Supplementation of caffeine to extender improves post-thaw seminal attributes of Poitou donkey semen

SOURABH KANT; YASH PAL; R A LEGHA; S K RAVI; T R TALLURI; TARU SHARMA

doi:10.56093/ijans.v91i3.114141

Authors

SOURABH KANT ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
YASH PAL ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
R A LEGHA ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
S K RAVI ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
T R TALLURI ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India
TARU SHARMA ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v91i3.114141

Keywords:

Acrosome integrity, Caffeine, Cryopreservation, Jack sperm, Kinetic parameters

Abstract

The current study was conducted with an objective of elucidating the effect of addition of various levels of caffeine to jack semen on seminal attributes at pre- and post-thaw stage. Six ejaculates from each three adult jack stallions were collected via artificial vagina using estrus jenny as dummy. Caffeine was added to semen extender at 0.1, 0.5 and 2 mM at pre-freeze stage and the semen was cryopreserved according to standard protocol. Sperm motility, viability and other functional attributes were assessed at pre-freeze and post-thaw stages. The present study revealed that, adding caffeine to jack semen extender at 0.1 mM, 0.5 mM or 2 mM levels had positive impact on seminal parameters compared to the control semen. Sperm kinetics (VAP, VSL and STR) and motility increased in the caffeine treated groups in comparison to the control group. The caffeine at 2.0 mM concentration resulted in better post-thaw sperm motility, viability, and acrosome integrity as compared to 1 or 0.5 mM of caffeine and control. Caffeine supplementation also enhanced post-thaw sperm kinematic parameters, which implies its potential as an alternative antioxidant supplement. It was concluded that, 2 mM caffeine supplementation to donkey semen significantly improved post-thaw sperm motility and other functional attributes as compared to the control. However, the potential benefits of caffeine on the fertility rates needs to be investigated.

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