Expression of caspase-3, caspase-9, GDF-9 and IGF-1 genes in ovine oocytes cultured with optimum and elevated doses of Amphiregulin, Neuregulin-1 and Tumour necrosis factor-α in-vitro

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  • RAMESH HONDARAVALLI SOMASHETTY Karnataka Veterinary, Animal & Fisheries Sciences University, Bidar, Veterinary College, Hebbal, Bengaluru
  • NANDI SUMANTA NIANP-ICAR, Adugodi, Bengaluru
  • GIRISH KUMAR VENKATESH Karnataka Veterinary, Animal & Fisheries Sciences University, Bidar, Veterinary College, Hebbal, Bengaluru


Caspase-3, Caspase-9, GDF-9, IGF-1, Oocytes


The present study was conducted to evaluate the expression of caspase-3, caspase-9, growth differentiation factor-9 (GDF-9) and insulin growth factor-1 (IGF-1) genes in oocytes cultured in vitro with optimum and elevated doses of amphiregulin (50 ng and 150 ng), neuregulin-1 (25 ng and 150 ng) and tumor necrosis factor-α (25 ng and 150 ng) during in vitro maturation of oocytes based on the results of effects of AREG or NRG-1 or TNF-α concentration on maturation of oocytes which caused significant effect, were selected for gene expression studies along with the basal/control level. Total RNA was extracted from in vitro matured oocytes using Trizol method and Real-time reverse transcription polymerase chain reaction was used to evaluate the expression of genes. There was an upregulation of caspase-3 at 150 ng of TNF-α, caspase-9 at 50 ng of AREG, GDF-9 at 150 ng of AREG and IGF-1 at 150 ng of AREG and 25 ng of TNF-α. These results suggested that AREG at elevated dose and TNF-α at optimum dose enhanced the expression of GDF-9 and IGF-1, while the presence of elevated dose of TNF-α and optimum dose of AREG activated caspase-3 and caspase-9, respectively in oocytes cultured in vitro.


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Author Biographies

RAMESH HONDARAVALLI SOMASHETTY, Karnataka Veterinary, Animal & Fisheries Sciences University, Bidar, Veterinary College, Hebbal, Bengaluru

Department of Veterinary Biochemistry, Veterinary college, and  Assistant Professor.


NIANP-ICAR, Principal Scientist.

GIRISH KUMAR VENKATESH, Karnataka Veterinary, Animal & Fisheries Sciences University, Bidar, Veterinary College, Hebbal, Bengaluru

Department of Veterinary Biochemistry and Professor & Head


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How to Cite

SOMASHETTY, R. H., SUMANTA, N., & VENKATESH, G. K. (2022). Expression of caspase-3, caspase-9, GDF-9 and IGF-1 genes in ovine oocytes cultured with optimum and elevated doses of Amphiregulin, Neuregulin-1 and Tumour necrosis factor-α in-vitro. The Indian Journal of Animal Sciences, 92(9), 1051–1055.