Supplementation of caffeine to extender improves post-thaw seminal attributes of Poitou donkey semen
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Keywords:
Acrosome integrity, Caffeine, Cryopreservation, Jack sperm, Kinetic parametersAbstract
The current study was conducted with an objective of elucidating the effect of addition of various levels of caffeine to jack semen on seminal attributes at pre- and post-thaw stage. Six ejaculates from each three adult jack stallions were collected via artificial vagina using estrus jenny as dummy. Caffeine was added to semen extender at 0.1, 0.5 and 2 mM at pre-freeze stage and the semen was cryopreserved according to standard protocol. Sperm motility, viability and other functional attributes were assessed at pre-freeze and post-thaw stages. The present study revealed that, adding caffeine to jack semen extender at 0.1 mM, 0.5 mM or 2 mM levels had positive impact on seminal parameters compared to the control semen. Sperm kinetics (VAP, VSL and STR) and motility increased in the caffeine treated groups in comparison to the control group. The caffeine at 2.0 mM concentration resulted in better post-thaw sperm motility, viability, and acrosome integrity as compared to 1 or 0.5 mM of caffeine and control. Caffeine supplementation also enhanced post-thaw sperm kinematic parameters, which implies its potential as an alternative antioxidant supplement. It was concluded that, 2 mM caffeine supplementation to donkey semen significantly improved post-thaw sperm motility and other functional attributes as compared to the control. However, the potential benefits of caffeine on the fertility rates needs to be investigated.Downloads
References
Aitken J and Fisher H. 1994. Reactive oxygen species generation and human spermatozoa – the balance of benefit and risk. Bioessays 16: 259–67. DOI: https://doi.org/10.1002/bies.950160409
Avanzi B R, Ramos R S, Araujo G H M, Fioratti E G, Trinca L A and Dell’Aqua Jr J A. 2015. Fixed-time insemination with frozen semen in mares: is it suitable for poorly fertile stallions? Theriogenology 83: 1389–93. DOI: https://doi.org/10.1016/j.theriogenology.2014.07.007
Barakat I A H, Danfour M A, Galewan F A M and Dkhil M A. 2015. Effect of various concentrations of caffeine, pentoxyfylline, and kallikrein on hyperactivation of frozen bovine semen. Biomedical Research International: 948575. DOI: https://doi.org/10.1155/2015/948575
Blottner S, Warnke C, Tuchscherer A, Heinen V and Torner H. 2001. Morphological and functional changes of stallion spermatozoa after cryopreservation during breeding and non- breeding season. Animal Reproduction Science 65: 75–88. DOI: https://doi.org/10.1016/S0378-4320(00)00214-1
Canisso I F, Carvalho G R, Morel M D, Ker P G, Rodrigues A L and Silva E C. 2011. Seminal parameters and field fertility of cryopreserved donkey jack semen after insemination of horse mares. Equine Veterinary Journal 43: 179e83. DOI: https://doi.org/10.1111/j.2042-3306.2010.00130.x
Carrel D T and Aston K I. 2013. Spermatogenesis: Methods and Protocols. New York, USA, Humana Press. DOI: https://doi.org/10.1007/978-1-62703-038-0
Colas C, Cebrian-Perez J A and Muino-Blanco T. 2010. Caffeine induces ram sperm hyperactivation independent of cAMP- dependent protein kinase. International Journal of Andrology 33: e187e97. DOI: https://doi.org/10.1111/j.1365-2605.2009.00991.x
Contri A, De Amicis I, Veronesi M C, Faustini M, Robbe D and Carluccio A. 2010. Efficiency of different extenders on cooled semen collected during long and short day length seasons in Martina Franca donkey. Animal Reproduction Science 120: 136–41. DOI: https://doi.org/10.1016/j.anireprosci.2010.02.018
El-Gaafary M N. 1994. Quality and fertility of cooled rabbit semen supplemented with cyclic-AMP stimulators. Animal Reproduction Science 34: 3070–313. DOI: https://doi.org/10.1016/0378-4320(94)90026-4
El-Shahat K H, Taysser M I, Badr M R and Zaki K A. Effect of heparin, caffeine and calcium ionophore A23187 on in vitro induction of the acrosome reaction of fresh ram spermatozoa. Asian Pacific Journal of Reproduction 5:148–55. DOI: https://doi.org/10.1016/j.apjr.2016.01.012
Karger S, Geiser B, Grau M, Heuwieser W and Arlt S P. 2017. Short communication: Progressive motility of frozen-thawed canine semen is highest five minutes after thawing. Reproduction Domestic Animals 52: 350–52. DOI: https://doi.org/10.1111/rda.12905
Kumar R, Ravi S K, Dholpuria S, Purohit G N, Pushp M K, Solanki S, Ganguly S and Pratap C. 2018. Study on fresh semen characteristics in donkeys. Journal of Entomology and Zoological Studies 61: 227e9.
Legha R A, Yash Pal, Ravi S K and Dedar R K. 2013. Physical and biochemical properties of Indian jack semen. Animal Science Reporter 7(4): 153–60.
