Immunogenicity of chitosan coupled heat killed and fusion protein vaccine of Mycobacterium avium subsp. paratuberculosis

K VIJAYARANI; S SUDHA; B ABIRAMI; C V PRIYADHARSHINI; K KUMANAN

doi:10.56093/ijans.v91i6.115446

Authors

K VIJAYARANI Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
S SUDHA Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
B ABIRAMI Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
C V PRIYADHARSHINI Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
K KUMANAN Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

https://doi.org/10.56093/ijans.v91i6.115446

Keywords:

Heat killed vaccine, Johneâ€™s disease, Mycobacterium avium subsp. Paratuberculosis

Abstract

In search for better vaccine candidates for Johne's disease, chitosan nanoparticle coupled heat killed whole cell and fusion protein (Antigen 85A+85B) vaccines were developed using a local isolate of Mycobacterium avium subsp. paratuberculosis. Immunization studies in goats indicated that the heat killed whole cell vaccine and the fusion protein vaccine were found to be safe and resulted in Th1 and Th2 responses. Antibody response was significantly higher in the heat killed whole cell immunized animals than those received the fusion protein. Significant increase in heat killed whole cell MAP and fusion protein specific IFN-Î³ gene expression was detected in the immunized animals in contrast to the controls. The response was significantly higher with the heat killed whole cell MAP than the fusion protein. The adjuvant effect of chitosan nanoparticles was clearly evident in the second trial wherein the animals were observed for a longer period of 24 weeks. With increased IFN-Î³ and antibody levels, vaccinated animals responded well as compared to the control ones and the response was better in heat killed whole cell vaccine.

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References

Bastida F and Juste R A. 2011. Paratuberculosis control: A review with a focus on vaccination. Journal of Immune Based Therapies and Vaccines 9: 8. DOI: https://doi.org/10.1186/1476-8518-9-8

Gupta S, Singh S V, Singh M, Chaubey K K, Karthik K, Bhatia A K, Naveen Kumar and Dhama K. 2019. Vaccine approaches for the ‘therapeutic management’ of Mycobacterium avium subspecies paratuberculosis infection in domestic livestock. Veterinary Quarterly 39: 143–52. DOI: https://doi.org/10.1080/01652176.2019.1667042

Kumanan K, Park S U, McDonough S, Stehman S, Akey B, Huntley J, Wong S, Chang C F and Chang Y F. 2008. Vaccination with recombinant Mycobacterium avium subsp. paratuberculosis proteins induces differential immune responses and protects calves against infection by oral challenge. Vaccine 26: 1652–63. DOI: https://doi.org/10.1016/j.vaccine.2008.01.015

Shin S J, Chang C F, Chang C D, McDonough S P, Thompson B, Yoo H S and Chang Y F. 2005. In vitro cellular immune responses to recombinant antigens of Mycobacterium avium subsp. paratubeculosis. Infection and Immunity 73: 5074–85. DOI: https://doi.org/10.1128/IAI.73.8.5074-5085.2005

Singh S V, Singh A V, Singh P K, Gupta V K, Kumar S and Vohra J. 2007. Sero prevalence of paratuberculosis in young kids using ‘Bison type’ Mycobacterium avium subsp paratubeculosis antigen in plate ELISA. Small Ruminant Research 70: 89–92. DOI: https://doi.org/10.1016/j.smallrumres.2005.11.019

Singh S V, Sharma M C, Sohal J S, Singh A V and Singh P K. 2009. Johne’s disease status, diagnosis and control: Indian Scenario. Central Institute for Research on Goats, Makhdoom, India. pp 10–16.

Singh K, Chendel B S, Chauhan H C, Dadawala A, Singh S V and Singh P K. 2013. Efficacy of ‘indigenous vaccine’ using native ‘Indian bison type’ genotype of Mycobacterium avium subspecies paratuberculosis for the control of clinical Johne’s disease in an organized goat herd. Veterinary Research Communications 37: 109–14. DOI: https://doi.org/10.1007/s11259-013-9551-4

Uzonna J E, Chilton P, Whitlock R H, Habecker P L, Scott P and Sweeney R W. 2003. Efficacy of commercial and field strain Mycobacterium paratuberculosis vaccinations with recombinant IL-12 in a bovine experimental infection model. Vaccine 21: 3101–09. DOI: https://doi.org/10.1016/S0264-410X(03)00261-5

Vijayarani K, Shrine Nagalakshmi K, Balachandran C and Kumanan K. 2009. Simultaneous infection of Johne’s and tuberculosis in a cow. Indian Veterinary Journal 86: 960–61.

Zhu B D, Qie Y Q, Wang J L, Zhang Y, Wang Q Z, Xu Y and Wang H H. 2007. Chitosan microspheres enhance the immunogenicity of an ag85b-based fusion protein containing multiple t-cell epitopes of Mycobacterium tuberculosis. European Journal of Pharmaceutics Biopharmaceutics 66: 318–26. DOI: https://doi.org/10.1016/j.ejpb.2006.11.028