wilm A, Lopez R, Thompson J D, Gibson T J and Higgins D G. 2007. Clustal W and Clustal X. Version 2.0. Bioinformatics 23: 2947–48. DOI: https://doi.org/10.1093/bioinformatics/btm404

Lee B K, Crooker B A, Hansen L B and Chester-Jones H. 1994. Polymorphism in the third intron of somatotropin (bST) gene and its association with selection for milk yield in Holstein cows. Journal of Animal Science 72: 316.

Lucy M C, Hauser S D, Eppard P J, Krivi G G, Clark J H, Bauman D E and Collier R J. 1993. Variants of somatotropin in cattle: gene frequencies in major dairy breeds and associated milk production. Domestic Animal Endocrinology 10: 325–33. DOI: https://doi.org/10.1016/0739-7240(93)90036-B

Manoj M, Gandhi R S, Raja T V, Singh A and Sachdeva G K. 2012. Effect of non-genetic factors on first lactation production and reproduction performance in Sahiwal cattle. Indian Journal of Dairy Science 65 (3): 264–65.

Mullen M, Berry D, Howard D, Diskin M, Lynch C, Berkowicz E, Magee D, MacHugh D and Waters S. 2010. Associations between novel single nucleotide polymorphisms in the Bos taurus growth hormone gene and performance traits in Holstein- Friesian dairy cattle. Journal of Dairy Science 93: 5959–69. DOI: https://doi.org/10.3168/jds.2010-3385

Ozdemir M, Kopuzlu S and Topal M. 2018. Relationships between milk protein polymorphisms and production traits in cattle: a systematic review and meta-analysis. Archives Animal Breeding 61: 197–206. DOI: https://doi.org/10.5194/aab-61-197-2018

Ozkan E. 2005. ‘An investigation on genetic structure of native and cultural cattle breeds in Turkey by microsatellite markers’. PhD Thesis, Trakya University, Tekirdag, Turkey.

Pandey M, Raja K N, Yousuf S and Gupta A K. 2019. Effect of non-genetic factors on First Lactation 305 days and Lifetime Milk Yield in Sahiwal cattle. Indian Journal of Dairy Science 72(1): 89–92. DOI: https://doi.org/10.33785/IJDS.2019.v72i01.011

Pawar R S, Tajane K R, Joshi C G, Rank D N and Bramkshtr B P. 2007. Growth hormone gene polymorphism and its association with lactation yield in dairy cattle. Indian Journal of Animal Science 77(9): 884–88.

Ramesha K P, Rao A, Basavaraju M, Geetha G R, Kataktalware M A and Jeyakumar S. 2015. Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo. South African Journal of Animal Science 45(5): 485–93. DOI: https://doi.org/10.4314/sajas.v45i5.5

Ratwan P, Chakravarty A K and Kumar M. 2019. Assessment of relation among production and reproduction traits in Sahiwal cattle at an organized herd of northern India. Biological Rhythm Research DOI: 10.1080/09291016.2019.1628391. DOI: https://doi.org/10.1080/09291016.2019.1628391

Sambrook J and Russell D. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

Singh J and Singh C V. 2016. Genetic and phenotypic parameters of first lactation and life time traits in Sahiwal cows. Journal of Veterinary Science and Technology, DOI:10.4172/2157- 7579.1000345. DOI: https://doi.org/10.4172/2157-7579.1000345

Sonmez C Z, Ozdemir M, Bayram B and Aksakal V. 2018. Relationships between GH/AluI polymorphism and some performance traits in Holstein. Turkish Journal of Agriculture - Food Science and Technology 6(5): 602–06. DOI: https://doi.org/10.24925/turjaf.v6i5.602-606.1838

Thidar M, Yoshida H, Ito H, He T, Inoue M and Kuwayama H. 2008. Combined administration of ghrelin and GHRH synergistically stimulates GH release in Holstein preweaning calves. Domestic Animal Endocrinology 34: 118–23. DOI: https://doi.org/10.1016/j.domaniend.2006.12.001

Vasconcellos L P M K, Tambasco D D, Pereira A P, Coutinho L L and Regitano L C A. 2003. Genetic characterization of Aberdeen Angus cattle using molecular markers. Genetics and Molecular Biology 26(2): 133–37. DOI: https://doi.org/10.1590/S1415-47572003000200005

Verma M K, Sachdeva G K, Yadav A K, Gautam S, Ali M M and Bindal S. 2016. Effect of genetic and nongenetic factors on milk yield and milk constituents on Sahiwal cattle. Indian Journal of Animal Research 50(5): 808–10. DOI: https://doi.org/10.18805/ijar.9297

Yao J, Aggrey S E, Zadworny D, Hayes J F and Kuhnlein U. 1996. Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphism (SSCP) analysis and their association with milk production traits in Holsteins. Genetics 144: 1809–16. DOI: https://doi.org/10.1093/genetics/144.4.1809

Zhang H M, Maddock K C, Brown D R, Denise S K and Ax R L. 1993. A novel of the bovine somatotropin gene detected by PCR-RFLP analysis. Animal Science 71: 2276. DOI: https://doi.org/10.2527/1993.7182276x

Zhou G L, Zhu Q, Jin H G, Liu C and Guo S. 2006. Genetic variation of growth hormone gene and its relationship with milk production traits in China Holstein cows. Asian- Australasian Journal of Animal Sciences 19: 315–18. DOI: https://doi.org/10.5713/ajas.2006.315

Zwierzchowski L, Oprzadek J, Dymnicki E and Dzierzbicki P. 2001. An association of growth hormone, kappa-casein, betalactoglobulin, leptin and pit-1 loci polymorphism with growth rate and carcass traits in beef cattle. Animal Science Papers and Reports 19: 65–77.