Miró J, Vilés K, García W, Jordana J and Yeste M. 2013. Effect of donkey seminal plasma on sperm movement and sperm polymorphonuclear neutrophils attachment in vitro. Animal Reproduction Science 140: 164–72. DOI: https://doi.org/10.1016/j.anireprosci.2013.06.007
Mirshokraei P, Hassanpour H, Mehdizadeh A and Akhavan Taheri M. 2011. Pentoxifylline induces capacitation and acrosome reaction and improves quality of motility in canine ejaculated spermatozoa. Research in Veterinary Science 91: 281e4. DOI: https://doi.org/10.1016/j.rvsc.2011.01.002
Morris G J, Faszer K, Green J E, Draper D, Grout B W and Fonseca F. 2007. Rapidly cooled horse spermatozoa: loss of viability is due to osmotic imbalance during thawing, not intracellular ice formation. Theriogenology 68: 804–12. DOI: https://doi.org/10.1016/j.theriogenology.2007.06.009
Nabavi N, Todehdehghan F and Shiravi A. 2013. Effect of caffeine on motility and vitality of sperm and in vitro fertilization of outbreed mouse in T6 and M16 media. Iranian Journal of Reprodution and Medicine 11: 741–6.
Naz R K and Rajesh P B. 2004. Role of tyrosine phosphorylation in sperm capacitation/ acrosome reaction. Reproduction Biology Endocrinology 2: 75.
Ortiz I, Dorado J, Morrell J M, Crespo F, Gosálvez J and Gálvez M J. 2015. Effect of single-layer centrifugation or washing on frozen-thawed donkey semen quality: do they have the same effect regardless of the quality of the sample? Theriogenology 84: 294–300. DOI: https://doi.org/10.1016/j.theriogenology.2015.03.021
Prashant Kumar, Rabindra Kumar, Jitendra Singh Mehta, Ashok Kumar Chaudhary, Sanjay Kumar Ravi, Sharat Chandra Mehta, Mohd. Matin Ansari, Ram Avtar Legha, Bupendra Nath Tripathi and Thirumala Rao Talluri. 2019. Ameliorative effect of ascorbic acid and glutathione in combating the cryoinjuries during cryopreservation of exotic Jack semen. Journal of Equine Veterinary Science 81: 102796. DOI: https://doi.org/10.1016/j.jevs.2019.102796
Rota A, Magelli C, Panzani D and Camillo F. 2008. Effect of extender, centrifugation and removal of seminal plasma on cooled preserved Amiata donkey spermatozoa. Theriogenology 69: 176–85. DOI: https://doi.org/10.1016/j.theriogenology.2007.09.003
Rota A, Panzani D, Sabatini C and Camillo F. 2012. Donkey jack (Equus asinus) semen cryopreservation: studies of seminal parameters, post breeding inflammatory response, and fertility in donkey jennies. Theriogenology 78: 1846–54. DOI: https://doi.org/10.1016/j.theriogenology.2012.07.015
Shukla M K and Misra A K. 2014. Caffeine as a semen additive to improve Murrah buffalo (Bubalus bubalis) semen cryopreservation. Buffalo Bulletin 33: 32–36.
Soni Y, Talluri T R, Kumar A, Ravi S K, Mehta J S and Tripathi B N. 2019. Effects of different concentration and combinations of cryoprotectants on sperm quality, functional integrity in three Indian horse breeds. Cryobiology 86: 52e7. DOI: https://doi.org/10.1016/j.cryobiol.2018.12.005
Stephens T D, Brooks R M, Carrington J L, Cheng L, Carrington A C and Porr C A. 2013. Effects of pentoxifylline, caffeine, and taurine on post-thaw motility and longevity of equine frozen semen. Journal of Equine Veterinary Science 33: 615e21. DOI: https://doi.org/10.1016/j.jevs.2012.10.004
Tartaglione C M and Ritta M N. 2004. Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen. Theriogenology 62: 1245e52. DOI: https://doi.org/10.1016/j.theriogenology.2004.01.012
Watson P F. 2000. The causes of reduced fertility with cryopreservation semen. Animal Reproduction Science 60: 481–92. DOI: https://doi.org/10.1016/S0378-4320(00)00099-3
Yamaguchi S, Suzuki C, Noguchi M, Kasa S, Mori M, Isozaki Y, Ueda S, Funahashi H, Kikuchi K, Nagai T and Yoshioka K. 2013. Effects of caffeine on sperm characteristics after thawing and inflammatory response in the uterus after artificial insemination with frozen-thawed boar semen. Theriogenology 79: 87e93. DOI: https://doi.org/10.1016/j.theriogenology.2012.09.012
Zhang J, Guo H, Su J, Zhao L, Li Y, Sun W, Han H, Hu S, Zhao G, Li Y, Dai Y and Li X. 2014. A comparison of the effects of pentoxifylline on quality of fresh and frozen-thawed bull spermatozoa. Agriculture Biotechnology 3: 20–29.
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