Enrichment of motile spermatozoa from cattle semen samples by microfluidics method

Vinod Kumar Yata; Neeraj Yadav; Vibhav Katoch; Dharmendra Kumar Gangwar; Sudarshan Kumar; Tushar Kumar Mohanty; Bhanu Prakash; Ashok Kumar Mohanty

doi:10.56093/ijans.v92i6.114553

Authors

Vinod Kumar Yata Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal-132001, India
Neeraj Yadav Microfluidics Research Laboratory, Institute of Nano Science and Technology (INST), Phase 10, Sector 64, SAS Nagar-160062, India
Vibhav Katoch Microfluidics Research Laboratory, Institute of Nano Science and Technology (INST), Phase 10, Sector 64, SAS Nagar-160062, India
Dharmendra Kumar Gangwar Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal-132001, India
Sudarshan Kumar Animal Biotechnology Centre, National Dairy Research Institute (NDRI), Karnal-132001, India
Tushar Kumar Mohanty Artificial Breeding Research Centre, National Dairy Research Institute (NDRI), Karnal-132001, India
Bhanu Prakash Microfluidics Research Laboratory, Institute of Nano Science and Technology (INST), Phase 10, Sector 64, SAS Nagar-160062, India
Ashok Kumar Mohanty ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Mukteswar- 263138, District Nainital; State: Uttarakhand

https://doi.org/10.56093/ijans.v92i6.114553

Keywords:

Microfluidics, sperm separation, Motile sperm, CASA, livestock

Abstract

Motile sperm cell separation is important in sample preparation for both artificial insemination and cryopreservation of semen. A novel microfluidic device consisting of an inlet microchannel, a separating reservoir and two outlet microchannels was developed to enrich the motile sperm cells of cattle semen samples. Sperm separation was performed in this microfluidic device using a continuous flow process based on the swim up behaviour of motile cells. Separating reservoir allows the high motile sperm cells to swim up and pass through the top outlet of the reservoir. Low and non-motile sperm cells pass through the bottom outlet of the reservoir in the direction of fluid flow. The microfluidic device was fabricated using polydimethylsiloxane (PDMS) and semen samples were infused into the microfluidic device through a syringe pump. Sperm motility was analyzed by Computer-assisted sperm analysis (CASA). More than 80% enrichment of motile spermatozoa in the cattle semen samples was observed after their separation in the fabricated microfluidic device.

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Author Biography

Ashok Kumar Mohanty, ICAR-Indian Veterinary Research Institute, Mukteswar Campus, Mukteswar- 263138, District Nainital; State: Uttarakhand

Dr. Ashok Kumar Mohanty,Â Joint Director,ICAR-Indian Veterinary Research Institute,Â Mukteswar Campus, Mukteswar- 263138,District Nainital; State: Uttarakhand

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